Cargando…
Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean
INTRODUCTION: Soy isoflavone, a class of polyphenolic compounds exclusively occurred in legumes, is an important bioactive compound for both plants and human beings. The outline of isoflavones biosynthesis pathway has been drawn up basically in the previous research. However, research on the subject...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027745/ https://www.ncbi.nlm.nih.gov/pubmed/36959940 http://dx.doi.org/10.3389/fpls.2023.1014349 |
_version_ | 1784909775092318208 |
---|---|
author | Chen, Heng Liu, Changkai Li, Yansheng Wang, Xue Pan, Xiangwen Wang, Feifei Zhang, Qiuying |
author_facet | Chen, Heng Liu, Changkai Li, Yansheng Wang, Xue Pan, Xiangwen Wang, Feifei Zhang, Qiuying |
author_sort | Chen, Heng |
collection | PubMed |
description | INTRODUCTION: Soy isoflavone, a class of polyphenolic compounds exclusively occurred in legumes, is an important bioactive compound for both plants and human beings. The outline of isoflavones biosynthesis pathway has been drawn up basically in the previous research. However, research on the subject has been mostly restricted to investigate the static regulation of isoflavone content in soybean, rather than characterize its dynamic variation and modulation network in developing seeds. METHODS: In this study, by using eight recombinant inbred lines (RIL), the contents of six isoflavone components in the different stages of developing soybean seeds were determined to characterize the dynamic variation of isoflavones, and the isoflavones accumulation pattern at physiological level was investigated. Meanwhile, we integrated and analyzed the whole genome expression profile of four lines and 42 meta-transcriptome data, based on the multiple algorithms. RESULTS: This study: 1) obtained 4 molecular modules strongly correlated with isoflavone accumulation; 2) identified 28 novel major genes that could affect the accumulation of isoflavones in developing seeds free from the limitation of environments; 3) discussed the dynamic molecular patterns regulating isoflavones accumulation in developing seed; 4) expanded the isoflavone biosynthesis pathway. DISCUSSION: The results not only promote the understandings on the biosynthesis and regulation of isoflavones at physiological and molecular level, but also facilitate to breed elite soybean cultivars with high isoflavone contents. |
format | Online Article Text |
id | pubmed-10027745 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100277452023-03-22 Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean Chen, Heng Liu, Changkai Li, Yansheng Wang, Xue Pan, Xiangwen Wang, Feifei Zhang, Qiuying Front Plant Sci Plant Science INTRODUCTION: Soy isoflavone, a class of polyphenolic compounds exclusively occurred in legumes, is an important bioactive compound for both plants and human beings. The outline of isoflavones biosynthesis pathway has been drawn up basically in the previous research. However, research on the subject has been mostly restricted to investigate the static regulation of isoflavone content in soybean, rather than characterize its dynamic variation and modulation network in developing seeds. METHODS: In this study, by using eight recombinant inbred lines (RIL), the contents of six isoflavone components in the different stages of developing soybean seeds were determined to characterize the dynamic variation of isoflavones, and the isoflavones accumulation pattern at physiological level was investigated. Meanwhile, we integrated and analyzed the whole genome expression profile of four lines and 42 meta-transcriptome data, based on the multiple algorithms. RESULTS: This study: 1) obtained 4 molecular modules strongly correlated with isoflavone accumulation; 2) identified 28 novel major genes that could affect the accumulation of isoflavones in developing seeds free from the limitation of environments; 3) discussed the dynamic molecular patterns regulating isoflavones accumulation in developing seed; 4) expanded the isoflavone biosynthesis pathway. DISCUSSION: The results not only promote the understandings on the biosynthesis and regulation of isoflavones at physiological and molecular level, but also facilitate to breed elite soybean cultivars with high isoflavone contents. Frontiers Media S.A. 2023-03-07 /pmc/articles/PMC10027745/ /pubmed/36959940 http://dx.doi.org/10.3389/fpls.2023.1014349 Text en Copyright © 2023 Chen, Liu, Li, Wang, Pan, Wang and Zhang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Chen, Heng Liu, Changkai Li, Yansheng Wang, Xue Pan, Xiangwen Wang, Feifei Zhang, Qiuying Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean |
title | Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean |
title_full | Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean |
title_fullStr | Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean |
title_full_unstemmed | Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean |
title_short | Developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean |
title_sort | developmental dynamic transcriptome and systematic analysis reveal the major genes underlying isoflavone accumulation in soybean |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027745/ https://www.ncbi.nlm.nih.gov/pubmed/36959940 http://dx.doi.org/10.3389/fpls.2023.1014349 |
work_keys_str_mv | AT chenheng developmentaldynamictranscriptomeandsystematicanalysisrevealthemajorgenesunderlyingisoflavoneaccumulationinsoybean AT liuchangkai developmentaldynamictranscriptomeandsystematicanalysisrevealthemajorgenesunderlyingisoflavoneaccumulationinsoybean AT liyansheng developmentaldynamictranscriptomeandsystematicanalysisrevealthemajorgenesunderlyingisoflavoneaccumulationinsoybean AT wangxue developmentaldynamictranscriptomeandsystematicanalysisrevealthemajorgenesunderlyingisoflavoneaccumulationinsoybean AT panxiangwen developmentaldynamictranscriptomeandsystematicanalysisrevealthemajorgenesunderlyingisoflavoneaccumulationinsoybean AT wangfeifei developmentaldynamictranscriptomeandsystematicanalysisrevealthemajorgenesunderlyingisoflavoneaccumulationinsoybean AT zhangqiuying developmentaldynamictranscriptomeandsystematicanalysisrevealthemajorgenesunderlyingisoflavoneaccumulationinsoybean |