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Induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect

The utilization of antibiotic-loaded cement spacer in the induced membrane development has been a debate topic in human medicine. To the best of the author's knowledge, these combinations have not yet been evaluated in birds. Therefore, this study assessed induced membrane formation using radio...

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Autores principales: Campeiro Junior, Luiz D., Rahal, Sheila Canevese, Souza, Marcos A., Osowski, Alini, Silva Júnior, José I. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027929/
https://www.ncbi.nlm.nih.gov/pubmed/36960145
http://dx.doi.org/10.3389/fvets.2023.1027951
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author Campeiro Junior, Luiz D.
Rahal, Sheila Canevese
Souza, Marcos A.
Osowski, Alini
Silva Júnior, José I. S.
author_facet Campeiro Junior, Luiz D.
Rahal, Sheila Canevese
Souza, Marcos A.
Osowski, Alini
Silva Júnior, José I. S.
author_sort Campeiro Junior, Luiz D.
collection PubMed
description The utilization of antibiotic-loaded cement spacer in the induced membrane development has been a debate topic in human medicine. To the best of the author's knowledge, these combinations have not yet been evaluated in birds. Therefore, this study assessed induced membrane formation using radiography and histology, in a segmental defect of a chicken radius, with or without the addition of cefazolin. Thirty 18-month-old healthy chickens were divided into two equal groups: G1—bone defect filled with bone cement; G2—bone defect filled with cefazolin powder-loaded bone cement. Radiographic examinations of the left forearm were taken immediately after surgery and at 7, 15, and 21 postoperative days. For the collection of the induced membranes, five chickens in each group were euthanized at 7, 15, and 21 days after surgery. Radiographically, the bone cement was identified as a radiopaque structure occupying the bone defect in both groups. Mild new bone formation in at least one of the fractured extremities of the bone defect was seen only 21 days after surgery in most chickens. Histologically, there was no difference in the mean thickness of the induced membrane between groups at all time points. Multifocal multinucleated cells differed between groups at 7 (G1 > G2) and 21 (G2 > G1) days after surgery. Mononuclear inflammatory infiltrate differed between groups only on day 21 (G1 > G2). Fibrous tissue proliferation did not differ between groups at all evaluation times. Blood vessel density differed only at 21 days postoperatively (G2 < G1). Multifocal areas of cartilage differed between groups at all time points (G1 > G2). In conclusion, cefazolin mixed with bone cement did not affect thickness of the induced membrane, but did result in a negative effect on some histological aspects, such as fewer vessels, less multifocal areas of cartilage, and persistence of inflammation.
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spelling pubmed-100279292023-03-22 Induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect Campeiro Junior, Luiz D. Rahal, Sheila Canevese Souza, Marcos A. Osowski, Alini Silva Júnior, José I. S. Front Vet Sci Veterinary Science The utilization of antibiotic-loaded cement spacer in the induced membrane development has been a debate topic in human medicine. To the best of the author's knowledge, these combinations have not yet been evaluated in birds. Therefore, this study assessed induced membrane formation using radiography and histology, in a segmental defect of a chicken radius, with or without the addition of cefazolin. Thirty 18-month-old healthy chickens were divided into two equal groups: G1—bone defect filled with bone cement; G2—bone defect filled with cefazolin powder-loaded bone cement. Radiographic examinations of the left forearm were taken immediately after surgery and at 7, 15, and 21 postoperative days. For the collection of the induced membranes, five chickens in each group were euthanized at 7, 15, and 21 days after surgery. Radiographically, the bone cement was identified as a radiopaque structure occupying the bone defect in both groups. Mild new bone formation in at least one of the fractured extremities of the bone defect was seen only 21 days after surgery in most chickens. Histologically, there was no difference in the mean thickness of the induced membrane between groups at all time points. Multifocal multinucleated cells differed between groups at 7 (G1 > G2) and 21 (G2 > G1) days after surgery. Mononuclear inflammatory infiltrate differed between groups only on day 21 (G1 > G2). Fibrous tissue proliferation did not differ between groups at all evaluation times. Blood vessel density differed only at 21 days postoperatively (G2 < G1). Multifocal areas of cartilage differed between groups at all time points (G1 > G2). In conclusion, cefazolin mixed with bone cement did not affect thickness of the induced membrane, but did result in a negative effect on some histological aspects, such as fewer vessels, less multifocal areas of cartilage, and persistence of inflammation. Frontiers Media S.A. 2023-03-07 /pmc/articles/PMC10027929/ /pubmed/36960145 http://dx.doi.org/10.3389/fvets.2023.1027951 Text en Copyright © 2023 Campeiro Junior, Rahal, Souza, Osowski and Silva Júnior. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Campeiro Junior, Luiz D.
Rahal, Sheila Canevese
Souza, Marcos A.
Osowski, Alini
Silva Júnior, José I. S.
Induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect
title Induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect
title_full Induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect
title_fullStr Induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect
title_full_unstemmed Induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect
title_short Induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect
title_sort induced membrane technique using bone cement with or without cefazolin in chicken segmental radius defect
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10027929/
https://www.ncbi.nlm.nih.gov/pubmed/36960145
http://dx.doi.org/10.3389/fvets.2023.1027951
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