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Biogenic silver nanoparticles (AgNPs) from Tinosporacordifolia leaves: An effective antibiofilm agent against Staphylococcus aureus ATCC 23235

Medicinal plants are long known for their therapeutic applications. Tinospora cordifolia (commonly called gulancha or heart-leaved moonseed plant), a herbaceous creeper widely has been found to have antimicrobial, anti-inflammatory, anti-diabetic, and anti-cancer properties. However, there remains a...

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Autores principales: Ghosh, Sreejita, Mondol, Somdutta, Lahiri, Dibyajit, Nag, Moupriya, Sarkar, Tanmay, Pati, Siddhartha, Pandit, Soumya, Alarfaj, Abdullah A., Mohd Amin, Mohamad Faiz, Edinur, Hisham Atan, Ahmad Mohd Zain, Muhammad Rajaei, Ray, Rina Rani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028272/
https://www.ncbi.nlm.nih.gov/pubmed/36959877
http://dx.doi.org/10.3389/fchem.2023.1118454
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author Ghosh, Sreejita
Mondol, Somdutta
Lahiri, Dibyajit
Nag, Moupriya
Sarkar, Tanmay
Pati, Siddhartha
Pandit, Soumya
Alarfaj, Abdullah A.
Mohd Amin, Mohamad Faiz
Edinur, Hisham Atan
Ahmad Mohd Zain, Muhammad Rajaei
Ray, Rina Rani
author_facet Ghosh, Sreejita
Mondol, Somdutta
Lahiri, Dibyajit
Nag, Moupriya
Sarkar, Tanmay
Pati, Siddhartha
Pandit, Soumya
Alarfaj, Abdullah A.
Mohd Amin, Mohamad Faiz
Edinur, Hisham Atan
Ahmad Mohd Zain, Muhammad Rajaei
Ray, Rina Rani
author_sort Ghosh, Sreejita
collection PubMed
description Medicinal plants are long known for their therapeutic applications. Tinospora cordifolia (commonly called gulancha or heart-leaved moonseed plant), a herbaceous creeper widely has been found to have antimicrobial, anti-inflammatory, anti-diabetic, and anti-cancer properties. However, there remains a dearth of reports regarding its antibiofilm activities. In the present study, the anti-biofilm activities of phytoextractof T. cordifolia and the silver nanoparticles made from this phytoextract were tested against the biofilm of S.taphylococcus aureus, one of the major nosocomial infection-producing bacteria taking tetracycline antibiotic as control. Both phytoextract from the leaves of T. cordifolia, and the biogenic AgNPs from the leaf extract of T. cordifolia, were found successful in reducing the biofilm of Staphylococcus aureus. The biogenic AgNPs formed were characterized by UV- Vis spectroscopy, Field emission Scanning Electron Microscopy (FE- SEM), and Dynamic light scattering (DLS) technique. FE- SEM images showed that the AgNPs were of size ranging between 30 and 50 nm and were stable in nature, as depicted by the zeta potential analyzer. MIC values for phytoextract and AgNPs were found to be 180 mg/mL and 150 μg/mL against S. aureusrespectively. The antibiofilm properties of the AgNPs and phytoextract were analyzed using the CV assay and MTT assay for determining the reduction of biofilms. Reduction in viability count and revival of the S. aureus ATCC 23235 biofilm cells were analyzed followed by the enfeeblement of the EPS matrix to quantify the reduction in the contents of carbohydrates, proteins and eDNA. The SEM analyses clearly indicated that although the phytoextracts could destroy the biofilm network of S. aureuscells yet the biogenicallysynthesizedAgNPs were more effective in biofilm disruption. Fourier Transformed Infrared Radiations (FT- IR) analyses revealed that the AgNPs could bring about more exopolysaccharide (EPS) destruction in comparison to the phytoextract. The antibiofilm activities of AgNPs made from the phytoextract were found to be much more effective than the non-conjugated phytoextract, indicating the future prospect of using such particles for combatting biofilm-mediated infections caused by S aureus.
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spelling pubmed-100282722023-03-22 Biogenic silver nanoparticles (AgNPs) from Tinosporacordifolia leaves: An effective antibiofilm agent against Staphylococcus aureus ATCC 23235 Ghosh, Sreejita Mondol, Somdutta Lahiri, Dibyajit Nag, Moupriya Sarkar, Tanmay Pati, Siddhartha Pandit, Soumya Alarfaj, Abdullah A. Mohd Amin, Mohamad Faiz Edinur, Hisham Atan Ahmad Mohd Zain, Muhammad Rajaei Ray, Rina Rani Front Chem Chemistry Medicinal plants are long known for their therapeutic applications. Tinospora cordifolia (commonly called gulancha or heart-leaved moonseed plant), a herbaceous creeper widely has been found to have antimicrobial, anti-inflammatory, anti-diabetic, and anti-cancer properties. However, there remains a dearth of reports regarding its antibiofilm activities. In the present study, the anti-biofilm activities of phytoextractof T. cordifolia and the silver nanoparticles made from this phytoextract were tested against the biofilm of S.taphylococcus aureus, one of the major nosocomial infection-producing bacteria taking tetracycline antibiotic as control. Both phytoextract from the leaves of T. cordifolia, and the biogenic AgNPs from the leaf extract of T. cordifolia, were found successful in reducing the biofilm of Staphylococcus aureus. The biogenic AgNPs formed were characterized by UV- Vis spectroscopy, Field emission Scanning Electron Microscopy (FE- SEM), and Dynamic light scattering (DLS) technique. FE- SEM images showed that the AgNPs were of size ranging between 30 and 50 nm and were stable in nature, as depicted by the zeta potential analyzer. MIC values for phytoextract and AgNPs were found to be 180 mg/mL and 150 μg/mL against S. aureusrespectively. The antibiofilm properties of the AgNPs and phytoextract were analyzed using the CV assay and MTT assay for determining the reduction of biofilms. Reduction in viability count and revival of the S. aureus ATCC 23235 biofilm cells were analyzed followed by the enfeeblement of the EPS matrix to quantify the reduction in the contents of carbohydrates, proteins and eDNA. The SEM analyses clearly indicated that although the phytoextracts could destroy the biofilm network of S. aureuscells yet the biogenicallysynthesizedAgNPs were more effective in biofilm disruption. Fourier Transformed Infrared Radiations (FT- IR) analyses revealed that the AgNPs could bring about more exopolysaccharide (EPS) destruction in comparison to the phytoextract. The antibiofilm activities of AgNPs made from the phytoextract were found to be much more effective than the non-conjugated phytoextract, indicating the future prospect of using such particles for combatting biofilm-mediated infections caused by S aureus. Frontiers Media S.A. 2023-03-07 /pmc/articles/PMC10028272/ /pubmed/36959877 http://dx.doi.org/10.3389/fchem.2023.1118454 Text en Copyright © 2023 Ghosh, Mondol, Lahiri, Nag, Sarkar, Pati, Pandit, Alarfaj, Mohd Amin, Edinur, Ahmad Mohd Zain and Ray. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Ghosh, Sreejita
Mondol, Somdutta
Lahiri, Dibyajit
Nag, Moupriya
Sarkar, Tanmay
Pati, Siddhartha
Pandit, Soumya
Alarfaj, Abdullah A.
Mohd Amin, Mohamad Faiz
Edinur, Hisham Atan
Ahmad Mohd Zain, Muhammad Rajaei
Ray, Rina Rani
Biogenic silver nanoparticles (AgNPs) from Tinosporacordifolia leaves: An effective antibiofilm agent against Staphylococcus aureus ATCC 23235
title Biogenic silver nanoparticles (AgNPs) from Tinosporacordifolia leaves: An effective antibiofilm agent against Staphylococcus aureus ATCC 23235
title_full Biogenic silver nanoparticles (AgNPs) from Tinosporacordifolia leaves: An effective antibiofilm agent against Staphylococcus aureus ATCC 23235
title_fullStr Biogenic silver nanoparticles (AgNPs) from Tinosporacordifolia leaves: An effective antibiofilm agent against Staphylococcus aureus ATCC 23235
title_full_unstemmed Biogenic silver nanoparticles (AgNPs) from Tinosporacordifolia leaves: An effective antibiofilm agent against Staphylococcus aureus ATCC 23235
title_short Biogenic silver nanoparticles (AgNPs) from Tinosporacordifolia leaves: An effective antibiofilm agent against Staphylococcus aureus ATCC 23235
title_sort biogenic silver nanoparticles (agnps) from tinosporacordifolia leaves: an effective antibiofilm agent against staphylococcus aureus atcc 23235
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028272/
https://www.ncbi.nlm.nih.gov/pubmed/36959877
http://dx.doi.org/10.3389/fchem.2023.1118454
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