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Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics
INTRODUCTION: Innate lymphoid cells (ILCs) are key components of the immune system, yet the similarity and distinction of the properties across tissues under homeostasis, inflammation and tumor process remain elusive. METHODS: Here we performed integrative inference of ILCs to reveal their transcrip...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028295/ https://www.ncbi.nlm.nih.gov/pubmed/36960063 http://dx.doi.org/10.3389/fimmu.2023.1127413 |
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author | Song, Peng Cao, Ke Mao, Yonghuan Ai, Shichao Sun, Feng Hu, Qiongyuan Liu, Song Wang, Meng Lu, Xiaofeng Guan, Wenxian Shen, Xiaofei |
author_facet | Song, Peng Cao, Ke Mao, Yonghuan Ai, Shichao Sun, Feng Hu, Qiongyuan Liu, Song Wang, Meng Lu, Xiaofeng Guan, Wenxian Shen, Xiaofei |
author_sort | Song, Peng |
collection | PubMed |
description | INTRODUCTION: Innate lymphoid cells (ILCs) are key components of the immune system, yet the similarity and distinction of the properties across tissues under homeostasis, inflammation and tumor process remain elusive. METHODS: Here we performed integrative inference of ILCs to reveal their transcriptional profiles and heterogeneity from single-cell genomics. We collected a large number of ILCs from human six different tissues which can represent unique immune niches (circulation, lymphoid tissue, normal and inflamed mucosa, tumor microenvironment), to systematically address the transcriptional imprinting. RESULTS: ILCs are profoundly imprinted by their organ of residence, and tissue-specific distinctions are apparent under pathological conditions. In the hepatocellular carcinoma microenvironment, we identified intermediate c-kit(+) ILC2 population, and lin(-)CD127(-) NK-like cells that expressed markers of cytotoxicity including CCL5 and IFNG. Additionally, CD127(+)CD94(+) ILC1s were preferentially enriched in inflamed ileum from patients with Crohn’s disease. DISCUSSION: These analyses depicted a comprehensive characterization of ILC anatomical distribution and subset heterogeneity, and provided a base line for future temporal or spatial studies focused on tissue-specific ILC-mediated immunity. |
format | Online Article Text |
id | pubmed-10028295 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100282952023-03-22 Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics Song, Peng Cao, Ke Mao, Yonghuan Ai, Shichao Sun, Feng Hu, Qiongyuan Liu, Song Wang, Meng Lu, Xiaofeng Guan, Wenxian Shen, Xiaofei Front Immunol Immunology INTRODUCTION: Innate lymphoid cells (ILCs) are key components of the immune system, yet the similarity and distinction of the properties across tissues under homeostasis, inflammation and tumor process remain elusive. METHODS: Here we performed integrative inference of ILCs to reveal their transcriptional profiles and heterogeneity from single-cell genomics. We collected a large number of ILCs from human six different tissues which can represent unique immune niches (circulation, lymphoid tissue, normal and inflamed mucosa, tumor microenvironment), to systematically address the transcriptional imprinting. RESULTS: ILCs are profoundly imprinted by their organ of residence, and tissue-specific distinctions are apparent under pathological conditions. In the hepatocellular carcinoma microenvironment, we identified intermediate c-kit(+) ILC2 population, and lin(-)CD127(-) NK-like cells that expressed markers of cytotoxicity including CCL5 and IFNG. Additionally, CD127(+)CD94(+) ILC1s were preferentially enriched in inflamed ileum from patients with Crohn’s disease. DISCUSSION: These analyses depicted a comprehensive characterization of ILC anatomical distribution and subset heterogeneity, and provided a base line for future temporal or spatial studies focused on tissue-specific ILC-mediated immunity. Frontiers Media S.A. 2023-03-07 /pmc/articles/PMC10028295/ /pubmed/36960063 http://dx.doi.org/10.3389/fimmu.2023.1127413 Text en Copyright © 2023 Song, Cao, Mao, Ai, Sun, Hu, Liu, Wang, Lu, Guan and Shen https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Song, Peng Cao, Ke Mao, Yonghuan Ai, Shichao Sun, Feng Hu, Qiongyuan Liu, Song Wang, Meng Lu, Xiaofeng Guan, Wenxian Shen, Xiaofei Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics |
title | Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics |
title_full | Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics |
title_fullStr | Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics |
title_full_unstemmed | Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics |
title_short | Tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics |
title_sort | tissue specific imprinting on innate lymphoid cells during homeostasis and disease process revealed by integrative inference of single-cell transcriptomics |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028295/ https://www.ncbi.nlm.nih.gov/pubmed/36960063 http://dx.doi.org/10.3389/fimmu.2023.1127413 |
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