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Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate

OBJECTIVE: To explore a morphometric grading system for blastocysts that is associated with ongoing pregnancy. DESIGN: Cross-sectional study. SETTING: None. PATIENTS(S): All consecutive vitrified blastocysts at our center from July 2018 to November 2021 that were transferred in single blastocyst tra...

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Autores principales: Utsuno, Hiroki, Ishimaru, Tomoko, Matsumoto, Miho, Sasamori, Chiharu, Takahashi, Hikaru, Kimura, Hiroko, Kamijo, Shintaro, Yamada, Mitsutoshi, Tanaka, Mamoru, Hamatani, Toshio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028418/
https://www.ncbi.nlm.nih.gov/pubmed/36959960
http://dx.doi.org/10.1016/j.xfre.2022.11.001
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author Utsuno, Hiroki
Ishimaru, Tomoko
Matsumoto, Miho
Sasamori, Chiharu
Takahashi, Hikaru
Kimura, Hiroko
Kamijo, Shintaro
Yamada, Mitsutoshi
Tanaka, Mamoru
Hamatani, Toshio
author_facet Utsuno, Hiroki
Ishimaru, Tomoko
Matsumoto, Miho
Sasamori, Chiharu
Takahashi, Hikaru
Kimura, Hiroko
Kamijo, Shintaro
Yamada, Mitsutoshi
Tanaka, Mamoru
Hamatani, Toshio
author_sort Utsuno, Hiroki
collection PubMed
description OBJECTIVE: To explore a morphometric grading system for blastocysts that is associated with ongoing pregnancy. DESIGN: Cross-sectional study. SETTING: None. PATIENTS(S): All consecutive vitrified blastocysts at our center from July 2018 to November 2021 that were transferred in single blastocyst transfer cycles until January 2022. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The ongoing pregnancy rate after a single vitrified-warmed blastocyst transfer. Interobserver agreement on morphometric values among embryologists. RESULT(S): Three morphometric variables (blastocyst diameter, area of inner cell mass [ICM], and the estimated trophectoderm cell count) were used to evaluate the expansion, ICM, and trophectoderm morphology. During the study period, 585 blastocysts were involved in this study. Of the 3 morphometric variables, ICM area (per 500 μm(2), adjusted odds ratio, 1.19; 95% confidence interval, 1.09−1.30) and estimated trophectoderm cell count (per 10 cells, adjusted odds ratio, 1.25; 95% confidence interval, 1.12−1.39) were significantly associated with the ongoing pregnancy rate after adjustment for confounding factors. The ongoing pregnancy rate was 2.0% (1/49) with an ICM area of <2,500 μm(2) and the estimated trophectoderm cell count <70. The ongoing pregnancy rate reached 47.8% (22/46) when the ICM area and the estimated trophectoderm cell count were >3,500 μm(2) and >110, respectively. Interobserver agreement on the blastocyst diameter, ICM area, and the estimated trophectoderm cell count was excellent-to-good among 5 embryologists (intraclass correlation coefficients: 0.99, 0.87, and 0.91, respectively). CONCLUSION(S): Morphometric values of ICM and trophectoderm are promising predictors of pregnancy success. The high reproducibility suggests that the morphometric variables will contribute to identifying blastocysts with the highest developmental potential as well as those that will not result in a successful pregnancy.
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spelling pubmed-100284182023-03-22 Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate Utsuno, Hiroki Ishimaru, Tomoko Matsumoto, Miho Sasamori, Chiharu Takahashi, Hikaru Kimura, Hiroko Kamijo, Shintaro Yamada, Mitsutoshi Tanaka, Mamoru Hamatani, Toshio F S Rep Original Article OBJECTIVE: To explore a morphometric grading system for blastocysts that is associated with ongoing pregnancy. DESIGN: Cross-sectional study. SETTING: None. PATIENTS(S): All consecutive vitrified blastocysts at our center from July 2018 to November 2021 that were transferred in single blastocyst transfer cycles until January 2022. INTERVENTION(S): None. MAIN OUTCOME MEASURE(S): The ongoing pregnancy rate after a single vitrified-warmed blastocyst transfer. Interobserver agreement on morphometric values among embryologists. RESULT(S): Three morphometric variables (blastocyst diameter, area of inner cell mass [ICM], and the estimated trophectoderm cell count) were used to evaluate the expansion, ICM, and trophectoderm morphology. During the study period, 585 blastocysts were involved in this study. Of the 3 morphometric variables, ICM area (per 500 μm(2), adjusted odds ratio, 1.19; 95% confidence interval, 1.09−1.30) and estimated trophectoderm cell count (per 10 cells, adjusted odds ratio, 1.25; 95% confidence interval, 1.12−1.39) were significantly associated with the ongoing pregnancy rate after adjustment for confounding factors. The ongoing pregnancy rate was 2.0% (1/49) with an ICM area of <2,500 μm(2) and the estimated trophectoderm cell count <70. The ongoing pregnancy rate reached 47.8% (22/46) when the ICM area and the estimated trophectoderm cell count were >3,500 μm(2) and >110, respectively. Interobserver agreement on the blastocyst diameter, ICM area, and the estimated trophectoderm cell count was excellent-to-good among 5 embryologists (intraclass correlation coefficients: 0.99, 0.87, and 0.91, respectively). CONCLUSION(S): Morphometric values of ICM and trophectoderm are promising predictors of pregnancy success. The high reproducibility suggests that the morphometric variables will contribute to identifying blastocysts with the highest developmental potential as well as those that will not result in a successful pregnancy. Elsevier 2022-11-11 /pmc/articles/PMC10028418/ /pubmed/36959960 http://dx.doi.org/10.1016/j.xfre.2022.11.001 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Article
Utsuno, Hiroki
Ishimaru, Tomoko
Matsumoto, Miho
Sasamori, Chiharu
Takahashi, Hikaru
Kimura, Hiroko
Kamijo, Shintaro
Yamada, Mitsutoshi
Tanaka, Mamoru
Hamatani, Toshio
Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate
title Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate
title_full Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate
title_fullStr Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate
title_full_unstemmed Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate
title_short Morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate
title_sort morphometric assessment of blastocysts: relationship with the ongoing pregnancy rate
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028418/
https://www.ncbi.nlm.nih.gov/pubmed/36959960
http://dx.doi.org/10.1016/j.xfre.2022.11.001
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