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Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study

AIM: To assess the association of the expression of apolipoprotein B (apoB) and 4-hydroxynonenal (4HNE) with the clinicopathological data of patients with colorectal cancer (CRC). METHODS: We obtained 80 CRC histopathological specimens sent to the Pathology Laboratory of Hospital Universiti Sains Ma...

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Autores principales: Ng, Phei Ying, Nafi, Siti Norasikin Mohd, Jalil, Nur Asyilla Che, Kueh, Yee Cheng, Lee, Yeong Yeh, Zin, Anani Aila Mat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Croatian Medical Schools 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028567/
https://www.ncbi.nlm.nih.gov/pubmed/36864816
http://dx.doi.org/10.3325/cmj.2023.64.29
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author Ng, Phei Ying
Nafi, Siti Norasikin Mohd
Jalil, Nur Asyilla Che
Kueh, Yee Cheng
Lee, Yeong Yeh
Zin, Anani Aila Mat
author_facet Ng, Phei Ying
Nafi, Siti Norasikin Mohd
Jalil, Nur Asyilla Che
Kueh, Yee Cheng
Lee, Yeong Yeh
Zin, Anani Aila Mat
author_sort Ng, Phei Ying
collection PubMed
description AIM: To assess the association of the expression of apolipoprotein B (apoB) and 4-hydroxynonenal (4HNE) with the clinicopathological data of patients with colorectal cancer (CRC). METHODS: We obtained 80 CRC histopathological specimens sent to the Pathology Laboratory of Hospital Universiti Sains Malaysia from 2015 to 2019. Data on demographic factors, body mass index (BMI), and clinicopathological characteristics were also collected. Formalin-fixed paraffin-embedded tissues were stained by using an optimized immunohistochemical protocol. RESULTS: Patients were mostly older than 50 years, male, Malay, and overweight or obese. A high apoB expression was observed in 87.5% CRC samples (70/80), while a high 4HNE expression was observed in only 17.5% (14/80) of CRCs. The expression of apoB was significantly associated with the sigmoid and rectosigmoid tumor sites (p =0.001) and tumor size 3-5 cm (p =0.005). 4HNE expression was significantly associated with tumor size 3-5 cm (p =0.045). Other variables were not significantly associated with the expression of either marker. CONCLUSION: ApoB and 4HNE proteins may play a role in promoting CRC carcinogenesis.
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spelling pubmed-100285672023-03-22 Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study Ng, Phei Ying Nafi, Siti Norasikin Mohd Jalil, Nur Asyilla Che Kueh, Yee Cheng Lee, Yeong Yeh Zin, Anani Aila Mat Croat Med J Research Article AIM: To assess the association of the expression of apolipoprotein B (apoB) and 4-hydroxynonenal (4HNE) with the clinicopathological data of patients with colorectal cancer (CRC). METHODS: We obtained 80 CRC histopathological specimens sent to the Pathology Laboratory of Hospital Universiti Sains Malaysia from 2015 to 2019. Data on demographic factors, body mass index (BMI), and clinicopathological characteristics were also collected. Formalin-fixed paraffin-embedded tissues were stained by using an optimized immunohistochemical protocol. RESULTS: Patients were mostly older than 50 years, male, Malay, and overweight or obese. A high apoB expression was observed in 87.5% CRC samples (70/80), while a high 4HNE expression was observed in only 17.5% (14/80) of CRCs. The expression of apoB was significantly associated with the sigmoid and rectosigmoid tumor sites (p =0.001) and tumor size 3-5 cm (p =0.005). 4HNE expression was significantly associated with tumor size 3-5 cm (p =0.045). Other variables were not significantly associated with the expression of either marker. CONCLUSION: ApoB and 4HNE proteins may play a role in promoting CRC carcinogenesis. Croatian Medical Schools 2023-02 /pmc/articles/PMC10028567/ /pubmed/36864816 http://dx.doi.org/10.3325/cmj.2023.64.29 Text en Copyright © 2023 by the Croatian Medical Journal. All rights reserved. https://creativecommons.org/licenses/by/2.5/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Ng, Phei Ying
Nafi, Siti Norasikin Mohd
Jalil, Nur Asyilla Che
Kueh, Yee Cheng
Lee, Yeong Yeh
Zin, Anani Aila Mat
Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study
title Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study
title_full Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study
title_fullStr Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study
title_full_unstemmed Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study
title_short Immunohistochemical expression of apolipoprotein B and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study
title_sort immunohistochemical expression of apolipoprotein b and 4-hydroxynonenal proteins in colorectal carcinoma patients: a retrospective study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10028567/
https://www.ncbi.nlm.nih.gov/pubmed/36864816
http://dx.doi.org/10.3325/cmj.2023.64.29
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