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Characterization of a novel papillomavirus identified from a whale (Delphinapterus leucas) pharyngeal metagenomic library

Here, using viral metagenomic method, a novel whale papillomavirus (temporarily named wPV, GenBank accession number OP856597) was discovered in a whale (Delphinapterus leucas) pharyngeal metagenomic library. The complete genome size of wPV is 7179 bp, with GC content of 54.4% and a nucleotide compos...

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Autores principales: Lu, Xiang, Zhu, Rong, Dai, Ziyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10029273/
https://www.ncbi.nlm.nih.gov/pubmed/36941650
http://dx.doi.org/10.1186/s12985-023-02009-y
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author Lu, Xiang
Zhu, Rong
Dai, Ziyuan
author_facet Lu, Xiang
Zhu, Rong
Dai, Ziyuan
author_sort Lu, Xiang
collection PubMed
description Here, using viral metagenomic method, a novel whale papillomavirus (temporarily named wPV, GenBank accession number OP856597) was discovered in a whale (Delphinapterus leucas) pharyngeal metagenomic library. The complete genome size of wPV is 7179 bp, with GC content of 54.4% and a nucleotide composition of 23.4% A, 22.3% T, 28.4% G, and 25.9% C. The viral genome has a typical papillomavirus organization pattern, and five ORFs were predicted, including two late genes encoding L1 and L2, and three early genes encoding E1, E2, and E6. Pairwise sequence comparison and phylogenetic analysis based on the L1 gene sequence indicated that wPV may be a novel species within genus Dyodeltapapillomavirus. In addition, the E2 region of wPV was predicted to have a potential recombination event. The discovery of this novel papillomavirus increases our understanding of the viral ecology of marine mammals, providing insights into possible future infectious diseases. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-023-02009-y.
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spelling pubmed-100292732023-03-22 Characterization of a novel papillomavirus identified from a whale (Delphinapterus leucas) pharyngeal metagenomic library Lu, Xiang Zhu, Rong Dai, Ziyuan Virol J Research Here, using viral metagenomic method, a novel whale papillomavirus (temporarily named wPV, GenBank accession number OP856597) was discovered in a whale (Delphinapterus leucas) pharyngeal metagenomic library. The complete genome size of wPV is 7179 bp, with GC content of 54.4% and a nucleotide composition of 23.4% A, 22.3% T, 28.4% G, and 25.9% C. The viral genome has a typical papillomavirus organization pattern, and five ORFs were predicted, including two late genes encoding L1 and L2, and three early genes encoding E1, E2, and E6. Pairwise sequence comparison and phylogenetic analysis based on the L1 gene sequence indicated that wPV may be a novel species within genus Dyodeltapapillomavirus. In addition, the E2 region of wPV was predicted to have a potential recombination event. The discovery of this novel papillomavirus increases our understanding of the viral ecology of marine mammals, providing insights into possible future infectious diseases. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12985-023-02009-y. BioMed Central 2023-03-20 /pmc/articles/PMC10029273/ /pubmed/36941650 http://dx.doi.org/10.1186/s12985-023-02009-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Lu, Xiang
Zhu, Rong
Dai, Ziyuan
Characterization of a novel papillomavirus identified from a whale (Delphinapterus leucas) pharyngeal metagenomic library
title Characterization of a novel papillomavirus identified from a whale (Delphinapterus leucas) pharyngeal metagenomic library
title_full Characterization of a novel papillomavirus identified from a whale (Delphinapterus leucas) pharyngeal metagenomic library
title_fullStr Characterization of a novel papillomavirus identified from a whale (Delphinapterus leucas) pharyngeal metagenomic library
title_full_unstemmed Characterization of a novel papillomavirus identified from a whale (Delphinapterus leucas) pharyngeal metagenomic library
title_short Characterization of a novel papillomavirus identified from a whale (Delphinapterus leucas) pharyngeal metagenomic library
title_sort characterization of a novel papillomavirus identified from a whale (delphinapterus leucas) pharyngeal metagenomic library
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10029273/
https://www.ncbi.nlm.nih.gov/pubmed/36941650
http://dx.doi.org/10.1186/s12985-023-02009-y
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