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Isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells

Excessive reactive oxygen species (ROS) in nucleus pulposus cells (NPCs) promote extracellular matrix (ECM) degradation and cellular inflammatory responses by activating a variety of cellular pathways, ultimately inducing cell apoptosis and leading to the development of low back pain. Here, we desig...

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Autores principales: Yu, Hao, Teng, Yun, Ge, Jun, Yang, Ming, Xie, Haifeng, Wu, Tianyi, Yan, Qi, Jia, Mengting, Zhu, Qing, Shen, Yanping, Zhang, Lianxue, Zou, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10029295/
https://www.ncbi.nlm.nih.gov/pubmed/36941611
http://dx.doi.org/10.1186/s12951-023-01856-9
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author Yu, Hao
Teng, Yun
Ge, Jun
Yang, Ming
Xie, Haifeng
Wu, Tianyi
Yan, Qi
Jia, Mengting
Zhu, Qing
Shen, Yanping
Zhang, Lianxue
Zou, Jun
author_facet Yu, Hao
Teng, Yun
Ge, Jun
Yang, Ming
Xie, Haifeng
Wu, Tianyi
Yan, Qi
Jia, Mengting
Zhu, Qing
Shen, Yanping
Zhang, Lianxue
Zou, Jun
author_sort Yu, Hao
collection PubMed
description Excessive reactive oxygen species (ROS) in nucleus pulposus cells (NPCs) promote extracellular matrix (ECM) degradation and cellular inflammatory responses by activating a variety of cellular pathways, ultimately inducing cell apoptosis and leading to the development of low back pain. Here, we designed and fabricated an isoginkgetin-loaded ROS-responsive delivery system (IGK@SeNP) based on diselenide block copolymers. Successfully encapsulated IGK was released intelligently and rapidly in a microenvironment with high ROS levels in degenerative disc. Controlled-release IGK not only efficiently scavenged ROS from the intervertebral disc together with diselenide block copolymers but also effectively enhanced autophagy in NPCs to inhibit ECM degradation and cell apoptosis, and showed significant therapeutic effects in the rat intervertebral disc degeneration (IDD) model. Overall, the synergistic effects of IGK@SeNP in ROS scavenging and autophagy enhancement endowed it with an attractive therapeutic strategy for IDD treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-023-01856-9.
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spelling pubmed-100292952023-03-22 Isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells Yu, Hao Teng, Yun Ge, Jun Yang, Ming Xie, Haifeng Wu, Tianyi Yan, Qi Jia, Mengting Zhu, Qing Shen, Yanping Zhang, Lianxue Zou, Jun J Nanobiotechnology Research Excessive reactive oxygen species (ROS) in nucleus pulposus cells (NPCs) promote extracellular matrix (ECM) degradation and cellular inflammatory responses by activating a variety of cellular pathways, ultimately inducing cell apoptosis and leading to the development of low back pain. Here, we designed and fabricated an isoginkgetin-loaded ROS-responsive delivery system (IGK@SeNP) based on diselenide block copolymers. Successfully encapsulated IGK was released intelligently and rapidly in a microenvironment with high ROS levels in degenerative disc. Controlled-release IGK not only efficiently scavenged ROS from the intervertebral disc together with diselenide block copolymers but also effectively enhanced autophagy in NPCs to inhibit ECM degradation and cell apoptosis, and showed significant therapeutic effects in the rat intervertebral disc degeneration (IDD) model. Overall, the synergistic effects of IGK@SeNP in ROS scavenging and autophagy enhancement endowed it with an attractive therapeutic strategy for IDD treatment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12951-023-01856-9. BioMed Central 2023-03-21 /pmc/articles/PMC10029295/ /pubmed/36941611 http://dx.doi.org/10.1186/s12951-023-01856-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Yu, Hao
Teng, Yun
Ge, Jun
Yang, Ming
Xie, Haifeng
Wu, Tianyi
Yan, Qi
Jia, Mengting
Zhu, Qing
Shen, Yanping
Zhang, Lianxue
Zou, Jun
Isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells
title Isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells
title_full Isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells
title_fullStr Isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells
title_full_unstemmed Isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells
title_short Isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells
title_sort isoginkgetin-loaded reactive oxygen species scavenging nanoparticles ameliorate intervertebral disc degeneration via enhancing autophagy in nucleus pulposus cells
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10029295/
https://www.ncbi.nlm.nih.gov/pubmed/36941611
http://dx.doi.org/10.1186/s12951-023-01856-9
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