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A Novel Strategy for Liposomal Drug Separation in Plasma by TiO(2) Microspheres and Application in Pharmacokinetics

PURPOSE: Liposomes are nano-scale materials with a biofilm-like structure. They have excellent biocompatibility and are increasingly useful in drug delivery systems. However, the in vivo fate of liposomal drugs is still unclear because existing bioanalytical methods for quantitation of total and lip...

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Autores principales: Yu, Yue-yang, Yuan, Mei, Qin, Wei-jie, Bai, Hai-hong, Liu, Hong-zhuo, Che, Jin-jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10029972/
https://www.ncbi.nlm.nih.gov/pubmed/36960125
http://dx.doi.org/10.2147/IJN.S396746
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author Yu, Yue-yang
Yuan, Mei
Qin, Wei-jie
Bai, Hai-hong
Liu, Hong-zhuo
Che, Jin-jing
author_facet Yu, Yue-yang
Yuan, Mei
Qin, Wei-jie
Bai, Hai-hong
Liu, Hong-zhuo
Che, Jin-jing
author_sort Yu, Yue-yang
collection PubMed
description PURPOSE: Liposomes are nano-scale materials with a biofilm-like structure. They have excellent biocompatibility and are increasingly useful in drug delivery systems. However, the in vivo fate of liposomal drugs is still unclear because existing bioanalytical methods for quantitation of total and liposomal-encapsulated drugs have limits. A novel strategy for liposomal-encapsulated drug separation from plasma was developed via the specific coordinate binding interaction of TiO(2) microspheres with the phosphate groups of liposomes. METHODS: Liposomal-encapsulated docetaxel was separated from plasma by TiO(2) microspheres and analyzed by the UPLC-MS/MS method. The amount of TiO(2), pH of the dilutions, plasma dilution factors and incubation time were optimized to improve extraction recovery. The characterization of the adsorption of liposome-encapsulated drugs by TiO(2) microspheres was observed by electron microscopy. For understanding the mechanism, pseudo-first and the pseudo-second order equations were proposed for the adsorption process. The study fully validated the method for quantitation of liposomal-encapsulated in plasma and the method was applied to the pharmacokinetic study of docetaxel liposomes. RESULTS: The encapsulated docetaxel had a concentration range of 15–4000 ng/mL from the plasma sample using a TiO(2) extraction method. Successful method validation proved the method was sensitive, selective and stable, and was suitable for quantitation of docetaxel liposomes in plasma samples. Extraction recovery of this method was higher than that of SPE method. As shown in electron microscopy, the liposomes adsorbed on TiO(2) microspheres were intact and there was no drug leakage. The study proposed pseudo-first and the pseudo-second order equations to facilitate the adsorption of liposomal drugs with TiO(2) microspheres. The proposed strategy supports the pharmacokinetic study of docetaxel liposomes in rats. CONCLUSION: TiO(2) extraction method was stable, reproducible, and reliable for quantitation of encapsulated docetaxel. Because of versatility of lipids, it is expected to a universal bioanalysis method for the pharmacokinetic study of liposomes.
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spelling pubmed-100299722023-03-22 A Novel Strategy for Liposomal Drug Separation in Plasma by TiO(2) Microspheres and Application in Pharmacokinetics Yu, Yue-yang Yuan, Mei Qin, Wei-jie Bai, Hai-hong Liu, Hong-zhuo Che, Jin-jing Int J Nanomedicine Original Research PURPOSE: Liposomes are nano-scale materials with a biofilm-like structure. They have excellent biocompatibility and are increasingly useful in drug delivery systems. However, the in vivo fate of liposomal drugs is still unclear because existing bioanalytical methods for quantitation of total and liposomal-encapsulated drugs have limits. A novel strategy for liposomal-encapsulated drug separation from plasma was developed via the specific coordinate binding interaction of TiO(2) microspheres with the phosphate groups of liposomes. METHODS: Liposomal-encapsulated docetaxel was separated from plasma by TiO(2) microspheres and analyzed by the UPLC-MS/MS method. The amount of TiO(2), pH of the dilutions, plasma dilution factors and incubation time were optimized to improve extraction recovery. The characterization of the adsorption of liposome-encapsulated drugs by TiO(2) microspheres was observed by electron microscopy. For understanding the mechanism, pseudo-first and the pseudo-second order equations were proposed for the adsorption process. The study fully validated the method for quantitation of liposomal-encapsulated in plasma and the method was applied to the pharmacokinetic study of docetaxel liposomes. RESULTS: The encapsulated docetaxel had a concentration range of 15–4000 ng/mL from the plasma sample using a TiO(2) extraction method. Successful method validation proved the method was sensitive, selective and stable, and was suitable for quantitation of docetaxel liposomes in plasma samples. Extraction recovery of this method was higher than that of SPE method. As shown in electron microscopy, the liposomes adsorbed on TiO(2) microspheres were intact and there was no drug leakage. The study proposed pseudo-first and the pseudo-second order equations to facilitate the adsorption of liposomal drugs with TiO(2) microspheres. The proposed strategy supports the pharmacokinetic study of docetaxel liposomes in rats. CONCLUSION: TiO(2) extraction method was stable, reproducible, and reliable for quantitation of encapsulated docetaxel. Because of versatility of lipids, it is expected to a universal bioanalysis method for the pharmacokinetic study of liposomes. Dove 2023-03-17 /pmc/articles/PMC10029972/ /pubmed/36960125 http://dx.doi.org/10.2147/IJN.S396746 Text en © 2023 Yu et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Yu, Yue-yang
Yuan, Mei
Qin, Wei-jie
Bai, Hai-hong
Liu, Hong-zhuo
Che, Jin-jing
A Novel Strategy for Liposomal Drug Separation in Plasma by TiO(2) Microspheres and Application in Pharmacokinetics
title A Novel Strategy for Liposomal Drug Separation in Plasma by TiO(2) Microspheres and Application in Pharmacokinetics
title_full A Novel Strategy for Liposomal Drug Separation in Plasma by TiO(2) Microspheres and Application in Pharmacokinetics
title_fullStr A Novel Strategy for Liposomal Drug Separation in Plasma by TiO(2) Microspheres and Application in Pharmacokinetics
title_full_unstemmed A Novel Strategy for Liposomal Drug Separation in Plasma by TiO(2) Microspheres and Application in Pharmacokinetics
title_short A Novel Strategy for Liposomal Drug Separation in Plasma by TiO(2) Microspheres and Application in Pharmacokinetics
title_sort novel strategy for liposomal drug separation in plasma by tio(2) microspheres and application in pharmacokinetics
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10029972/
https://www.ncbi.nlm.nih.gov/pubmed/36960125
http://dx.doi.org/10.2147/IJN.S396746
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