Cargando…

A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution

Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which generally lack the single-cell resolution required for reli...

Descripción completa

Detalles Bibliográficos
Autores principales: Meissner, Geoffrey W, Nern, Aljoscha, Dorman, Zachary, DePasquale, Gina M, Forster, Kaitlyn, Gibney, Theresa, Hausenfluck, Joanna H, He, Yisheng, Iyer, Nirmala A, Jeter, Jennifer, Johnson, Lauren, Johnston, Rebecca M, Lee, Kelley, Melton, Brian, Yarbrough, Brianna, Zugates, Christopher T, Clements, Jody, Goina, Cristian, Otsuna, Hideo, Rokicki, Konrad, Svirskas, Robert R, Aso, Yoshinori, Card, Gwyneth M, Dickson, Barry J, Ehrhardt, Erica, Goldammer, Jens, Ito, Masayoshi, Kainmueller, Dagmar, Korff, Wyatt, Mais, Lisa, Minegishi, Ryo, Namiki, Shigehiro, Rubin, Gerald M, Sterne, Gabriella R, Wolff, Tanya, Malkesman, Oz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10030108/
https://www.ncbi.nlm.nih.gov/pubmed/36820523
http://dx.doi.org/10.7554/eLife.80660
_version_ 1784910286151483392
author Meissner, Geoffrey W
Nern, Aljoscha
Dorman, Zachary
DePasquale, Gina M
Forster, Kaitlyn
Gibney, Theresa
Hausenfluck, Joanna H
He, Yisheng
Iyer, Nirmala A
Jeter, Jennifer
Johnson, Lauren
Johnston, Rebecca M
Lee, Kelley
Melton, Brian
Yarbrough, Brianna
Zugates, Christopher T
Clements, Jody
Goina, Cristian
Otsuna, Hideo
Rokicki, Konrad
Svirskas, Robert R
Aso, Yoshinori
Card, Gwyneth M
Dickson, Barry J
Ehrhardt, Erica
Goldammer, Jens
Ito, Masayoshi
Kainmueller, Dagmar
Korff, Wyatt
Mais, Lisa
Minegishi, Ryo
Namiki, Shigehiro
Rubin, Gerald M
Sterne, Gabriella R
Wolff, Tanya
Malkesman, Oz
author_facet Meissner, Geoffrey W
Nern, Aljoscha
Dorman, Zachary
DePasquale, Gina M
Forster, Kaitlyn
Gibney, Theresa
Hausenfluck, Joanna H
He, Yisheng
Iyer, Nirmala A
Jeter, Jennifer
Johnson, Lauren
Johnston, Rebecca M
Lee, Kelley
Melton, Brian
Yarbrough, Brianna
Zugates, Christopher T
Clements, Jody
Goina, Cristian
Otsuna, Hideo
Rokicki, Konrad
Svirskas, Robert R
Aso, Yoshinori
Card, Gwyneth M
Dickson, Barry J
Ehrhardt, Erica
Goldammer, Jens
Ito, Masayoshi
Kainmueller, Dagmar
Korff, Wyatt
Mais, Lisa
Minegishi, Ryo
Namiki, Shigehiro
Rubin, Gerald M
Sterne, Gabriella R
Wolff, Tanya
Malkesman, Oz
author_sort Meissner, Geoffrey W
collection PubMed
description Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here, we use stochastic GAL4 labeling with the MultiColor FlpOut approach to generate cellular resolution confocal images at large scale. We are releasing aligned images of 74,000 such adult central nervous systems. An anticipated use of this resource is to bridge the gap between neurons identified by electron or light microscopy. Identifying individual neurons that make up each GAL4 expression pattern improves the prediction of split-GAL4 combinations targeting particular neurons. To this end, we have made the images searchable on the NeuronBridge website. We demonstrate the potential of NeuronBridge to rapidly and effectively identify neuron matches based on morphology across imaging modalities and datasets.
format Online
Article
Text
id pubmed-10030108
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher eLife Sciences Publications, Ltd
record_format MEDLINE/PubMed
spelling pubmed-100301082023-03-22 A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution Meissner, Geoffrey W Nern, Aljoscha Dorman, Zachary DePasquale, Gina M Forster, Kaitlyn Gibney, Theresa Hausenfluck, Joanna H He, Yisheng Iyer, Nirmala A Jeter, Jennifer Johnson, Lauren Johnston, Rebecca M Lee, Kelley Melton, Brian Yarbrough, Brianna Zugates, Christopher T Clements, Jody Goina, Cristian Otsuna, Hideo Rokicki, Konrad Svirskas, Robert R Aso, Yoshinori Card, Gwyneth M Dickson, Barry J Ehrhardt, Erica Goldammer, Jens Ito, Masayoshi Kainmueller, Dagmar Korff, Wyatt Mais, Lisa Minegishi, Ryo Namiki, Shigehiro Rubin, Gerald M Sterne, Gabriella R Wolff, Tanya Malkesman, Oz eLife Neuroscience Precise, repeatable genetic access to specific neurons via GAL4/UAS and related methods is a key advantage of Drosophila neuroscience. Neuronal targeting is typically documented using light microscopy of full GAL4 expression patterns, which generally lack the single-cell resolution required for reliable cell type identification. Here, we use stochastic GAL4 labeling with the MultiColor FlpOut approach to generate cellular resolution confocal images at large scale. We are releasing aligned images of 74,000 such adult central nervous systems. An anticipated use of this resource is to bridge the gap between neurons identified by electron or light microscopy. Identifying individual neurons that make up each GAL4 expression pattern improves the prediction of split-GAL4 combinations targeting particular neurons. To this end, we have made the images searchable on the NeuronBridge website. We demonstrate the potential of NeuronBridge to rapidly and effectively identify neuron matches based on morphology across imaging modalities and datasets. eLife Sciences Publications, Ltd 2023-02-23 /pmc/articles/PMC10030108/ /pubmed/36820523 http://dx.doi.org/10.7554/eLife.80660 Text en © 2023, Meissner et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Meissner, Geoffrey W
Nern, Aljoscha
Dorman, Zachary
DePasquale, Gina M
Forster, Kaitlyn
Gibney, Theresa
Hausenfluck, Joanna H
He, Yisheng
Iyer, Nirmala A
Jeter, Jennifer
Johnson, Lauren
Johnston, Rebecca M
Lee, Kelley
Melton, Brian
Yarbrough, Brianna
Zugates, Christopher T
Clements, Jody
Goina, Cristian
Otsuna, Hideo
Rokicki, Konrad
Svirskas, Robert R
Aso, Yoshinori
Card, Gwyneth M
Dickson, Barry J
Ehrhardt, Erica
Goldammer, Jens
Ito, Masayoshi
Kainmueller, Dagmar
Korff, Wyatt
Mais, Lisa
Minegishi, Ryo
Namiki, Shigehiro
Rubin, Gerald M
Sterne, Gabriella R
Wolff, Tanya
Malkesman, Oz
A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution
title A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution
title_full A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution
title_fullStr A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution
title_full_unstemmed A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution
title_short A searchable image resource of Drosophila GAL4 driver expression patterns with single neuron resolution
title_sort searchable image resource of drosophila gal4 driver expression patterns with single neuron resolution
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10030108/
https://www.ncbi.nlm.nih.gov/pubmed/36820523
http://dx.doi.org/10.7554/eLife.80660
work_keys_str_mv AT meissnergeoffreyw asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT nernaljoscha asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT dormanzachary asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT depasqualeginam asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT forsterkaitlyn asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT gibneytheresa asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT hausenfluckjoannah asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT heyisheng asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT iyernirmalaa asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT jeterjennifer asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT johnsonlauren asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT johnstonrebeccam asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT leekelley asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT meltonbrian asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT yarbroughbrianna asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT zugateschristophert asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT clementsjody asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT goinacristian asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT otsunahideo asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT rokickikonrad asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT svirskasrobertr asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT asoyoshinori asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT cardgwynethm asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT dicksonbarryj asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT ehrhardterica asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT goldammerjens asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT itomasayoshi asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT kainmuellerdagmar asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT korffwyatt asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT maislisa asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT minegishiryo asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT namikishigehiro asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT rubingeraldm asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT sternegabriellar asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT wolfftanya asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT malkesmanoz asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT asearchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT meissnergeoffreyw searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT nernaljoscha searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT dormanzachary searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT depasqualeginam searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT forsterkaitlyn searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT gibneytheresa searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT hausenfluckjoannah searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT heyisheng searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT iyernirmalaa searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT jeterjennifer searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT johnsonlauren searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT johnstonrebeccam searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT leekelley searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT meltonbrian searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT yarbroughbrianna searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT zugateschristophert searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT clementsjody searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT goinacristian searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT otsunahideo searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT rokickikonrad searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT svirskasrobertr searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT asoyoshinori searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT cardgwynethm searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT dicksonbarryj searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT ehrhardterica searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT goldammerjens searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT itomasayoshi searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT kainmuellerdagmar searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT korffwyatt searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT maislisa searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT minegishiryo searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT namikishigehiro searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT rubingeraldm searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT sternegabriellar searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT wolfftanya searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT malkesmanoz searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution
AT searchableimageresourceofdrosophilagal4driverexpressionpatternswithsingleneuronresolution