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A Comparative Evaluation of Antibacterial Efficacy of Moringa oleifera Leaf Extract, Octenidine Dihydrochloride, and Sodium Hypochlorite as Intracanal Irrigants against Enterococcus faecalis: An In Vitro Study

OBJECTIVES: The aim of this study is to investigate and compare the microbial efficacy of Moringa oleifera leaf extract, octenidine dihydrochloride (OCT), NaOCl, and their combinations as intracanal irrigants against Enterococcus faecalis. MATERIALS AND METHODS: Sixty single-rooted mandibular premol...

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Autores principales: Alharbi, Afrah M., Alharbi, Tahani M., Alqahtani, Mashael S., Elfasakhany, Fathy M., Afifi, Ibtesam K., Rajeh, Mona T., Fattouh, Mohamed, Kenawi, Laila Mohamed Mohamed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10030228/
https://www.ncbi.nlm.nih.gov/pubmed/36960331
http://dx.doi.org/10.1155/2023/7690497
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author Alharbi, Afrah M.
Alharbi, Tahani M.
Alqahtani, Mashael S.
Elfasakhany, Fathy M.
Afifi, Ibtesam K.
Rajeh, Mona T.
Fattouh, Mohamed
Kenawi, Laila Mohamed Mohamed
author_facet Alharbi, Afrah M.
Alharbi, Tahani M.
Alqahtani, Mashael S.
Elfasakhany, Fathy M.
Afifi, Ibtesam K.
Rajeh, Mona T.
Fattouh, Mohamed
Kenawi, Laila Mohamed Mohamed
author_sort Alharbi, Afrah M.
collection PubMed
description OBJECTIVES: The aim of this study is to investigate and compare the microbial efficacy of Moringa oleifera leaf extract, octenidine dihydrochloride (OCT), NaOCl, and their combinations as intracanal irrigants against Enterococcus faecalis. MATERIALS AND METHODS: Sixty single-rooted mandibular premolars were decoronated followed by root canal preparation. Each root specimen was autoclaved, inoculated with E. faecalis, and incubated at 37°C for 48 hr. Then, the specimens were divided into six groups based on the irrigation solution used: 2.5% NaOCl (Group 1), 0.1% OCT (Group 2), M. oleifera leaves extract (Group 3), a combination of M. oleifera extract and 1.25% NaOCl (Group 4), a combination of M. oleifera extract and OCT (Group 5) and normal saline (Group 6). Microbial samples were taken from each root canal before (S1) and after (S2) irrigation and the bacterial viability was assessed using colony-forming units (CFU) on bile esculin agar plates. RESULTS: Comparing the number of CFU/ml before and after irrigation showed a significant reduction (P < 0.001) in all studied groups. Comparison between the CFU/ml after irrigation by NaOCl and each of the combination groups showed a significant difference. CONCLUSION: M. oleifera leaves extract and 0.1% OCT solutions have antibacterial effect against E. faecalis comparable to 2.5% NaOCl and might be used as root canal irrigants. The combination groups showed better antimicrobial activities than individual irrigants. However, further studies are required to investigate the biocompatibility and possible toxic effects of the tested irrigants.
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spelling pubmed-100302282023-03-22 A Comparative Evaluation of Antibacterial Efficacy of Moringa oleifera Leaf Extract, Octenidine Dihydrochloride, and Sodium Hypochlorite as Intracanal Irrigants against Enterococcus faecalis: An In Vitro Study Alharbi, Afrah M. Alharbi, Tahani M. Alqahtani, Mashael S. Elfasakhany, Fathy M. Afifi, Ibtesam K. Rajeh, Mona T. Fattouh, Mohamed Kenawi, Laila Mohamed Mohamed Int J Dent Research Article OBJECTIVES: The aim of this study is to investigate and compare the microbial efficacy of Moringa oleifera leaf extract, octenidine dihydrochloride (OCT), NaOCl, and their combinations as intracanal irrigants against Enterococcus faecalis. MATERIALS AND METHODS: Sixty single-rooted mandibular premolars were decoronated followed by root canal preparation. Each root specimen was autoclaved, inoculated with E. faecalis, and incubated at 37°C for 48 hr. Then, the specimens were divided into six groups based on the irrigation solution used: 2.5% NaOCl (Group 1), 0.1% OCT (Group 2), M. oleifera leaves extract (Group 3), a combination of M. oleifera extract and 1.25% NaOCl (Group 4), a combination of M. oleifera extract and OCT (Group 5) and normal saline (Group 6). Microbial samples were taken from each root canal before (S1) and after (S2) irrigation and the bacterial viability was assessed using colony-forming units (CFU) on bile esculin agar plates. RESULTS: Comparing the number of CFU/ml before and after irrigation showed a significant reduction (P < 0.001) in all studied groups. Comparison between the CFU/ml after irrigation by NaOCl and each of the combination groups showed a significant difference. CONCLUSION: M. oleifera leaves extract and 0.1% OCT solutions have antibacterial effect against E. faecalis comparable to 2.5% NaOCl and might be used as root canal irrigants. The combination groups showed better antimicrobial activities than individual irrigants. However, further studies are required to investigate the biocompatibility and possible toxic effects of the tested irrigants. Hindawi 2023-03-14 /pmc/articles/PMC10030228/ /pubmed/36960331 http://dx.doi.org/10.1155/2023/7690497 Text en Copyright © 2023 Afrah M. Alharbi et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Alharbi, Afrah M.
Alharbi, Tahani M.
Alqahtani, Mashael S.
Elfasakhany, Fathy M.
Afifi, Ibtesam K.
Rajeh, Mona T.
Fattouh, Mohamed
Kenawi, Laila Mohamed Mohamed
A Comparative Evaluation of Antibacterial Efficacy of Moringa oleifera Leaf Extract, Octenidine Dihydrochloride, and Sodium Hypochlorite as Intracanal Irrigants against Enterococcus faecalis: An In Vitro Study
title A Comparative Evaluation of Antibacterial Efficacy of Moringa oleifera Leaf Extract, Octenidine Dihydrochloride, and Sodium Hypochlorite as Intracanal Irrigants against Enterococcus faecalis: An In Vitro Study
title_full A Comparative Evaluation of Antibacterial Efficacy of Moringa oleifera Leaf Extract, Octenidine Dihydrochloride, and Sodium Hypochlorite as Intracanal Irrigants against Enterococcus faecalis: An In Vitro Study
title_fullStr A Comparative Evaluation of Antibacterial Efficacy of Moringa oleifera Leaf Extract, Octenidine Dihydrochloride, and Sodium Hypochlorite as Intracanal Irrigants against Enterococcus faecalis: An In Vitro Study
title_full_unstemmed A Comparative Evaluation of Antibacterial Efficacy of Moringa oleifera Leaf Extract, Octenidine Dihydrochloride, and Sodium Hypochlorite as Intracanal Irrigants against Enterococcus faecalis: An In Vitro Study
title_short A Comparative Evaluation of Antibacterial Efficacy of Moringa oleifera Leaf Extract, Octenidine Dihydrochloride, and Sodium Hypochlorite as Intracanal Irrigants against Enterococcus faecalis: An In Vitro Study
title_sort comparative evaluation of antibacterial efficacy of moringa oleifera leaf extract, octenidine dihydrochloride, and sodium hypochlorite as intracanal irrigants against enterococcus faecalis: an in vitro study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10030228/
https://www.ncbi.nlm.nih.gov/pubmed/36960331
http://dx.doi.org/10.1155/2023/7690497
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