Cargando…
EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii
The control of coccidiosis, causing huge economic losses in the poultry industry, is facing the stagnation of the development of new drugs and the emergence of drug resistance. Thus, the priority for coccidiosis control is to decipher the effect mechanisms and resistance mechanisms of anticoccidial...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031133/ https://www.ncbi.nlm.nih.gov/pubmed/36968087 http://dx.doi.org/10.1016/j.isci.2023.106334 |
_version_ | 1784910537645096960 |
---|---|
author | Sun, Pei Zhang, Yuanyuan Wang, Chaoyue Hu, Dandan Liu, Jie Chen, Linlin Shi, Fangyun Tang, Xinming Hao, Zhenkai Suo, Jingxia Yu, Yonglan Suo, Xun Liu, Xianyong |
author_facet | Sun, Pei Zhang, Yuanyuan Wang, Chaoyue Hu, Dandan Liu, Jie Chen, Linlin Shi, Fangyun Tang, Xinming Hao, Zhenkai Suo, Jingxia Yu, Yonglan Suo, Xun Liu, Xianyong |
author_sort | Sun, Pei |
collection | PubMed |
description | The control of coccidiosis, causing huge economic losses in the poultry industry, is facing the stagnation of the development of new drugs and the emergence of drug resistance. Thus, the priority for coccidiosis control is to decipher the effect mechanisms and resistance mechanisms of anticoccidial drugs. In this study, we mined and validated a molecular marker for halofuginone resistance in Eimeria tenella through forward and reverse genetic approaches. We screened whole-genome sequencing data and detected point mutations in the ETH2_1020900 gene (encoding prolyl-tRNA synthetase, PRS). Then, we introduced this mutated gene into E. tenella and Toxoplasma gondii and validated that overexpression of this mutated gene confers resistance to halofuginone in vivo and in vitro. These results together show that mutations A1852G and A1854G on the ETH2_1020900 gene are pivotal to halofuginone resistance in E. tenella, encouraging the exploration of mechanisms of drug resistance against other anticoccidial drugs in eimerian parasites. |
format | Online Article Text |
id | pubmed-10031133 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-100311332023-03-23 EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii Sun, Pei Zhang, Yuanyuan Wang, Chaoyue Hu, Dandan Liu, Jie Chen, Linlin Shi, Fangyun Tang, Xinming Hao, Zhenkai Suo, Jingxia Yu, Yonglan Suo, Xun Liu, Xianyong iScience Article The control of coccidiosis, causing huge economic losses in the poultry industry, is facing the stagnation of the development of new drugs and the emergence of drug resistance. Thus, the priority for coccidiosis control is to decipher the effect mechanisms and resistance mechanisms of anticoccidial drugs. In this study, we mined and validated a molecular marker for halofuginone resistance in Eimeria tenella through forward and reverse genetic approaches. We screened whole-genome sequencing data and detected point mutations in the ETH2_1020900 gene (encoding prolyl-tRNA synthetase, PRS). Then, we introduced this mutated gene into E. tenella and Toxoplasma gondii and validated that overexpression of this mutated gene confers resistance to halofuginone in vivo and in vitro. These results together show that mutations A1852G and A1854G on the ETH2_1020900 gene are pivotal to halofuginone resistance in E. tenella, encouraging the exploration of mechanisms of drug resistance against other anticoccidial drugs in eimerian parasites. Elsevier 2023-03-04 /pmc/articles/PMC10031133/ /pubmed/36968087 http://dx.doi.org/10.1016/j.isci.2023.106334 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Sun, Pei Zhang, Yuanyuan Wang, Chaoyue Hu, Dandan Liu, Jie Chen, Linlin Shi, Fangyun Tang, Xinming Hao, Zhenkai Suo, Jingxia Yu, Yonglan Suo, Xun Liu, Xianyong EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii |
title | EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii |
title_full | EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii |
title_fullStr | EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii |
title_full_unstemmed | EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii |
title_short | EtcPRS(Mut) as a molecular marker of halofuginone resistance in Eimeria tenella and Toxoplasma gondii |
title_sort | etcprs(mut) as a molecular marker of halofuginone resistance in eimeria tenella and toxoplasma gondii |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031133/ https://www.ncbi.nlm.nih.gov/pubmed/36968087 http://dx.doi.org/10.1016/j.isci.2023.106334 |
work_keys_str_mv | AT sunpei etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT zhangyuanyuan etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT wangchaoyue etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT hudandan etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT liujie etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT chenlinlin etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT shifangyun etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT tangxinming etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT haozhenkai etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT suojingxia etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT yuyonglan etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT suoxun etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii AT liuxianyong etcprsmutasamolecularmarkerofhalofuginoneresistanceineimeriatenellaandtoxoplasmagondii |