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MAC-1 marks a quiescent and functionally superior HSC subset during regeneration

Mouse hematopoietic stem cells (HSCs) have been extensively defined both molecularly and functionally at steady state, while regenerative stress induces immunophenotypical changes that limit high purity isolation and analysis. It is therefore important to identify markers that specifically label act...

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Autores principales: Rydström, Anna, Mansell, Els, Sigurdsson, Valgardur, Sjöberg, Julia, Soneji, Shamit, Miharada, Kenichi, Larsson, Jonas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031298/
https://www.ncbi.nlm.nih.gov/pubmed/36868231
http://dx.doi.org/10.1016/j.stemcr.2023.01.014
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author Rydström, Anna
Mansell, Els
Sigurdsson, Valgardur
Sjöberg, Julia
Soneji, Shamit
Miharada, Kenichi
Larsson, Jonas
author_facet Rydström, Anna
Mansell, Els
Sigurdsson, Valgardur
Sjöberg, Julia
Soneji, Shamit
Miharada, Kenichi
Larsson, Jonas
author_sort Rydström, Anna
collection PubMed
description Mouse hematopoietic stem cells (HSCs) have been extensively defined both molecularly and functionally at steady state, while regenerative stress induces immunophenotypical changes that limit high purity isolation and analysis. It is therefore important to identify markers that specifically label activated HSCs to gain further knowledge about their molecular and functional properties. Here, we assessed the expression of macrophage-1 antigen (MAC-1) on HSCs during regeneration following transplantation and observed a transient increase in MAC-1 expression during the early reconstitution phase. Serial transplantation experiments demonstrated that reconstitution potential was highly enriched in the MAC-1(+) portion of the HSC pool. Moreover, in contrast to previous reports, we found that MAC-1 expression inversely correlates with cell cycling, and global transcriptome analysis showed that regenerating MAC-1(+) HSCs share molecular features with stem cells with low mitotic history. Taken together, our results suggest that MAC-1 expression marks predominantly quiescent and functionally superior HSCs during early regeneration.
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spelling pubmed-100312982023-03-23 MAC-1 marks a quiescent and functionally superior HSC subset during regeneration Rydström, Anna Mansell, Els Sigurdsson, Valgardur Sjöberg, Julia Soneji, Shamit Miharada, Kenichi Larsson, Jonas Stem Cell Reports Article Mouse hematopoietic stem cells (HSCs) have been extensively defined both molecularly and functionally at steady state, while regenerative stress induces immunophenotypical changes that limit high purity isolation and analysis. It is therefore important to identify markers that specifically label activated HSCs to gain further knowledge about their molecular and functional properties. Here, we assessed the expression of macrophage-1 antigen (MAC-1) on HSCs during regeneration following transplantation and observed a transient increase in MAC-1 expression during the early reconstitution phase. Serial transplantation experiments demonstrated that reconstitution potential was highly enriched in the MAC-1(+) portion of the HSC pool. Moreover, in contrast to previous reports, we found that MAC-1 expression inversely correlates with cell cycling, and global transcriptome analysis showed that regenerating MAC-1(+) HSCs share molecular features with stem cells with low mitotic history. Taken together, our results suggest that MAC-1 expression marks predominantly quiescent and functionally superior HSCs during early regeneration. Elsevier 2023-03-02 /pmc/articles/PMC10031298/ /pubmed/36868231 http://dx.doi.org/10.1016/j.stemcr.2023.01.014 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Rydström, Anna
Mansell, Els
Sigurdsson, Valgardur
Sjöberg, Julia
Soneji, Shamit
Miharada, Kenichi
Larsson, Jonas
MAC-1 marks a quiescent and functionally superior HSC subset during regeneration
title MAC-1 marks a quiescent and functionally superior HSC subset during regeneration
title_full MAC-1 marks a quiescent and functionally superior HSC subset during regeneration
title_fullStr MAC-1 marks a quiescent and functionally superior HSC subset during regeneration
title_full_unstemmed MAC-1 marks a quiescent and functionally superior HSC subset during regeneration
title_short MAC-1 marks a quiescent and functionally superior HSC subset during regeneration
title_sort mac-1 marks a quiescent and functionally superior hsc subset during regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031298/
https://www.ncbi.nlm.nih.gov/pubmed/36868231
http://dx.doi.org/10.1016/j.stemcr.2023.01.014
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