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H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra
The evolution of the first body axis in the animal kingdom and its extensive ability to regenerate makes Hydra, a Cnidarian, an excellent model system for understanding the underlying epigenetic mechanisms. We identify that monomethyltransferase SETD8 is critical for regeneration in Hydra because of...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031314/ https://www.ncbi.nlm.nih.gov/pubmed/36944423 http://dx.doi.org/10.26508/lsa.202201619 |
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author | Gungi, Akhila Saha, Shagnik Pal, Mrinmoy Galande, Sanjeev |
author_facet | Gungi, Akhila Saha, Shagnik Pal, Mrinmoy Galande, Sanjeev |
author_sort | Gungi, Akhila |
collection | PubMed |
description | The evolution of the first body axis in the animal kingdom and its extensive ability to regenerate makes Hydra, a Cnidarian, an excellent model system for understanding the underlying epigenetic mechanisms. We identify that monomethyltransferase SETD8 is critical for regeneration in Hydra because of its conserved interaction with β-catenin to fine-tune the associated gene regulatory network. Inhibition of SETD8 activity abolishes head and foot regeneration in Hydra. Furthermore, we show that H4K20me1, the histone mark imparted by SETD8, colocalizes with the transcriptional activation machinery locally at the β-catenin-bound TCF/LEF-binding sites on the promoters of head-associated genes, marking an epigenetic activation mode. In contrast, genome-wide analysis of the H4K20me1 occupancy revealed a negative correlation with transcriptional activation. We propose that H4K20me1 acts as a general repressive histone mark in Cnidaria and describe its dichotomous role in transcriptional regulation in Hydra. |
format | Online Article Text |
id | pubmed-10031314 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Life Science Alliance LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-100313142023-03-23 H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra Gungi, Akhila Saha, Shagnik Pal, Mrinmoy Galande, Sanjeev Life Sci Alliance Research Articles The evolution of the first body axis in the animal kingdom and its extensive ability to regenerate makes Hydra, a Cnidarian, an excellent model system for understanding the underlying epigenetic mechanisms. We identify that monomethyltransferase SETD8 is critical for regeneration in Hydra because of its conserved interaction with β-catenin to fine-tune the associated gene regulatory network. Inhibition of SETD8 activity abolishes head and foot regeneration in Hydra. Furthermore, we show that H4K20me1, the histone mark imparted by SETD8, colocalizes with the transcriptional activation machinery locally at the β-catenin-bound TCF/LEF-binding sites on the promoters of head-associated genes, marking an epigenetic activation mode. In contrast, genome-wide analysis of the H4K20me1 occupancy revealed a negative correlation with transcriptional activation. We propose that H4K20me1 acts as a general repressive histone mark in Cnidaria and describe its dichotomous role in transcriptional regulation in Hydra. Life Science Alliance LLC 2023-03-21 /pmc/articles/PMC10031314/ /pubmed/36944423 http://dx.doi.org/10.26508/lsa.202201619 Text en © 2023 Gungi et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Research Articles Gungi, Akhila Saha, Shagnik Pal, Mrinmoy Galande, Sanjeev H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra |
title | H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra |
title_full | H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra |
title_fullStr | H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra |
title_full_unstemmed | H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra |
title_short | H4K20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in Hydra |
title_sort | h4k20me1 plays a dual role in transcriptional regulation of regeneration and axis patterning in hydra |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031314/ https://www.ncbi.nlm.nih.gov/pubmed/36944423 http://dx.doi.org/10.26508/lsa.202201619 |
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