Separating Inner and Outer Membranes of Escherichia coli by EDTA-free Sucrose Gradient Centrifugation

The envelope of Gram-negative bacteria consists of an outer membrane (OM), a peptidoglycan cell wall, and an inner membrane (IM). The OM and IM have different components of proteins and lipids. Separating the IM and OM is a basic biochemical procedure to further study lipids and membrane proteins in...

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Detalles Bibliográficos
Autores principales: Shu, Sheng, Mi, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bio-Protocol 2023
Materias:
Methods Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031520/
https://www.ncbi.nlm.nih.gov/pubmed/36968434
http://dx.doi.org/10.21769/BioProtoc.4638

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031520/
https://www.ncbi.nlm.nih.gov/pubmed/36968434
http://dx.doi.org/10.21769/BioProtoc.4638

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