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A Miniature Sucrose Gradient for Polysome Profiling
Polysome profiling by sucrose density gradient centrifugation is commonly used to study the overall degree of translation (messenger RNA to protein synthesis). Traditionally, the method begins with synthesis of a 5–10 mL sucrose gradient onto which 0.5–1 mL of cell extract is layered and centrifuged...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Bio-Protocol
2023
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031523/ https://www.ncbi.nlm.nih.gov/pubmed/36968436 http://dx.doi.org/10.21769/BioProtoc.4622 |