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Identification of lncRNA and mRNA regulatory networks associated with gastric cancer progression

Gastric cancer is a tumor type characterized by lymph node metastasis and the invasion of local tissues. There is thus a critical need to clarify the molecular mechanisms governing gastric cancer onset and progression to guide the treatment of this disease. Long non-coding RNAs and mRNA expression p...

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Autores principales: Sun, Ke-kang, Zu, Chao, Wu, Xiao-yang, Wang, Qing-hua, Hua, Peng, Zhang, Yi-fang, Shen, Xiao-jun, Wu, Yong-you
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031762/
https://www.ncbi.nlm.nih.gov/pubmed/36969001
http://dx.doi.org/10.3389/fonc.2023.1140460
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author Sun, Ke-kang
Zu, Chao
Wu, Xiao-yang
Wang, Qing-hua
Hua, Peng
Zhang, Yi-fang
Shen, Xiao-jun
Wu, Yong-you
author_facet Sun, Ke-kang
Zu, Chao
Wu, Xiao-yang
Wang, Qing-hua
Hua, Peng
Zhang, Yi-fang
Shen, Xiao-jun
Wu, Yong-you
author_sort Sun, Ke-kang
collection PubMed
description Gastric cancer is a tumor type characterized by lymph node metastasis and the invasion of local tissues. There is thus a critical need to clarify the molecular mechanisms governing gastric cancer onset and progression to guide the treatment of this disease. Long non-coding RNAs and mRNA expression profiles associated with early and local advanced gastric cancer were examined through microarray analyses, with GO and KEGG analyses being employed as a means of exploring the functional roles of those long non-coding RNAs and mRNAs that were differentially expressed in gastric cancer. In total, 1005 and 1831 lncRNAs and mRNAs, respectively, were found to be differentially expressed between early and local advanced gastric cancer. GO and KEGG analyses revealed several pathways and processes that were dysregulated, including the RNA transport, ECM-receptor interaction, and mRNA splicing pathways. In co-expression networks, E2F1, E2F4, and STAT2 were identified as key transcriptional regulators of these processes. Moreover, thrombospondin-2 was confirmed as being expressed at high levels in more advanced gastric cancer by both the GEO and TCGA databases. RNA-sequencing analyses of SGC-790 cells transfected to express thrombospondin-2 further revealed this gene to enhance NF-kB and TNF pathway signaling activity. These results offer insight into gastric cancer-related regulatory networks and suggest thrombospondin-2 to be an important oncogene that drives the progression of this deadly cancer type.
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spelling pubmed-100317622023-03-23 Identification of lncRNA and mRNA regulatory networks associated with gastric cancer progression Sun, Ke-kang Zu, Chao Wu, Xiao-yang Wang, Qing-hua Hua, Peng Zhang, Yi-fang Shen, Xiao-jun Wu, Yong-you Front Oncol Oncology Gastric cancer is a tumor type characterized by lymph node metastasis and the invasion of local tissues. There is thus a critical need to clarify the molecular mechanisms governing gastric cancer onset and progression to guide the treatment of this disease. Long non-coding RNAs and mRNA expression profiles associated with early and local advanced gastric cancer were examined through microarray analyses, with GO and KEGG analyses being employed as a means of exploring the functional roles of those long non-coding RNAs and mRNAs that were differentially expressed in gastric cancer. In total, 1005 and 1831 lncRNAs and mRNAs, respectively, were found to be differentially expressed between early and local advanced gastric cancer. GO and KEGG analyses revealed several pathways and processes that were dysregulated, including the RNA transport, ECM-receptor interaction, and mRNA splicing pathways. In co-expression networks, E2F1, E2F4, and STAT2 were identified as key transcriptional regulators of these processes. Moreover, thrombospondin-2 was confirmed as being expressed at high levels in more advanced gastric cancer by both the GEO and TCGA databases. RNA-sequencing analyses of SGC-790 cells transfected to express thrombospondin-2 further revealed this gene to enhance NF-kB and TNF pathway signaling activity. These results offer insight into gastric cancer-related regulatory networks and suggest thrombospondin-2 to be an important oncogene that drives the progression of this deadly cancer type. Frontiers Media S.A. 2023-03-06 /pmc/articles/PMC10031762/ /pubmed/36969001 http://dx.doi.org/10.3389/fonc.2023.1140460 Text en Copyright © 2023 Sun, Zu, Wu, Wang, Hua, Zhang, Shen and Wu https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Sun, Ke-kang
Zu, Chao
Wu, Xiao-yang
Wang, Qing-hua
Hua, Peng
Zhang, Yi-fang
Shen, Xiao-jun
Wu, Yong-you
Identification of lncRNA and mRNA regulatory networks associated with gastric cancer progression
title Identification of lncRNA and mRNA regulatory networks associated with gastric cancer progression
title_full Identification of lncRNA and mRNA regulatory networks associated with gastric cancer progression
title_fullStr Identification of lncRNA and mRNA regulatory networks associated with gastric cancer progression
title_full_unstemmed Identification of lncRNA and mRNA regulatory networks associated with gastric cancer progression
title_short Identification of lncRNA and mRNA regulatory networks associated with gastric cancer progression
title_sort identification of lncrna and mrna regulatory networks associated with gastric cancer progression
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031762/
https://www.ncbi.nlm.nih.gov/pubmed/36969001
http://dx.doi.org/10.3389/fonc.2023.1140460
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