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Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials

BACKGROUND: Zoonotic livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is widely spread in pig herds in many countries. However, the knowledge regarding the survival of LA-MRSA in the pig farm environment is currently limited. The aim of this study was to assess the survival...

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Autores principales: Tuominen, Krista, Frosth, Sara, Pedersen, Karl, Rosendal, Thomas, Sternberg Lewerin, Susanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031894/
https://www.ncbi.nlm.nih.gov/pubmed/36944983
http://dx.doi.org/10.1186/s13028-023-00676-z
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author Tuominen, Krista
Frosth, Sara
Pedersen, Karl
Rosendal, Thomas
Sternberg Lewerin, Susanna
author_facet Tuominen, Krista
Frosth, Sara
Pedersen, Karl
Rosendal, Thomas
Sternberg Lewerin, Susanna
author_sort Tuominen, Krista
collection PubMed
description BACKGROUND: Zoonotic livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is widely spread in pig herds in many countries. However, the knowledge regarding the survival of LA-MRSA in the pig farm environment is currently limited. The aim of this study was to assess the survival of LA-MRSA on different surface materials found in the farm environment. The study investigated the survival of two different LA-MRSA strains belonging to the clonal complex (CC) 398 on four different surfaces: stainless steel, polypropylene plastic, K30 concrete and commercial concrete disk coupons. The survival of the bacteria over time was determined by the viable count method and, where possible, fitting a model to the observed data by using nonlinear least squares method to calculate the half-life ([Formula: see text] ) for different strain and material combinations. RESULTS: The study showed that the half-life of the bacteria was longer on polypropylene plastic ([Formula: see text] =11.08–15.78 days) than on stainless steel ([Formula: see text] =2.45–7.83 days). On these materials, both LA-MRSA strains survived through the 14 week observation period. The bacterial decay was fastest on the concrete surfaces, where LA-MRSA became undetectable after 3–9 weeks. CONCLUSIONS: The survival of LA-MRSA in the pig farm environment may be affected by different surface materials. A more frequent sampling protocol (< 7 days) is needed to determine the half-life on concrete surfaces.
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spelling pubmed-100318942023-03-23 Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials Tuominen, Krista Frosth, Sara Pedersen, Karl Rosendal, Thomas Sternberg Lewerin, Susanna Acta Vet Scand Research BACKGROUND: Zoonotic livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is widely spread in pig herds in many countries. However, the knowledge regarding the survival of LA-MRSA in the pig farm environment is currently limited. The aim of this study was to assess the survival of LA-MRSA on different surface materials found in the farm environment. The study investigated the survival of two different LA-MRSA strains belonging to the clonal complex (CC) 398 on four different surfaces: stainless steel, polypropylene plastic, K30 concrete and commercial concrete disk coupons. The survival of the bacteria over time was determined by the viable count method and, where possible, fitting a model to the observed data by using nonlinear least squares method to calculate the half-life ([Formula: see text] ) for different strain and material combinations. RESULTS: The study showed that the half-life of the bacteria was longer on polypropylene plastic ([Formula: see text] =11.08–15.78 days) than on stainless steel ([Formula: see text] =2.45–7.83 days). On these materials, both LA-MRSA strains survived through the 14 week observation period. The bacterial decay was fastest on the concrete surfaces, where LA-MRSA became undetectable after 3–9 weeks. CONCLUSIONS: The survival of LA-MRSA in the pig farm environment may be affected by different surface materials. A more frequent sampling protocol (< 7 days) is needed to determine the half-life on concrete surfaces. BioMed Central 2023-03-21 /pmc/articles/PMC10031894/ /pubmed/36944983 http://dx.doi.org/10.1186/s13028-023-00676-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Tuominen, Krista
Frosth, Sara
Pedersen, Karl
Rosendal, Thomas
Sternberg Lewerin, Susanna
Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials
title Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials
title_full Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials
title_fullStr Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials
title_full_unstemmed Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials
title_short Survival of livestock-associated methicillin-resistant Staphylococcus aureus CC398 on different surface materials
title_sort survival of livestock-associated methicillin-resistant staphylococcus aureus cc398 on different surface materials
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031894/
https://www.ncbi.nlm.nih.gov/pubmed/36944983
http://dx.doi.org/10.1186/s13028-023-00676-z
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