Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression

The aim of the present study was to elucidate the evolutionary trajectory of colon cells from normal colon mucosa, to adenoma, then to carcinoma in the same microenvironment. Normal colon, adenoma and carcinoma tissues from the same patient were analyzed by single-cell sequencing, which perfectly si...

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Autores principales: Sun, Ruifang, Yang, Yang, Lü, Weidong, Yang, Yanqi, Li, Yulong, Liu, Zhigang, Diao, Dongmei, Wang, Yang, Chang, Su’e, Lu, Mengnan, Jiang, Qiuyu, Dai, Bingling, Ma, Xiaobin, Zhao, Chang’an, Lü, Moqi, Zhang, Juan, Ding, Caixia, Li, Na, Zhang, Jian, Xiao, Zhengtao, Zhou, Dangxia, Huang, Chen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031920/
https://www.ncbi.nlm.nih.gov/pubmed/36944972
http://dx.doi.org/10.1186/s13578-023-01002-w
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author Sun, Ruifang
Yang, Yang
Lü, Weidong
Yang, Yanqi
Li, Yulong
Liu, Zhigang
Diao, Dongmei
Wang, Yang
Chang, Su’e
Lu, Mengnan
Jiang, Qiuyu
Dai, Bingling
Ma, Xiaobin
Zhao, Chang’an
Lü, Moqi
Zhang, Juan
Ding, Caixia
Li, Na
Zhang, Jian
Xiao, Zhengtao
Zhou, Dangxia
Huang, Chen
author_facet Sun, Ruifang
Yang, Yang
Lü, Weidong
Yang, Yanqi
Li, Yulong
Liu, Zhigang
Diao, Dongmei
Wang, Yang
Chang, Su’e
Lu, Mengnan
Jiang, Qiuyu
Dai, Bingling
Ma, Xiaobin
Zhao, Chang’an
Lü, Moqi
Zhang, Juan
Ding, Caixia
Li, Na
Zhang, Jian
Xiao, Zhengtao
Zhou, Dangxia
Huang, Chen
author_sort Sun, Ruifang
collection PubMed
description The aim of the present study was to elucidate the evolutionary trajectory of colon cells from normal colon mucosa, to adenoma, then to carcinoma in the same microenvironment. Normal colon, adenoma and carcinoma tissues from the same patient were analyzed by single-cell sequencing, which perfectly simulated the process of time-dependent colon cancer due to the same microenvironment. A total of 22 cell types were identified. Results suggest the presence of dominant clones of same cells including C2 goblet cell, epithelial cell subtype 1 (Epi1), enterocyte cell subset 0 (Entero0), and Entero5 in carcinoma. Epi1 and Entero0 were Co-enriched in antibacterial and IL-17 signaling, Entero5 was enriched in immune response and mucin-type O-glycan biosynthesis. We discovered new colon cancer related genes including AC007952.4, NEK8, CHRM3, ANO7, B3GNT6, NEURL1, ODC1 and KCNMA1. The function of TBC1D4, LTB, C2CD4A, AND GBP4/5 in T cells needs to be clarified. We used colon samples from the same person, which provide new information for colon cancer therapy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13578-023-01002-w.
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spelling pubmed-100319202023-03-23 Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression Sun, Ruifang Yang, Yang Lü, Weidong Yang, Yanqi Li, Yulong Liu, Zhigang Diao, Dongmei Wang, Yang Chang, Su’e Lu, Mengnan Jiang, Qiuyu Dai, Bingling Ma, Xiaobin Zhao, Chang’an Lü, Moqi Zhang, Juan Ding, Caixia Li, Na Zhang, Jian Xiao, Zhengtao Zhou, Dangxia Huang, Chen Cell Biosci Research The aim of the present study was to elucidate the evolutionary trajectory of colon cells from normal colon mucosa, to adenoma, then to carcinoma in the same microenvironment. Normal colon, adenoma and carcinoma tissues from the same patient were analyzed by single-cell sequencing, which perfectly simulated the process of time-dependent colon cancer due to the same microenvironment. A total of 22 cell types were identified. Results suggest the presence of dominant clones of same cells including C2 goblet cell, epithelial cell subtype 1 (Epi1), enterocyte cell subset 0 (Entero0), and Entero5 in carcinoma. Epi1 and Entero0 were Co-enriched in antibacterial and IL-17 signaling, Entero5 was enriched in immune response and mucin-type O-glycan biosynthesis. We discovered new colon cancer related genes including AC007952.4, NEK8, CHRM3, ANO7, B3GNT6, NEURL1, ODC1 and KCNMA1. The function of TBC1D4, LTB, C2CD4A, AND GBP4/5 in T cells needs to be clarified. We used colon samples from the same person, which provide new information for colon cancer therapy. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s13578-023-01002-w. BioMed Central 2023-03-21 /pmc/articles/PMC10031920/ /pubmed/36944972 http://dx.doi.org/10.1186/s13578-023-01002-w Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Sun, Ruifang
Yang, Yang
Lü, Weidong
Yang, Yanqi
Li, Yulong
Liu, Zhigang
Diao, Dongmei
Wang, Yang
Chang, Su’e
Lu, Mengnan
Jiang, Qiuyu
Dai, Bingling
Ma, Xiaobin
Zhao, Chang’an
Lü, Moqi
Zhang, Juan
Ding, Caixia
Li, Na
Zhang, Jian
Xiao, Zhengtao
Zhou, Dangxia
Huang, Chen
Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression
title Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression
title_full Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression
title_fullStr Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression
title_full_unstemmed Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression
title_short Single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression
title_sort single-cell transcriptomic analysis of normal and pathological tissues from the same patient uncovers colon cancer progression
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031920/
https://www.ncbi.nlm.nih.gov/pubmed/36944972
http://dx.doi.org/10.1186/s13578-023-01002-w
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