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Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco
BACKGROUND: Photosynthetic pigments in higher plants, including chlorophyll (Chl) and carotenoids, are crucial for photosynthesis and photoprotection. Chl-deficient tobacco seedlings generally have a lower photosynthesis rate and higher nitrate-nitrogen (NO(3)-N) content, which causes a profound inf...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031990/ https://www.ncbi.nlm.nih.gov/pubmed/36944910 http://dx.doi.org/10.1186/s12870-023-04169-z |
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author | Feng, Yuqing Li, Yujing Zhao, Yuanyuan Shi, Hongzhi |
author_facet | Feng, Yuqing Li, Yujing Zhao, Yuanyuan Shi, Hongzhi |
author_sort | Feng, Yuqing |
collection | PubMed |
description | BACKGROUND: Photosynthetic pigments in higher plants, including chlorophyll (Chl) and carotenoids, are crucial for photosynthesis and photoprotection. Chl-deficient tobacco seedlings generally have a lower photosynthesis rate and higher nitrate-nitrogen (NO(3)-N) content, which causes a profound influence on tobacco yield and quality. In this study, a stable albino leaf mutant (Al) and slight-green leaf mutant (SG) obtained from the common flue-cured tobacco (Nicotiana tabacum L.) cultivar ‘Zhongyan 100’ (ZY100) by mutagenesis with ethyl methanesulfonate (EMS) were used as materials. The differences between the Chl-deficient mutants and the wild-type (WT) were analyzed in terms of biomass, photosynthetic fluorescence parameters, and carbon- and nitrogen-related physiological parameters. RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA) were used to explore the key pathways and candidate genes regulating differentiated chlorophyll and nitrate content. RESULTS: The results showed that, when compared to the WT, the Chl content and biomass of mutant plants were considerably lower while the NO(3)-N content was substantially elevated. The net photosynthetic rate, photosynthetic fluorescence parameters, carbohydrate, soluble protein, and carbon- and nitrogen-related enzyme activities all decreased in leaves of mutants and the development of chloroplasts was abnormal. Applying more nitrogen improved the growth and development of mutants, whereas NO(3)-N content distinctively increased compared with that of the WT. Through transcriptome sequencing, the downregulated genes in mutants were enriched in plant hormone signal transduction and nitrogen metabolism, which are involved in pigment biosynthesis and the carbon fixation pathway. In addition, two hub genes and seven transcription factors identified from the blue module through WGCNA were likely to be key candidate factors involved in chlorophyll synthesis and nitrate accumulation. CONCLUSION: Our results demonstrated that differences in chlorophyll and nitrate content were caused by the combined effects of chloroplast development, photosynthesis, as well as related biological activity. In addition, transcriptome results provide a bioinformatics resource for further functional identification of key pathways and genes responsible for differences in chlorophyll and nitrate content in tobacco plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04169-z. |
format | Online Article Text |
id | pubmed-10031990 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-100319902023-03-23 Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco Feng, Yuqing Li, Yujing Zhao, Yuanyuan Shi, Hongzhi BMC Plant Biol Research BACKGROUND: Photosynthetic pigments in higher plants, including chlorophyll (Chl) and carotenoids, are crucial for photosynthesis and photoprotection. Chl-deficient tobacco seedlings generally have a lower photosynthesis rate and higher nitrate-nitrogen (NO(3)-N) content, which causes a profound influence on tobacco yield and quality. In this study, a stable albino leaf mutant (Al) and slight-green leaf mutant (SG) obtained from the common flue-cured tobacco (Nicotiana tabacum L.) cultivar ‘Zhongyan 100’ (ZY100) by mutagenesis with ethyl methanesulfonate (EMS) were used as materials. The differences between the Chl-deficient mutants and the wild-type (WT) were analyzed in terms of biomass, photosynthetic fluorescence parameters, and carbon- and nitrogen-related physiological parameters. RNA sequencing (RNA-seq) and weighted gene co-expression network analysis (WGCNA) were used to explore the key pathways and candidate genes regulating differentiated chlorophyll and nitrate content. RESULTS: The results showed that, when compared to the WT, the Chl content and biomass of mutant plants were considerably lower while the NO(3)-N content was substantially elevated. The net photosynthetic rate, photosynthetic fluorescence parameters, carbohydrate, soluble protein, and carbon- and nitrogen-related enzyme activities all decreased in leaves of mutants and the development of chloroplasts was abnormal. Applying more nitrogen improved the growth and development of mutants, whereas NO(3)-N content distinctively increased compared with that of the WT. Through transcriptome sequencing, the downregulated genes in mutants were enriched in plant hormone signal transduction and nitrogen metabolism, which are involved in pigment biosynthesis and the carbon fixation pathway. In addition, two hub genes and seven transcription factors identified from the blue module through WGCNA were likely to be key candidate factors involved in chlorophyll synthesis and nitrate accumulation. CONCLUSION: Our results demonstrated that differences in chlorophyll and nitrate content were caused by the combined effects of chloroplast development, photosynthesis, as well as related biological activity. In addition, transcriptome results provide a bioinformatics resource for further functional identification of key pathways and genes responsible for differences in chlorophyll and nitrate content in tobacco plants. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04169-z. BioMed Central 2023-03-22 /pmc/articles/PMC10031990/ /pubmed/36944910 http://dx.doi.org/10.1186/s12870-023-04169-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Feng, Yuqing Li, Yujing Zhao, Yuanyuan Shi, Hongzhi Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco |
title | Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco |
title_full | Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco |
title_fullStr | Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco |
title_full_unstemmed | Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco |
title_short | Physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco |
title_sort | physiological, transcriptome and co-expression network analysis of chlorophyll-deficient mutants in flue-cured tobacco |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10031990/ https://www.ncbi.nlm.nih.gov/pubmed/36944910 http://dx.doi.org/10.1186/s12870-023-04169-z |
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