Enhanced single RNA imaging reveals dynamic gene expression in live animals

Imaging endogenous mRNAs in live animals is technically challenging. Here, we describe an MS2-based signal amplification with the Suntag system that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1300 nt 24xMS2 into th...

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Autores principales: Hu, Yucen, Xu, Jingxiu, Gao, Erqing, Fan, Xueyuan, Wei, Jieli, Ye, Bingcheng, Xu, Suhong, Ma, Weirui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10032653/
https://www.ncbi.nlm.nih.gov/pubmed/36867026
http://dx.doi.org/10.7554/eLife.82178
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author Hu, Yucen
Xu, Jingxiu
Gao, Erqing
Fan, Xueyuan
Wei, Jieli
Ye, Bingcheng
Xu, Suhong
Ma, Weirui
author_facet Hu, Yucen
Xu, Jingxiu
Gao, Erqing
Fan, Xueyuan
Wei, Jieli
Ye, Bingcheng
Xu, Suhong
Ma, Weirui
author_sort Hu, Yucen
collection PubMed
description Imaging endogenous mRNAs in live animals is technically challenging. Here, we describe an MS2-based signal amplification with the Suntag system that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1300 nt 24xMS2 into the genome for the imaging of endogenous mRNAs. Using this tool, we were able to image the activation of gene expression and the dynamics of endogenous mRNAs in the epidermis of live C. elegans.
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spelling pubmed-100326532023-03-23 Enhanced single RNA imaging reveals dynamic gene expression in live animals Hu, Yucen Xu, Jingxiu Gao, Erqing Fan, Xueyuan Wei, Jieli Ye, Bingcheng Xu, Suhong Ma, Weirui eLife Cell Biology Imaging endogenous mRNAs in live animals is technically challenging. Here, we describe an MS2-based signal amplification with the Suntag system that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1300 nt 24xMS2 into the genome for the imaging of endogenous mRNAs. Using this tool, we were able to image the activation of gene expression and the dynamics of endogenous mRNAs in the epidermis of live C. elegans. eLife Sciences Publications, Ltd 2023-03-03 /pmc/articles/PMC10032653/ /pubmed/36867026 http://dx.doi.org/10.7554/eLife.82178 Text en © 2023, Hu, Xu, Gao et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Cell Biology
Hu, Yucen
Xu, Jingxiu
Gao, Erqing
Fan, Xueyuan
Wei, Jieli
Ye, Bingcheng
Xu, Suhong
Ma, Weirui
Enhanced single RNA imaging reveals dynamic gene expression in live animals
title Enhanced single RNA imaging reveals dynamic gene expression in live animals
title_full Enhanced single RNA imaging reveals dynamic gene expression in live animals
title_fullStr Enhanced single RNA imaging reveals dynamic gene expression in live animals
title_full_unstemmed Enhanced single RNA imaging reveals dynamic gene expression in live animals
title_short Enhanced single RNA imaging reveals dynamic gene expression in live animals
title_sort enhanced single rna imaging reveals dynamic gene expression in live animals
topic Cell Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10032653/
https://www.ncbi.nlm.nih.gov/pubmed/36867026
http://dx.doi.org/10.7554/eLife.82178
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AT weijieli enhancedsinglernaimagingrevealsdynamicgeneexpressioninliveanimals
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