Enhanced single RNA imaging reveals dynamic gene expression in live animals

Imaging endogenous mRNAs in live animals is technically challenging. Here, we describe an MS2-based signal amplification with the Suntag system that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1300 nt 24xMS2 into th...

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Detalles Bibliográficos
Autores principales: Hu, Yucen, Xu, Jingxiu, Gao, Erqing, Fan, Xueyuan, Wei, Jieli, Ye, Bingcheng, Xu, Suhong, Ma, Weirui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2023
Materias:
Cell Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10032653/
https://www.ncbi.nlm.nih.gov/pubmed/36867026
http://dx.doi.org/10.7554/eLife.82178

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10032653/
https://www.ncbi.nlm.nih.gov/pubmed/36867026
http://dx.doi.org/10.7554/eLife.82178

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