Cargando…
Enhanced single RNA imaging reveals dynamic gene expression in live animals
Imaging endogenous mRNAs in live animals is technically challenging. Here, we describe an MS2-based signal amplification with the Suntag system that enables live-cell RNA imaging of high temporal resolution and with 8xMS2 stem-loops, which overcomes the obstacle of inserting a 1300 nt 24xMS2 into th...
Autores principales: | Hu, Yucen, Xu, Jingxiu, Gao, Erqing, Fan, Xueyuan, Wei, Jieli, Ye, Bingcheng, Xu, Suhong, Ma, Weirui |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10032653/ https://www.ncbi.nlm.nih.gov/pubmed/36867026 http://dx.doi.org/10.7554/eLife.82178 |
Ejemplares similares
-
A flagellate-to-amoeboid switch in the closest living relatives of animals
por: Brunet, Thibaut, et al.
Publicado: (2021) -
In vivo reconstitution finds multivalent RNA–RNA interactions as drivers of mesh-like condensates
por: Ma, Weirui, et al.
Publicado: (2021) -
Drawing and the dynamic nature of living systems
por: Anderson, Gemma, et al.
Publicado: (2019) -
A bacterial sulfonolipid triggers multicellular development in the closest living relatives of animals
por: Alegado, Rosanna A, et al.
Publicado: (2012) -
Single molecule imaging reveals a major role for diffusion in the exploration of ciliary space by signaling receptors
por: Ye, Fan, et al.
Publicado: (2013)