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Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network

Understanding the actual distribution of different Legionella species in water networks would help prevent outbreaks. Culture investigations followed by serological agglutination tests, with poly/monovalent antisera, still represent the gold standard for isolation and identification of Legionella st...

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Autores principales: De Giglio, Osvalda, D’Ambrosio, Marilena, Spagnuolo, Valentina, Diella, Giusy, Fasano, Fabrizio, Leone, Carla Maria, Lopuzzo, Marco, Trallo, Valeria, Calia, Carla, Oliva, Marta, Pazzani, Carlo, Iacumin, Lucilla, Barigelli, Sofia, Petricciuolo, Maya, Federici, Ermanno, Lisena, Francesco Paolo, Minicucci, Anna Maria, Montagna, Maria Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer International Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033568/
https://www.ncbi.nlm.nih.gov/pubmed/36947259
http://dx.doi.org/10.1007/s10661-023-11078-z
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author De Giglio, Osvalda
D’Ambrosio, Marilena
Spagnuolo, Valentina
Diella, Giusy
Fasano, Fabrizio
Leone, Carla Maria
Lopuzzo, Marco
Trallo, Valeria
Calia, Carla
Oliva, Marta
Pazzani, Carlo
Iacumin, Lucilla
Barigelli, Sofia
Petricciuolo, Maya
Federici, Ermanno
Lisena, Francesco Paolo
Minicucci, Anna Maria
Montagna, Maria Teresa
author_facet De Giglio, Osvalda
D’Ambrosio, Marilena
Spagnuolo, Valentina
Diella, Giusy
Fasano, Fabrizio
Leone, Carla Maria
Lopuzzo, Marco
Trallo, Valeria
Calia, Carla
Oliva, Marta
Pazzani, Carlo
Iacumin, Lucilla
Barigelli, Sofia
Petricciuolo, Maya
Federici, Ermanno
Lisena, Francesco Paolo
Minicucci, Anna Maria
Montagna, Maria Teresa
author_sort De Giglio, Osvalda
collection PubMed
description Understanding the actual distribution of different Legionella species in water networks would help prevent outbreaks. Culture investigations followed by serological agglutination tests, with poly/monovalent antisera, still represent the gold standard for isolation and identification of Legionella strains. However, also MALDI-TOF and mip-gene sequencing are currently used. This study was conducted to genetically correlate strains of Legionella non pneumophila (L-np) isolated during environmental surveillance comparing different molecular techniques. Overall, 346 water samples were collected from the water system of four pavilions located in a hospital of the Apulia Region of Italy. Strains isolated from the samples were then identified by serological tests, MALDI-TOF, and mip-gene sequencing. Overall, 24.9% of water samples were positive for Legionella, among which the majority were Legionella pneumophila (Lpn) 1 (52.3%), followed by Lpn2-15 (20.9%), L-np (17.4%), Lpn1 + Lpn2-15 (7.1%), and L-np + Lpn1 (2.3%). Initially, L-np strains were identified as L. bozemanii by monovalent antiserum, while MALDI-TOF and mip-gene sequencing assigned them to L. anisa. More cold water than hot water samples were contaminated by L. anisa (p < 0.001). PFGE, RAPD, Rep-PCR, and SAU-PCR were performed to correlate L. anisa strains. Eleven out of 14 strains identified in all four pavilions showed 100% of similarity upon PFGE analysis. RAPD, Rep-PCR, and SAU-PCR showed greater discriminative power than PFGE.
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spelling pubmed-100335682023-03-24 Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network De Giglio, Osvalda D’Ambrosio, Marilena Spagnuolo, Valentina Diella, Giusy Fasano, Fabrizio Leone, Carla Maria Lopuzzo, Marco Trallo, Valeria Calia, Carla Oliva, Marta Pazzani, Carlo Iacumin, Lucilla Barigelli, Sofia Petricciuolo, Maya Federici, Ermanno Lisena, Francesco Paolo Minicucci, Anna Maria Montagna, Maria Teresa Environ Monit Assess Article Understanding the actual distribution of different Legionella species in water networks would help prevent outbreaks. Culture investigations followed by serological agglutination tests, with poly/monovalent antisera, still represent the gold standard for isolation and identification of Legionella strains. However, also MALDI-TOF and mip-gene sequencing are currently used. This study was conducted to genetically correlate strains of Legionella non pneumophila (L-np) isolated during environmental surveillance comparing different molecular techniques. Overall, 346 water samples were collected from the water system of four pavilions located in a hospital of the Apulia Region of Italy. Strains isolated from the samples were then identified by serological tests, MALDI-TOF, and mip-gene sequencing. Overall, 24.9% of water samples were positive for Legionella, among which the majority were Legionella pneumophila (Lpn) 1 (52.3%), followed by Lpn2-15 (20.9%), L-np (17.4%), Lpn1 + Lpn2-15 (7.1%), and L-np + Lpn1 (2.3%). Initially, L-np strains were identified as L. bozemanii by monovalent antiserum, while MALDI-TOF and mip-gene sequencing assigned them to L. anisa. More cold water than hot water samples were contaminated by L. anisa (p < 0.001). PFGE, RAPD, Rep-PCR, and SAU-PCR were performed to correlate L. anisa strains. Eleven out of 14 strains identified in all four pavilions showed 100% of similarity upon PFGE analysis. RAPD, Rep-PCR, and SAU-PCR showed greater discriminative power than PFGE. Springer International Publishing 2023-03-22 2023 /pmc/articles/PMC10033568/ /pubmed/36947259 http://dx.doi.org/10.1007/s10661-023-11078-z Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
De Giglio, Osvalda
D’Ambrosio, Marilena
Spagnuolo, Valentina
Diella, Giusy
Fasano, Fabrizio
Leone, Carla Maria
Lopuzzo, Marco
Trallo, Valeria
Calia, Carla
Oliva, Marta
Pazzani, Carlo
Iacumin, Lucilla
Barigelli, Sofia
Petricciuolo, Maya
Federici, Ermanno
Lisena, Francesco Paolo
Minicucci, Anna Maria
Montagna, Maria Teresa
Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network
title Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network
title_full Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network
title_fullStr Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network
title_full_unstemmed Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network
title_short Legionella anisa or Legionella bozemanii? Traditional and molecular techniques as support in the environmental surveillance of a hospital water network
title_sort legionella anisa or legionella bozemanii? traditional and molecular techniques as support in the environmental surveillance of a hospital water network
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033568/
https://www.ncbi.nlm.nih.gov/pubmed/36947259
http://dx.doi.org/10.1007/s10661-023-11078-z
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