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Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome
It is necessary to assess the appropriate dietary protein level of the forest musk deer (FMD), as nutritional needs are unclear. The microbiome in gastrointestinal tracts plays an important role in regulating nutrient utilization, absorption and host growth or development. Thus, we aimed to evaluate...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033606/ https://www.ncbi.nlm.nih.gov/pubmed/36970665 http://dx.doi.org/10.3389/fmicb.2023.1124163 |
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author | Gong, Ruiguang Song, Shengjie Ai, Yaotian Wang, Shuhui Dong, Xianggui Ren, Zhanjun Xie, Hui Jiang, Benmo Zhao, Lixia |
author_facet | Gong, Ruiguang Song, Shengjie Ai, Yaotian Wang, Shuhui Dong, Xianggui Ren, Zhanjun Xie, Hui Jiang, Benmo Zhao, Lixia |
author_sort | Gong, Ruiguang |
collection | PubMed |
description | It is necessary to assess the appropriate dietary protein level of the forest musk deer (FMD), as nutritional needs are unclear. The microbiome in gastrointestinal tracts plays an important role in regulating nutrient utilization, absorption and host growth or development. Thus, we aimed to evaluate growth performance, nutrient digestibility and fecal microbiome of growing FMD supplied with different protein levels of diets. Eighteen 6-month-old male FMD with an initial weight 5.0 ± 0.2 kg were used in a 62-day trial. The animals were randomly distributed to three groups, the dietary crude protein (CP) level was 11.51% (L), 13.37% (M), and 15.48% (H). The results showed that the CP digestibility decreased as dietary CP level increased (p < 0.01). Compared with group L and H, FMD in M group has higher average daily gain, feed efficiency and neutral detergent fiber digestibility. For the fecal bacterial community, the percentage of Firmicutes was increased, Bacteroidetes was decreased and the diversity of microbiota significantly reduced (p < 0.05) with the increasing of dietary protein. The proportion of Ruminococcaceae_005, Ruminococcaceae_UCG-014 and uncultured_bacterium_f_Lachnospiraceae were significantly increased wtih rising CP, the proportions of Bacteroides and Rikenellaceae_RC9_gut_group were significantly decrease nevertheless at the genus level. The higher abundance of f_Prevotellaceae and g_Prevotellaceae_UCG_004 were found at M group by LEfSe analysis. The relative abundance of uncultured_bacterium_f_Ruminococcaceae was positively correlated with the average daily gain and feed conversion ratio (p < 0.05), whereas Family_XIII_AD3011_group was negatively correlated with feed conversion ratio (p < 0.05). The UPGMA tree showed L and M groups were closer in clustering relationship, while H group was clustered separately into a branch, which indicated that the bacterial structure had changed greatly with protein level increased from 13.37 to 15.48%. Overall, our results indicated that the optimum dietary CP for the growing FMD was 13.37%. |
format | Online Article Text |
id | pubmed-10033606 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100336062023-03-24 Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome Gong, Ruiguang Song, Shengjie Ai, Yaotian Wang, Shuhui Dong, Xianggui Ren, Zhanjun Xie, Hui Jiang, Benmo Zhao, Lixia Front Microbiol Microbiology It is necessary to assess the appropriate dietary protein level of the forest musk deer (FMD), as nutritional needs are unclear. The microbiome in gastrointestinal tracts plays an important role in regulating nutrient utilization, absorption and host growth or development. Thus, we aimed to evaluate growth performance, nutrient digestibility and fecal microbiome of growing FMD supplied with different protein levels of diets. Eighteen 6-month-old male FMD with an initial weight 5.0 ± 0.2 kg were used in a 62-day trial. The animals were randomly distributed to three groups, the dietary crude protein (CP) level was 11.51% (L), 13.37% (M), and 15.48% (H). The results showed that the CP digestibility decreased as dietary CP level increased (p < 0.01). Compared with group L and H, FMD in M group has higher average daily gain, feed efficiency and neutral detergent fiber digestibility. For the fecal bacterial community, the percentage of Firmicutes was increased, Bacteroidetes was decreased and the diversity of microbiota significantly reduced (p < 0.05) with the increasing of dietary protein. The proportion of Ruminococcaceae_005, Ruminococcaceae_UCG-014 and uncultured_bacterium_f_Lachnospiraceae were significantly increased wtih rising CP, the proportions of Bacteroides and Rikenellaceae_RC9_gut_group were significantly decrease nevertheless at the genus level. The higher abundance of f_Prevotellaceae and g_Prevotellaceae_UCG_004 were found at M group by LEfSe analysis. The relative abundance of uncultured_bacterium_f_Ruminococcaceae was positively correlated with the average daily gain and feed conversion ratio (p < 0.05), whereas Family_XIII_AD3011_group was negatively correlated with feed conversion ratio (p < 0.05). The UPGMA tree showed L and M groups were closer in clustering relationship, while H group was clustered separately into a branch, which indicated that the bacterial structure had changed greatly with protein level increased from 13.37 to 15.48%. Overall, our results indicated that the optimum dietary CP for the growing FMD was 13.37%. Frontiers Media S.A. 2023-03-09 /pmc/articles/PMC10033606/ /pubmed/36970665 http://dx.doi.org/10.3389/fmicb.2023.1124163 Text en Copyright © 2023 Gong, Song, Ai, Wang, Dong, Ren, Xie, Jiang and Zhao. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Gong, Ruiguang Song, Shengjie Ai, Yaotian Wang, Shuhui Dong, Xianggui Ren, Zhanjun Xie, Hui Jiang, Benmo Zhao, Lixia Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome |
title | Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome |
title_full | Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome |
title_fullStr | Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome |
title_full_unstemmed | Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome |
title_short | Exploring the growing forest musk deer (Moschus berezovskii) dietary protein requirement based on gut microbiome |
title_sort | exploring the growing forest musk deer (moschus berezovskii) dietary protein requirement based on gut microbiome |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033606/ https://www.ncbi.nlm.nih.gov/pubmed/36970665 http://dx.doi.org/10.3389/fmicb.2023.1124163 |
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