Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry

The role of innate lymphoid cells (ILCs)—including natural killer cells, helper-like ILC1s, ILC2s, ILC3s, and lymphoid tissue inducers—in human cancer is still poorly understood due to the scarcity of cell number. To address this, we present a protocol to analyze or purify ILCs from human blood, adj...

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Autores principales: Qi, Jingjing, Hong, Liwen, Wang, Zhengting, Liu, Yingbin, Shen, Lei, Su, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033740/
https://www.ncbi.nlm.nih.gov/pubmed/36943862
http://dx.doi.org/10.1016/j.xpro.2023.102180
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author Qi, Jingjing
Hong, Liwen
Wang, Zhengting
Liu, Yingbin
Shen, Lei
Su, Bing
author_facet Qi, Jingjing
Hong, Liwen
Wang, Zhengting
Liu, Yingbin
Shen, Lei
Su, Bing
author_sort Qi, Jingjing
collection PubMed
description The role of innate lymphoid cells (ILCs)—including natural killer cells, helper-like ILC1s, ILC2s, ILC3s, and lymphoid tissue inducers—in human cancer is still poorly understood due to the scarcity of cell number. To address this, we present a protocol to analyze or purify ILCs from human blood, adjacent intestine, and colorectal tumor tissue. We describe steps for tissue and blood treatment, density centrifugation, antibody staining, and cell sorting. For complete details on the use and execution of this protocol, please refer to Qi et al. (2021).(1)
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spelling pubmed-100337402023-03-24 Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry Qi, Jingjing Hong, Liwen Wang, Zhengting Liu, Yingbin Shen, Lei Su, Bing STAR Protoc Protocol The role of innate lymphoid cells (ILCs)—including natural killer cells, helper-like ILC1s, ILC2s, ILC3s, and lymphoid tissue inducers—in human cancer is still poorly understood due to the scarcity of cell number. To address this, we present a protocol to analyze or purify ILCs from human blood, adjacent intestine, and colorectal tumor tissue. We describe steps for tissue and blood treatment, density centrifugation, antibody staining, and cell sorting. For complete details on the use and execution of this protocol, please refer to Qi et al. (2021).(1) Elsevier 2023-03-20 /pmc/articles/PMC10033740/ /pubmed/36943862 http://dx.doi.org/10.1016/j.xpro.2023.102180 Text en © 2023. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Qi, Jingjing
Hong, Liwen
Wang, Zhengting
Liu, Yingbin
Shen, Lei
Su, Bing
Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry
title Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry
title_full Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry
title_fullStr Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry
title_full_unstemmed Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry
title_short Analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry
title_sort analysis and purification of innate lymphoid cells in human intestine and blood by flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10033740/
https://www.ncbi.nlm.nih.gov/pubmed/36943862
http://dx.doi.org/10.1016/j.xpro.2023.102180
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