Cargando…

Lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in Candida-administered bilateral nephrectomy mice

The impacts of metabolomic changes (reduced short-chain-fatty acids; SCFAs) in uremic condition is not fully understood. Once daily Candida gavage with or without probiotics (different times of administration) for 1 week prior to bilateral nephrectomy (Bil Nep) in 8-week-old C57BL6 mice as the possi...

Descripción completa

Detalles Bibliográficos
Autores principales: Chancharoenthana, Wiwat, Kamolratanakul, Supitcha, Visitchanakun, Peerapat, Sontidejkul, Supistha, Cheibchalard, Thanya, Somboonna, Naraporn, Settachaimongkon, Sarn, Leelahavanichkul, Asada
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034098/
https://www.ncbi.nlm.nih.gov/pubmed/36969207
http://dx.doi.org/10.3389/fimmu.2023.1131447
_version_ 1784911135797936128
author Chancharoenthana, Wiwat
Kamolratanakul, Supitcha
Visitchanakun, Peerapat
Sontidejkul, Supistha
Cheibchalard, Thanya
Somboonna, Naraporn
Settachaimongkon, Sarn
Leelahavanichkul, Asada
author_facet Chancharoenthana, Wiwat
Kamolratanakul, Supitcha
Visitchanakun, Peerapat
Sontidejkul, Supistha
Cheibchalard, Thanya
Somboonna, Naraporn
Settachaimongkon, Sarn
Leelahavanichkul, Asada
author_sort Chancharoenthana, Wiwat
collection PubMed
description The impacts of metabolomic changes (reduced short-chain-fatty acids; SCFAs) in uremic condition is not fully understood. Once daily Candida gavage with or without probiotics (different times of administration) for 1 week prior to bilateral nephrectomy (Bil Nep) in 8-week-old C57BL6 mice as the possible models more resemble human conditions were performed. Candida-administered Bil Nep mice demonstrated more severe conditions than Bil Nep alone as indicated by mortality (n = 10/group) and other 48 h parameters (n = 6-8/group), including serum cytokines, leaky gut (FITC-dextran assay, endotoxemia, serum beta-glucan, and loss of Zona-occludens-1), and dysbiosis (increased Enterobacteriaceae with decreased diversity in microbiome analysis) (n = 3/group for fecal microbiome) without the difference in uremia (serum creatinine). With nuclear magnetic resonance metabolome analysis (n = 3-5/group), Bil Nep reduced fecal butyric (and propionic) acid and blood 3-hydroxy butyrate compared with sham and Candida-Bil Nep altered metabolomic patterns compared with Bil Nep alone. Then, Lacticaseibacillus rhamnosus dfa1 (SCFA-producing Lacticaseibacilli) (n = 8/group) attenuated the model severity (mortality, leaky gut, serum cytokines, and increased fecal butyrate) of Bil Nep mice (n = 6/group) (regardless of Candida). In enterocytes (Caco-2 cells), butyrate attenuated injury induced by indoxyl sulfate (a gut-derived uremic toxin) as indicated by transepithelial electrical resistance, supernatant IL-8, NFκB expression, and cell energy status (mitochondria and glycolysis activities by extracellular flux analysis). In conclusion, the reduced butyrate by uremia was not enhanced by Candida administration; however, the presence of Candida in the gut induced a leaky gut that was attenuated by SCFA-producing probiotics. Our data support the use of probiotics in uremia.
format Online
Article
Text
id pubmed-10034098
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-100340982023-03-24 Lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in Candida-administered bilateral nephrectomy mice Chancharoenthana, Wiwat Kamolratanakul, Supitcha Visitchanakun, Peerapat Sontidejkul, Supistha Cheibchalard, Thanya Somboonna, Naraporn Settachaimongkon, Sarn Leelahavanichkul, Asada Front Immunol Immunology The impacts of metabolomic changes (reduced short-chain-fatty acids; SCFAs) in uremic condition is not fully understood. Once daily Candida gavage with or without probiotics (different times of administration) for 1 week prior to bilateral nephrectomy (Bil Nep) in 8-week-old C57BL6 mice as the possible models more resemble human conditions were performed. Candida-administered Bil Nep mice demonstrated more severe conditions than Bil Nep alone as indicated by mortality (n = 10/group) and other 48 h parameters (n = 6-8/group), including serum cytokines, leaky gut (FITC-dextran assay, endotoxemia, serum beta-glucan, and loss of Zona-occludens-1), and dysbiosis (increased Enterobacteriaceae with decreased diversity in microbiome analysis) (n = 3/group for fecal microbiome) without the difference in uremia (serum creatinine). With nuclear magnetic resonance metabolome analysis (n = 3-5/group), Bil Nep reduced fecal butyric (and propionic) acid and blood 3-hydroxy butyrate compared with sham and Candida-Bil Nep altered metabolomic patterns compared with Bil Nep alone. Then, Lacticaseibacillus rhamnosus dfa1 (SCFA-producing Lacticaseibacilli) (n = 8/group) attenuated the model severity (mortality, leaky gut, serum cytokines, and increased fecal butyrate) of Bil Nep mice (n = 6/group) (regardless of Candida). In enterocytes (Caco-2 cells), butyrate attenuated injury induced by indoxyl sulfate (a gut-derived uremic toxin) as indicated by transepithelial electrical resistance, supernatant IL-8, NFκB expression, and cell energy status (mitochondria and glycolysis activities by extracellular flux analysis). In conclusion, the reduced butyrate by uremia was not enhanced by Candida administration; however, the presence of Candida in the gut induced a leaky gut that was attenuated by SCFA-producing probiotics. Our data support the use of probiotics in uremia. Frontiers Media S.A. 2023-03-09 /pmc/articles/PMC10034098/ /pubmed/36969207 http://dx.doi.org/10.3389/fimmu.2023.1131447 Text en Copyright © 2023 Chancharoenthana, Kamolratanakul, Visitchanakun, Sontidejkul, Cheibchalard, Somboonna, Settachaimongkon and Leelahavanichkul https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Chancharoenthana, Wiwat
Kamolratanakul, Supitcha
Visitchanakun, Peerapat
Sontidejkul, Supistha
Cheibchalard, Thanya
Somboonna, Naraporn
Settachaimongkon, Sarn
Leelahavanichkul, Asada
Lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in Candida-administered bilateral nephrectomy mice
title Lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in Candida-administered bilateral nephrectomy mice
title_full Lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in Candida-administered bilateral nephrectomy mice
title_fullStr Lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in Candida-administered bilateral nephrectomy mice
title_full_unstemmed Lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in Candida-administered bilateral nephrectomy mice
title_short Lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in Candida-administered bilateral nephrectomy mice
title_sort lacticaseibacilli attenuated fecal dysbiosis and metabolome changes in candida-administered bilateral nephrectomy mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034098/
https://www.ncbi.nlm.nih.gov/pubmed/36969207
http://dx.doi.org/10.3389/fimmu.2023.1131447
work_keys_str_mv AT chancharoenthanawiwat lacticaseibacilliattenuatedfecaldysbiosisandmetabolomechangesincandidaadministeredbilateralnephrectomymice
AT kamolratanakulsupitcha lacticaseibacilliattenuatedfecaldysbiosisandmetabolomechangesincandidaadministeredbilateralnephrectomymice
AT visitchanakunpeerapat lacticaseibacilliattenuatedfecaldysbiosisandmetabolomechangesincandidaadministeredbilateralnephrectomymice
AT sontidejkulsupistha lacticaseibacilliattenuatedfecaldysbiosisandmetabolomechangesincandidaadministeredbilateralnephrectomymice
AT cheibchalardthanya lacticaseibacilliattenuatedfecaldysbiosisandmetabolomechangesincandidaadministeredbilateralnephrectomymice
AT somboonnanaraporn lacticaseibacilliattenuatedfecaldysbiosisandmetabolomechangesincandidaadministeredbilateralnephrectomymice
AT settachaimongkonsarn lacticaseibacilliattenuatedfecaldysbiosisandmetabolomechangesincandidaadministeredbilateralnephrectomymice
AT leelahavanichkulasada lacticaseibacilliattenuatedfecaldysbiosisandmetabolomechangesincandidaadministeredbilateralnephrectomymice