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Variation in the chemical profiles of three foxglove species in the central Balkans

The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species (D. lanata, D. ferruginea, and D. grandiflora) from the central Balkans. Despite the steady use of foxglov...

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Autores principales: Gašić, Uroš, Banjanac, Tijana, Šiler, Branislav, Božunović, Jelena, Milutinović, Milica, Aničić, Neda, Dmitrović, Slavica, Skorić, Marijana, Nestorović Živković, Jasmina, Petrović, Luka, Todorović, Miloš, Živković, Suzana, Matekalo, Dragana, Filipović, Biljana, Lukić, Tamara, Mišić, Danijela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034326/
https://www.ncbi.nlm.nih.gov/pubmed/36968406
http://dx.doi.org/10.3389/fpls.2023.1155297
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author Gašić, Uroš
Banjanac, Tijana
Šiler, Branislav
Božunović, Jelena
Milutinović, Milica
Aničić, Neda
Dmitrović, Slavica
Skorić, Marijana
Nestorović Živković, Jasmina
Petrović, Luka
Todorović, Miloš
Živković, Suzana
Matekalo, Dragana
Filipović, Biljana
Lukić, Tamara
Mišić, Danijela
author_facet Gašić, Uroš
Banjanac, Tijana
Šiler, Branislav
Božunović, Jelena
Milutinović, Milica
Aničić, Neda
Dmitrović, Slavica
Skorić, Marijana
Nestorović Živković, Jasmina
Petrović, Luka
Todorović, Miloš
Živković, Suzana
Matekalo, Dragana
Filipović, Biljana
Lukić, Tamara
Mišić, Danijela
author_sort Gašić, Uroš
collection PubMed
description The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species (D. lanata, D. ferruginea, and D. grandiflora) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis (Plantaginaceae) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora. The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p-coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p-coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea. However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis.
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spelling pubmed-100343262023-03-24 Variation in the chemical profiles of three foxglove species in the central Balkans Gašić, Uroš Banjanac, Tijana Šiler, Branislav Božunović, Jelena Milutinović, Milica Aničić, Neda Dmitrović, Slavica Skorić, Marijana Nestorović Živković, Jasmina Petrović, Luka Todorović, Miloš Živković, Suzana Matekalo, Dragana Filipović, Biljana Lukić, Tamara Mišić, Danijela Front Plant Sci Plant Science The aim of this study was to determine intra- and interspecies variation in the qualitative and quantitative composition of methanol-soluble metabolites in the leaves of three Digitalis species (D. lanata, D. ferruginea, and D. grandiflora) from the central Balkans. Despite the steady use of foxglove constituents for human health as valuable medicinal products, populations of the genus Digitalis (Plantaginaceae) have been poorly investigated to describe their genetic and phenetic variation. Following untargeted profiling using UHPLC-LTQ Orbitrap MS, by which we identified a total of 115 compounds, 16 compounds were quantified using the UHPLC(–)HESI–QqQ-MS/MS approach. In total, 55 steroid compounds, 15 phenylethanoid glycosides, 27 flavonoids, and 14 phenolic acid derivatives were identified across the samples with D. lanata and D. ferruginea showing a great similarity, while 15 compounds were characteristic only for D. grandiflora. The phytochemical composition of methanol extracts, considered here as complex phenotypes, are further examined along multiple levels of biological organization (intra- and interpopulation) and subsequently subjected to chemometric data analysis. The quantitative composition of the selected set of 16 chemomarkers belonging to the classes of cardenolides (3 compounds) and phenolics (13 compounds) pointed to considerable differences between the taxa studied. D. grandiflora and D. ferruginea were found to be richer in phenolics as compared to cardenolides, which otherwise predominate in D. lanata over other compounds. PCA revealed lanatoside C, deslanoside, hispidulin, and p-coumaric acid to be the main compounds contributing to the differences between D. lanata on one side and D. grandiflora and D. ferruginea on the other, while p-coumaric acid, hispidulin, and digoxin contribute to the diversification between D. grandiflora and D. ferruginea. However, quantitative variation in the metabolite content within species was faint with mild population diversification visible in D. grandiflora and particularly in D. ferruginea. This pointed to the highly conserved content and ratio of targeted compounds within the analyzed species, which was not severely influenced by the geographic origin or environmental conditions. The presented metabolomics approach might have, along with morphometrics and molecular genetics studies, a high information value for further elucidation of the relationships among taxa within the genus Digitalis. Frontiers Media S.A. 2023-03-09 /pmc/articles/PMC10034326/ /pubmed/36968406 http://dx.doi.org/10.3389/fpls.2023.1155297 Text en Copyright © 2023 Gašić, Banjanac, Šiler, Božunović, Milutinović, Aničić, Dmitrović, Skorić, Nestorović Živković, Petrović, Todorović, Živković, Matekalo, Filipović, Lukić and Mišić https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Gašić, Uroš
Banjanac, Tijana
Šiler, Branislav
Božunović, Jelena
Milutinović, Milica
Aničić, Neda
Dmitrović, Slavica
Skorić, Marijana
Nestorović Živković, Jasmina
Petrović, Luka
Todorović, Miloš
Živković, Suzana
Matekalo, Dragana
Filipović, Biljana
Lukić, Tamara
Mišić, Danijela
Variation in the chemical profiles of three foxglove species in the central Balkans
title Variation in the chemical profiles of three foxglove species in the central Balkans
title_full Variation in the chemical profiles of three foxglove species in the central Balkans
title_fullStr Variation in the chemical profiles of three foxglove species in the central Balkans
title_full_unstemmed Variation in the chemical profiles of three foxglove species in the central Balkans
title_short Variation in the chemical profiles of three foxglove species in the central Balkans
title_sort variation in the chemical profiles of three foxglove species in the central balkans
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034326/
https://www.ncbi.nlm.nih.gov/pubmed/36968406
http://dx.doi.org/10.3389/fpls.2023.1155297
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