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Analyzing protein intermediate interactions in living E. coli cells using site-specific photo-crosslinking combined with chemical crosslinking

Information on protein-protein interactions is crucial in understanding protein-mediated cellular processes; however, analyzing transient and unstable interactions in living cells is challenging. Here, we present a protocol capturing the interaction between an assembly intermediate form of a bacteri...

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Detalles Bibliográficos
Autores principales: Miyazaki, Ryoji, Akiyama, Yoshinori
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034496/
https://www.ncbi.nlm.nih.gov/pubmed/36933223
http://dx.doi.org/10.1016/j.xpro.2023.102178