Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination

Developing an in vitro platform to study neuron-oligodendrocyte interaction, particularly myelination, is essential to understand aberrant myelination in neuropsychiatric and neurodegenerative diseases. Here, we provide a controlled, direct co-culture protocol for human induced-pluripotent-stem-cell...

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Autores principales: von der Bey, Martina, De Cicco, Silvia, Zach, Susanne, Hengerer, Bastian, Ercan-Herbst, Ebru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034497/
https://www.ncbi.nlm.nih.gov/pubmed/36933222
http://dx.doi.org/10.1016/j.xpro.2023.102164
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author von der Bey, Martina
De Cicco, Silvia
Zach, Susanne
Hengerer, Bastian
Ercan-Herbst, Ebru
author_facet von der Bey, Martina
De Cicco, Silvia
Zach, Susanne
Hengerer, Bastian
Ercan-Herbst, Ebru
author_sort von der Bey, Martina
collection PubMed
description Developing an in vitro platform to study neuron-oligodendrocyte interaction, particularly myelination, is essential to understand aberrant myelination in neuropsychiatric and neurodegenerative diseases. Here, we provide a controlled, direct co-culture protocol for human induced-pluripotent-stem-cell (hiPSC)-derived neurons and oligodendrocytes on three-dimensional (3D) nanomatrix plates. We describe steps to differentiate hiPSCs into cortical neurons and oligodendrocyte lineage cells on 3D nanofibers. We then detail the detachment and isolation of the oligodendrocyte lineage cells, followed by neuron-oligodendrocyte co-culture in this 3D microenvironment.
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spelling pubmed-100344972023-03-24 Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination von der Bey, Martina De Cicco, Silvia Zach, Susanne Hengerer, Bastian Ercan-Herbst, Ebru STAR Protoc Protocol Developing an in vitro platform to study neuron-oligodendrocyte interaction, particularly myelination, is essential to understand aberrant myelination in neuropsychiatric and neurodegenerative diseases. Here, we provide a controlled, direct co-culture protocol for human induced-pluripotent-stem-cell (hiPSC)-derived neurons and oligodendrocytes on three-dimensional (3D) nanomatrix plates. We describe steps to differentiate hiPSCs into cortical neurons and oligodendrocyte lineage cells on 3D nanofibers. We then detail the detachment and isolation of the oligodendrocyte lineage cells, followed by neuron-oligodendrocyte co-culture in this 3D microenvironment. Elsevier 2023-03-17 /pmc/articles/PMC10034497/ /pubmed/36933222 http://dx.doi.org/10.1016/j.xpro.2023.102164 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
von der Bey, Martina
De Cicco, Silvia
Zach, Susanne
Hengerer, Bastian
Ercan-Herbst, Ebru
Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination
title Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination
title_full Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination
title_fullStr Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination
title_full_unstemmed Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination
title_short Three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination
title_sort three-dimensional co-culture platform of human induced pluripotent stem cell-derived oligodendrocyte lineage cells and neurons for studying myelination
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034497/
https://www.ncbi.nlm.nih.gov/pubmed/36933222
http://dx.doi.org/10.1016/j.xpro.2023.102164
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