Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model

Epidermal cells integrate multiple signals that activate the signaling pathways involved in skin homeostasis. TGF-β1 signaling pathway upregulates microRNA (miR)-21-5p in keratinocytes and is often deregulated in skin diseases. To identify the bioactive compounds that enable to modulate the TGF-β1/m...

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Autores principales: Henriet, Elodie, Abdallah, Florence, Laurent, Yoan, Guimpied, Cyril, Clement, Emily, Simon, Michel, Pichon, Chantal, Baril, Patrick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034514/
https://www.ncbi.nlm.nih.gov/pubmed/36968096
http://dx.doi.org/10.1016/j.xjidi.2022.100175
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author Henriet, Elodie
Abdallah, Florence
Laurent, Yoan
Guimpied, Cyril
Clement, Emily
Simon, Michel
Pichon, Chantal
Baril, Patrick
author_facet Henriet, Elodie
Abdallah, Florence
Laurent, Yoan
Guimpied, Cyril
Clement, Emily
Simon, Michel
Pichon, Chantal
Baril, Patrick
author_sort Henriet, Elodie
collection PubMed
description Epidermal cells integrate multiple signals that activate the signaling pathways involved in skin homeostasis. TGF-β1 signaling pathway upregulates microRNA (miR)-21-5p in keratinocytes and is often deregulated in skin diseases. To identify the bioactive compounds that enable to modulate the TGF-β1/miR-21-5p signaling pathway, we screened a library of medicinal plant extracts using our miR-ON RILES luciferase reporter system placed under the control of the miR-21-5p in keratinocytes treated with TGF-β1. We identified silymarin, a mixture of flavonolignans extracted from Silybum marianum
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spelling pubmed-100345142023-03-24 Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model Henriet, Elodie Abdallah, Florence Laurent, Yoan Guimpied, Cyril Clement, Emily Simon, Michel Pichon, Chantal Baril, Patrick JID Innov Original Article Epidermal cells integrate multiple signals that activate the signaling pathways involved in skin homeostasis. TGF-β1 signaling pathway upregulates microRNA (miR)-21-5p in keratinocytes and is often deregulated in skin diseases. To identify the bioactive compounds that enable to modulate the TGF-β1/miR-21-5p signaling pathway, we screened a library of medicinal plant extracts using our miR-ON RILES luciferase reporter system placed under the control of the miR-21-5p in keratinocytes treated with TGF-β1. We identified silymarin, a mixture of flavonolignans extracted from Silybum marianum Elsevier 2022-12-16 /pmc/articles/PMC10034514/ /pubmed/36968096 http://dx.doi.org/10.1016/j.xjidi.2022.100175 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Henriet, Elodie
Abdallah, Florence
Laurent, Yoan
Guimpied, Cyril
Clement, Emily
Simon, Michel
Pichon, Chantal
Baril, Patrick
Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model
title Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model
title_full Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model
title_fullStr Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model
title_full_unstemmed Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model
title_short Targeting TGF-β1/miR-21 Pathway in Keratinocytes Reveals Protective Effects of Silymarin on Imiquimod-Induced Psoriasis Mouse Model
title_sort targeting tgf-β1/mir-21 pathway in keratinocytes reveals protective effects of silymarin on imiquimod-induced psoriasis mouse model
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034514/
https://www.ncbi.nlm.nih.gov/pubmed/36968096
http://dx.doi.org/10.1016/j.xjidi.2022.100175
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