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Identification of Nocardia and non‐tuberculous Mycobacterium species by MALDI‐TOF MS using the VITEK MS coupled to IVD and RUO databases

Identification of Nocardia and Mycobacterium species by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry (MALDI‐TOF MS) is still a challenging task that requires both suitable protein extraction procedures and extensive databases. This study aimed to evaluate the VITEK MS...

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Autores principales: Rodríguez‐Temporal, David, Zvezdánova, Margarita Estreya, Benedí, Pablo, Marín, Mercedes, Blázquez‐Sánchez, Mario, Ruiz‐Serrano, María Jesús, Muñoz, Patricia, Rodríguez‐Sánchez, Belén
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034632/
https://www.ncbi.nlm.nih.gov/pubmed/36541026
http://dx.doi.org/10.1111/1751-7915.14146
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author Rodríguez‐Temporal, David
Zvezdánova, Margarita Estreya
Benedí, Pablo
Marín, Mercedes
Blázquez‐Sánchez, Mario
Ruiz‐Serrano, María Jesús
Muñoz, Patricia
Rodríguez‐Sánchez, Belén
author_facet Rodríguez‐Temporal, David
Zvezdánova, Margarita Estreya
Benedí, Pablo
Marín, Mercedes
Blázquez‐Sánchez, Mario
Ruiz‐Serrano, María Jesús
Muñoz, Patricia
Rodríguez‐Sánchez, Belén
author_sort Rodríguez‐Temporal, David
collection PubMed
description Identification of Nocardia and Mycobacterium species by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry (MALDI‐TOF MS) is still a challenging task that requires both suitable protein extraction procedures and extensive databases. This study aimed to evaluate the VITEK MS Plus system coupled with updated RUO (v4.17) and IVD (v3.2) databases for the identification of Nocardia spp. and Mycobacterium spp. clinical isolates. Sample preparation was carried out using the VITEK MS Mycobacterium/Nocardia kit for protein extraction. From 90 Nocardia spp. isolates analysed, 86 (95.6%) were correctly identified at species or complex level using IVD and 78 (86.7%) using RUO. Only two strains were misidentified as other species pertaining to the same complex. Among the 106 non‐tuberculous Mycobacterium clinical isolates tested from a liquid culture medium, VITEK MS identified correctly at species or complex level 96 (90.6%) isolates in the IVD mode and 89 (84.0%) isolates in the RUO mode. No misidentifications were detected. Although the IVD mode was unable to differentiate members of the M. fortuitum complex, the RUO mode correctly discriminated M. peregrinum and M. septicum. The robustness and accuracy showed by this system allow its implementation for routine identification of these microorganisms in clinical laboratories.
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spelling pubmed-100346322023-03-24 Identification of Nocardia and non‐tuberculous Mycobacterium species by MALDI‐TOF MS using the VITEK MS coupled to IVD and RUO databases Rodríguez‐Temporal, David Zvezdánova, Margarita Estreya Benedí, Pablo Marín, Mercedes Blázquez‐Sánchez, Mario Ruiz‐Serrano, María Jesús Muñoz, Patricia Rodríguez‐Sánchez, Belén Microb Biotechnol Research Articles Identification of Nocardia and Mycobacterium species by matrix‐assisted laser desorption/ionization‐time of flight mass spectrometry (MALDI‐TOF MS) is still a challenging task that requires both suitable protein extraction procedures and extensive databases. This study aimed to evaluate the VITEK MS Plus system coupled with updated RUO (v4.17) and IVD (v3.2) databases for the identification of Nocardia spp. and Mycobacterium spp. clinical isolates. Sample preparation was carried out using the VITEK MS Mycobacterium/Nocardia kit for protein extraction. From 90 Nocardia spp. isolates analysed, 86 (95.6%) were correctly identified at species or complex level using IVD and 78 (86.7%) using RUO. Only two strains were misidentified as other species pertaining to the same complex. Among the 106 non‐tuberculous Mycobacterium clinical isolates tested from a liquid culture medium, VITEK MS identified correctly at species or complex level 96 (90.6%) isolates in the IVD mode and 89 (84.0%) isolates in the RUO mode. No misidentifications were detected. Although the IVD mode was unable to differentiate members of the M. fortuitum complex, the RUO mode correctly discriminated M. peregrinum and M. septicum. The robustness and accuracy showed by this system allow its implementation for routine identification of these microorganisms in clinical laboratories. John Wiley and Sons Inc. 2022-12-20 /pmc/articles/PMC10034632/ /pubmed/36541026 http://dx.doi.org/10.1111/1751-7915.14146 Text en © 2022 The Authors. Microbial Biotechnology published by Society for Applied Microbiology and John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Rodríguez‐Temporal, David
Zvezdánova, Margarita Estreya
Benedí, Pablo
Marín, Mercedes
Blázquez‐Sánchez, Mario
Ruiz‐Serrano, María Jesús
Muñoz, Patricia
Rodríguez‐Sánchez, Belén
Identification of Nocardia and non‐tuberculous Mycobacterium species by MALDI‐TOF MS using the VITEK MS coupled to IVD and RUO databases
title Identification of Nocardia and non‐tuberculous Mycobacterium species by MALDI‐TOF MS using the VITEK MS coupled to IVD and RUO databases
title_full Identification of Nocardia and non‐tuberculous Mycobacterium species by MALDI‐TOF MS using the VITEK MS coupled to IVD and RUO databases
title_fullStr Identification of Nocardia and non‐tuberculous Mycobacterium species by MALDI‐TOF MS using the VITEK MS coupled to IVD and RUO databases
title_full_unstemmed Identification of Nocardia and non‐tuberculous Mycobacterium species by MALDI‐TOF MS using the VITEK MS coupled to IVD and RUO databases
title_short Identification of Nocardia and non‐tuberculous Mycobacterium species by MALDI‐TOF MS using the VITEK MS coupled to IVD and RUO databases
title_sort identification of nocardia and non‐tuberculous mycobacterium species by maldi‐tof ms using the vitek ms coupled to ivd and ruo databases
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10034632/
https://www.ncbi.nlm.nih.gov/pubmed/36541026
http://dx.doi.org/10.1111/1751-7915.14146
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