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On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum

Polygonum viviparum L. (PV) is a widely used resource plant with high medicinal, feeding and ecological values. Our studies show that PV has strong antioxidant activity. However, up to date, the antioxidant activity and components in other parts were not fully elucidated. In the present study, a new...

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Autores principales: Qian, Zheng-ming, Cheng, Xin-jie, Wang, Qiao, Huang, Qi, Jin, Li-ling, Ma, Ya-fei, Xie, Jia-sheng, Li, De-qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035567/
https://www.ncbi.nlm.nih.gov/pubmed/36968051
http://dx.doi.org/10.1039/d2ra08247k
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author Qian, Zheng-ming
Cheng, Xin-jie
Wang, Qiao
Huang, Qi
Jin, Li-ling
Ma, Ya-fei
Xie, Jia-sheng
Li, De-qiang
author_facet Qian, Zheng-ming
Cheng, Xin-jie
Wang, Qiao
Huang, Qi
Jin, Li-ling
Ma, Ya-fei
Xie, Jia-sheng
Li, De-qiang
author_sort Qian, Zheng-ming
collection PubMed
description Polygonum viviparum L. (PV) is a widely used resource plant with high medicinal, feeding and ecological values. Our studies show that PV has strong antioxidant activity. However, up to date, the antioxidant activity and components in other parts were not fully elucidated. In the present study, a new online pre-column ferric ion reducing antioxidant power (FRAP)-based antioxidant reaction coupled with high performance liquid chromatography-diode array detector-quadrupole-time-of-flight mass spectrometry (HPLC-DAD-TOF/MS) was developed for rapid and high-throughput screening of natural antioxidants from three different parts of PV including stems and leaves, fruits and rhizomes. In this procedure, it was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be greatly diminished or vanish after incubating with the FRAP. The online incubation conditions including mixed ratios of sample and FRAP solution and reaction times were firstly optimized with six standards. Then, the repeatability of the screening system was evaluated by analysis of the samples of stems and leaves of PV. As a result, a total of 21 compounds mainly including flavonoids and phenolic acids were screened from the three parts of PV. In conclusion, the present study provided a simple and effective strategy to rapidly screen antioxidants in natural products.
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spelling pubmed-100355672023-03-24 On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum Qian, Zheng-ming Cheng, Xin-jie Wang, Qiao Huang, Qi Jin, Li-ling Ma, Ya-fei Xie, Jia-sheng Li, De-qiang RSC Adv Chemistry Polygonum viviparum L. (PV) is a widely used resource plant with high medicinal, feeding and ecological values. Our studies show that PV has strong antioxidant activity. However, up to date, the antioxidant activity and components in other parts were not fully elucidated. In the present study, a new online pre-column ferric ion reducing antioxidant power (FRAP)-based antioxidant reaction coupled with high performance liquid chromatography-diode array detector-quadrupole-time-of-flight mass spectrometry (HPLC-DAD-TOF/MS) was developed for rapid and high-throughput screening of natural antioxidants from three different parts of PV including stems and leaves, fruits and rhizomes. In this procedure, it was assumed that the peak areas of compounds with potential antioxidant activity in HPLC chromatograms would be greatly diminished or vanish after incubating with the FRAP. The online incubation conditions including mixed ratios of sample and FRAP solution and reaction times were firstly optimized with six standards. Then, the repeatability of the screening system was evaluated by analysis of the samples of stems and leaves of PV. As a result, a total of 21 compounds mainly including flavonoids and phenolic acids were screened from the three parts of PV. In conclusion, the present study provided a simple and effective strategy to rapidly screen antioxidants in natural products. The Royal Society of Chemistry 2023-03-23 /pmc/articles/PMC10035567/ /pubmed/36968051 http://dx.doi.org/10.1039/d2ra08247k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Qian, Zheng-ming
Cheng, Xin-jie
Wang, Qiao
Huang, Qi
Jin, Li-ling
Ma, Ya-fei
Xie, Jia-sheng
Li, De-qiang
On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum
title On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum
title_full On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum
title_fullStr On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum
title_full_unstemmed On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum
title_short On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum
title_sort on-line pre-column frap-based antioxidant reaction coupled with hplc-dad-tof/ms for rapid screening of natural antioxidants from different parts of polygonum viviparum
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035567/
https://www.ncbi.nlm.nih.gov/pubmed/36968051
http://dx.doi.org/10.1039/d2ra08247k
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