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Circulating microRNAs as potential biomarkers of early vascular damage in vitamin D deficiency, obese, and diabetic patients

Vitamin D3 deficiency, obesity, and diabetes mellitus (DM) have been shown to increase the risk of cardiovascular diseases (CVDs). However, the early detection of vascular damage in those patients is still difficult to ascertain. MicroRNAs (miRNAs) are recognized to play a critical role in initiatio...

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Autores principales: Elmoselhi, Adel B., Seif Allah, Mohamed, Bouzid, Amal, Ibrahim, Zeinab, Venkatachalam, Thenmozhi, Siddiqui, Ruqaiyyah, Khan, Naveed Ahmed, Hamoudi, Rifat A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035929/
https://www.ncbi.nlm.nih.gov/pubmed/36952563
http://dx.doi.org/10.1371/journal.pone.0283608
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author Elmoselhi, Adel B.
Seif Allah, Mohamed
Bouzid, Amal
Ibrahim, Zeinab
Venkatachalam, Thenmozhi
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
Hamoudi, Rifat A.
author_facet Elmoselhi, Adel B.
Seif Allah, Mohamed
Bouzid, Amal
Ibrahim, Zeinab
Venkatachalam, Thenmozhi
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
Hamoudi, Rifat A.
author_sort Elmoselhi, Adel B.
collection PubMed
description Vitamin D3 deficiency, obesity, and diabetes mellitus (DM) have been shown to increase the risk of cardiovascular diseases (CVDs). However, the early detection of vascular damage in those patients is still difficult to ascertain. MicroRNAs (miRNAs) are recognized to play a critical role in initiation and pathogenesis of vascular dysfunction. Herein, we aimed to identify circulating miRNA biomarkers of vascular dysfunction as early predictors of CVDs. We have recruited 23 middle-aged Emiratis patients with the following criteria: A healthy control group with vitamin D ≥ 20ng, and BMI < 30 (C1 group = 11 individuals); A vitamin D deficiency (Vit D level ≤ 20 ng) and obese (BMI ≥ 30) group (A1 group = 9 patients); A vitamin D deficiency, obese, plus DM (A2 group = 3 patients). Arterial stiffness via pulse wave velocity (PWV) was measured and the whole transcriptome analysis with qPCR validation for miRNA in plasma samples were tested. PWV relative to age was significantly higher in A1 group 19.4 ± 4.7 m/s and A2 group 18.3 ± 1.3 m/s compared to controls 14.7 ± 2.1 m/s (p < 0.05). Similar patterns were also observed in the Augmentation pressure (AP) and Alx%. Whole RNA-Sequencing revealed miR-182-5p; miR-199a-5p; miR-193a-5p; and miR-155-5p were differentially over-expressed (logFC > 1.5) in high-risk patients for CVDs vs healthy controls. Collectively, our result indicates that four specific circulating miRNA signature, may be utilized as non-invasive, diagnostic and prognostic biomarkers for early vascular damage in patients suffering from vitamin D deficiency, obesity and DM.
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spelling pubmed-100359292023-03-24 Circulating microRNAs as potential biomarkers of early vascular damage in vitamin D deficiency, obese, and diabetic patients Elmoselhi, Adel B. Seif Allah, Mohamed Bouzid, Amal Ibrahim, Zeinab Venkatachalam, Thenmozhi Siddiqui, Ruqaiyyah Khan, Naveed Ahmed Hamoudi, Rifat A. PLoS One Research Article Vitamin D3 deficiency, obesity, and diabetes mellitus (DM) have been shown to increase the risk of cardiovascular diseases (CVDs). However, the early detection of vascular damage in those patients is still difficult to ascertain. MicroRNAs (miRNAs) are recognized to play a critical role in initiation and pathogenesis of vascular dysfunction. Herein, we aimed to identify circulating miRNA biomarkers of vascular dysfunction as early predictors of CVDs. We have recruited 23 middle-aged Emiratis patients with the following criteria: A healthy control group with vitamin D ≥ 20ng, and BMI < 30 (C1 group = 11 individuals); A vitamin D deficiency (Vit D level ≤ 20 ng) and obese (BMI ≥ 30) group (A1 group = 9 patients); A vitamin D deficiency, obese, plus DM (A2 group = 3 patients). Arterial stiffness via pulse wave velocity (PWV) was measured and the whole transcriptome analysis with qPCR validation for miRNA in plasma samples were tested. PWV relative to age was significantly higher in A1 group 19.4 ± 4.7 m/s and A2 group 18.3 ± 1.3 m/s compared to controls 14.7 ± 2.1 m/s (p < 0.05). Similar patterns were also observed in the Augmentation pressure (AP) and Alx%. Whole RNA-Sequencing revealed miR-182-5p; miR-199a-5p; miR-193a-5p; and miR-155-5p were differentially over-expressed (logFC > 1.5) in high-risk patients for CVDs vs healthy controls. Collectively, our result indicates that four specific circulating miRNA signature, may be utilized as non-invasive, diagnostic and prognostic biomarkers for early vascular damage in patients suffering from vitamin D deficiency, obesity and DM. Public Library of Science 2023-03-23 /pmc/articles/PMC10035929/ /pubmed/36952563 http://dx.doi.org/10.1371/journal.pone.0283608 Text en © 2023 Elmoselhi et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Elmoselhi, Adel B.
Seif Allah, Mohamed
Bouzid, Amal
Ibrahim, Zeinab
Venkatachalam, Thenmozhi
Siddiqui, Ruqaiyyah
Khan, Naveed Ahmed
Hamoudi, Rifat A.
Circulating microRNAs as potential biomarkers of early vascular damage in vitamin D deficiency, obese, and diabetic patients
title Circulating microRNAs as potential biomarkers of early vascular damage in vitamin D deficiency, obese, and diabetic patients
title_full Circulating microRNAs as potential biomarkers of early vascular damage in vitamin D deficiency, obese, and diabetic patients
title_fullStr Circulating microRNAs as potential biomarkers of early vascular damage in vitamin D deficiency, obese, and diabetic patients
title_full_unstemmed Circulating microRNAs as potential biomarkers of early vascular damage in vitamin D deficiency, obese, and diabetic patients
title_short Circulating microRNAs as potential biomarkers of early vascular damage in vitamin D deficiency, obese, and diabetic patients
title_sort circulating micrornas as potential biomarkers of early vascular damage in vitamin d deficiency, obese, and diabetic patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035929/
https://www.ncbi.nlm.nih.gov/pubmed/36952563
http://dx.doi.org/10.1371/journal.pone.0283608
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