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KRT13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance
Pancreatic cancer is one of the most aggressive cancers and the seventh leading cause of cancer-associated death in the world. Radiation is performed as an adjuvant therapy as well as anti-cancer drugs. Because cancer stem-like cells (CSCs) are considered to be radioresistant and cause recurrence an...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036105/ https://www.ncbi.nlm.nih.gov/pubmed/36610719 http://dx.doi.org/10.1093/jrr/rrac091 |
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author | Takenaka, Wataru Yokoyama, Yuhki Ikehata, Katsuya Kouda, Shihori Hirose, Haruka Minami, Kazumasa Hamada, Yoshinosuke Mori, Seiji Koizumi, Masahiko Yamamoto, Hirofumi |
author_facet | Takenaka, Wataru Yokoyama, Yuhki Ikehata, Katsuya Kouda, Shihori Hirose, Haruka Minami, Kazumasa Hamada, Yoshinosuke Mori, Seiji Koizumi, Masahiko Yamamoto, Hirofumi |
author_sort | Takenaka, Wataru |
collection | PubMed |
description | Pancreatic cancer is one of the most aggressive cancers and the seventh leading cause of cancer-associated death in the world. Radiation is performed as an adjuvant therapy as well as anti-cancer drugs. Because cancer stem-like cells (CSCs) are considered to be radioresistant and cause recurrence and metastasis, understanding their properties is required for the development of novel therapeutic strategies. To investigate the CSC properties of pancreatic cancer cells, we used a pancreatic CSC model, degron (++) cells, which have low proteasome activity. Degron (++) cells displayed radioresistance in comparison with control cells. Using Ribonucleic acid (RNA) sequencing, we successfully identified KRT13 as a candidate gene responsible for radioresistance. Knockdown of KRT13 sensitized the degron (++) cells to radiation. Furthermore, a database search revealed that KRT13 is upregulated in pancreatic cancer cell lines and that high expression of KRT13 is associated with poorer prognosis. These results indicate that a combination therapy of KRT13 knockdown and radiation could hold therapeutic promise in pancreatic cancer. |
format | Online Article Text |
id | pubmed-10036105 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-100361052023-03-24 KRT13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance Takenaka, Wataru Yokoyama, Yuhki Ikehata, Katsuya Kouda, Shihori Hirose, Haruka Minami, Kazumasa Hamada, Yoshinosuke Mori, Seiji Koizumi, Masahiko Yamamoto, Hirofumi J Radiat Res Regular Paper Pancreatic cancer is one of the most aggressive cancers and the seventh leading cause of cancer-associated death in the world. Radiation is performed as an adjuvant therapy as well as anti-cancer drugs. Because cancer stem-like cells (CSCs) are considered to be radioresistant and cause recurrence and metastasis, understanding their properties is required for the development of novel therapeutic strategies. To investigate the CSC properties of pancreatic cancer cells, we used a pancreatic CSC model, degron (++) cells, which have low proteasome activity. Degron (++) cells displayed radioresistance in comparison with control cells. Using Ribonucleic acid (RNA) sequencing, we successfully identified KRT13 as a candidate gene responsible for radioresistance. Knockdown of KRT13 sensitized the degron (++) cells to radiation. Furthermore, a database search revealed that KRT13 is upregulated in pancreatic cancer cell lines and that high expression of KRT13 is associated with poorer prognosis. These results indicate that a combination therapy of KRT13 knockdown and radiation could hold therapeutic promise in pancreatic cancer. Oxford University Press 2023-01-04 /pmc/articles/PMC10036105/ /pubmed/36610719 http://dx.doi.org/10.1093/jrr/rrac091 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of The Japanese Radiation Research Society and Japanese Society for Radiation Oncology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Paper Takenaka, Wataru Yokoyama, Yuhki Ikehata, Katsuya Kouda, Shihori Hirose, Haruka Minami, Kazumasa Hamada, Yoshinosuke Mori, Seiji Koizumi, Masahiko Yamamoto, Hirofumi KRT13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance |
title |
KRT13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance |
title_full |
KRT13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance |
title_fullStr |
KRT13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance |
title_full_unstemmed |
KRT13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance |
title_short |
KRT13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance |
title_sort | krt13 is upregulated in pancreatic cancer stem-like cells and associated with radioresistance |
topic | Regular Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036105/ https://www.ncbi.nlm.nih.gov/pubmed/36610719 http://dx.doi.org/10.1093/jrr/rrac091 |
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