SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests

Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and f...

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Autores principales: Nguyen, Phuong-Vi, Carmola, Ludy Registre, Wang, Ethan, Bassit, Leda, Rao, Anuradha, Greenleaf, Morgan, Sullivan, Julie A., Martin, Greg S., Lam, Wilbur A., Waggoner, Jesse J., Piantadosi, Anne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Author(s). Published by Elsevier B.V. 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036152/
https://www.ncbi.nlm.nih.gov/pubmed/37028004
http://dx.doi.org/10.1016/j.jcv.2023.105426
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author Nguyen, Phuong-Vi
Carmola, Ludy Registre
Wang, Ethan
Bassit, Leda
Rao, Anuradha
Greenleaf, Morgan
Sullivan, Julie A.
Martin, Greg S.
Lam, Wilbur A.
Waggoner, Jesse J.
Piantadosi, Anne
author_facet Nguyen, Phuong-Vi
Carmola, Ludy Registre
Wang, Ethan
Bassit, Leda
Rao, Anuradha
Greenleaf, Morgan
Sullivan, Julie A.
Martin, Greg S.
Lam, Wilbur A.
Waggoner, Jesse J.
Piantadosi, Anne
author_sort Nguyen, Phuong-Vi
collection PubMed
description Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution.
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spelling pubmed-100361522023-03-24 SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests Nguyen, Phuong-Vi Carmola, Ludy Registre Wang, Ethan Bassit, Leda Rao, Anuradha Greenleaf, Morgan Sullivan, Julie A. Martin, Greg S. Lam, Wilbur A. Waggoner, Jesse J. Piantadosi, Anne J Clin Virol Article Widespread use of over-the-counter rapid diagnostic tests for SARS-CoV-2 has led to a decrease in availability of clinical samples for viral genomic surveillance. As an alternative sample source, we evaluated RNA isolated from BinaxNOW swabs stored at ambient temperature for SARS-CoV-2 rRT-PCR and full viral genome sequencing. 81 of 103 samples (78.6%) yielded detectable RNA, and 46 of 57 samples (80.7 %) yielded complete genome sequences. Our results illustrate that SARS-CoV-2 RNA extracted from used Binax test swabs provides an important opportunity for improving SARS-CoV-2 genomic surveillance, evaluating transmission clusters, and monitoring within-patient evolution. The Author(s). Published by Elsevier B.V. 2023-05 2023-03-24 /pmc/articles/PMC10036152/ /pubmed/37028004 http://dx.doi.org/10.1016/j.jcv.2023.105426 Text en © 2023 The Author(s) Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Nguyen, Phuong-Vi
Carmola, Ludy Registre
Wang, Ethan
Bassit, Leda
Rao, Anuradha
Greenleaf, Morgan
Sullivan, Julie A.
Martin, Greg S.
Lam, Wilbur A.
Waggoner, Jesse J.
Piantadosi, Anne
SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests
title SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests
title_full SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests
title_fullStr SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests
title_full_unstemmed SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests
title_short SARS-CoV-2 molecular testing and whole genome sequencing following RNA recovery from used BinaxNOW COVID-19 antigen self tests
title_sort sars-cov-2 molecular testing and whole genome sequencing following rna recovery from used binaxnow covid-19 antigen self tests
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036152/
https://www.ncbi.nlm.nih.gov/pubmed/37028004
http://dx.doi.org/10.1016/j.jcv.2023.105426
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