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Normalizing salt content by mixing native human airway mucus samples normalizes sample rheology
Across the globe, millions of people are affected by muco-obstructive pulmonary diseases like cystic fibrosis, asthma, and chronic obstructive pulmonary disease. In MOPDs, the airway mucus becomes hyperconcentrated, increasing viscoelasticity and impairing mucus clearance. Research focused on treatm...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036356/ https://www.ncbi.nlm.nih.gov/pubmed/36969580 http://dx.doi.org/10.3389/fphys.2023.1111647 |
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author | Markovetz, Matthew R. Hibbard, Jacob E. Plott, Lucas M. Bacudio, Lawrence G. Kissner, William J. Ghio, Andrew Kumar, Priya A. Arora, Harendra Hill, David B. |
author_facet | Markovetz, Matthew R. Hibbard, Jacob E. Plott, Lucas M. Bacudio, Lawrence G. Kissner, William J. Ghio, Andrew Kumar, Priya A. Arora, Harendra Hill, David B. |
author_sort | Markovetz, Matthew R. |
collection | PubMed |
description | Across the globe, millions of people are affected by muco-obstructive pulmonary diseases like cystic fibrosis, asthma, and chronic obstructive pulmonary disease. In MOPDs, the airway mucus becomes hyperconcentrated, increasing viscoelasticity and impairing mucus clearance. Research focused on treatment of MOPDs requires relevant sources of airway mucus both as a control sample type and as a basis for manipulation to study the effects of additional hyperconcentration, inflammatory milieu, and biofilm growth on the biochemical and biophysical properties of mucus. Endotracheal tube mucus has been identified as a prospective source of native airway mucus given its several advantages over sputum and airway cell culture mucus such as ease of access and in vivo production that includes surface airway and submucosal gland secretions. Still, many ETT samples suffer from altered tonicity and composition from either dehydration, salivary dilution, or other contamination. Herein, the biochemical compositions of ETT mucus from healthy human subjects were determined. Samples were characterized in terms of tonicity, pooled, and restored to normal tonicity. Salt-normalized ETT mucus exhibited similar concentration-dependent rheologic properties as originally isotonic mucus. This rheology agreed across spatial scales and with previous reports of the biophysics of ETT mucus. This work affirms previous reports of the importance of salt concentration on mucus rheology and presents methodology to increase yield native airway mucus samples for laboratory use and manipulation. |
format | Online Article Text |
id | pubmed-10036356 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-100363562023-03-25 Normalizing salt content by mixing native human airway mucus samples normalizes sample rheology Markovetz, Matthew R. Hibbard, Jacob E. Plott, Lucas M. Bacudio, Lawrence G. Kissner, William J. Ghio, Andrew Kumar, Priya A. Arora, Harendra Hill, David B. Front Physiol Physiology Across the globe, millions of people are affected by muco-obstructive pulmonary diseases like cystic fibrosis, asthma, and chronic obstructive pulmonary disease. In MOPDs, the airway mucus becomes hyperconcentrated, increasing viscoelasticity and impairing mucus clearance. Research focused on treatment of MOPDs requires relevant sources of airway mucus both as a control sample type and as a basis for manipulation to study the effects of additional hyperconcentration, inflammatory milieu, and biofilm growth on the biochemical and biophysical properties of mucus. Endotracheal tube mucus has been identified as a prospective source of native airway mucus given its several advantages over sputum and airway cell culture mucus such as ease of access and in vivo production that includes surface airway and submucosal gland secretions. Still, many ETT samples suffer from altered tonicity and composition from either dehydration, salivary dilution, or other contamination. Herein, the biochemical compositions of ETT mucus from healthy human subjects were determined. Samples were characterized in terms of tonicity, pooled, and restored to normal tonicity. Salt-normalized ETT mucus exhibited similar concentration-dependent rheologic properties as originally isotonic mucus. This rheology agreed across spatial scales and with previous reports of the biophysics of ETT mucus. This work affirms previous reports of the importance of salt concentration on mucus rheology and presents methodology to increase yield native airway mucus samples for laboratory use and manipulation. Frontiers Media S.A. 2023-03-10 /pmc/articles/PMC10036356/ /pubmed/36969580 http://dx.doi.org/10.3389/fphys.2023.1111647 Text en Copyright © 2023 Markovetz, Hibbard, Plott, Bacudio, Kissner, Ghio, Kumar, Arora and Hill. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Markovetz, Matthew R. Hibbard, Jacob E. Plott, Lucas M. Bacudio, Lawrence G. Kissner, William J. Ghio, Andrew Kumar, Priya A. Arora, Harendra Hill, David B. Normalizing salt content by mixing native human airway mucus samples normalizes sample rheology |
title | Normalizing salt content by mixing native human airway mucus samples normalizes sample rheology |
title_full | Normalizing salt content by mixing native human airway mucus samples normalizes sample rheology |
title_fullStr | Normalizing salt content by mixing native human airway mucus samples normalizes sample rheology |
title_full_unstemmed | Normalizing salt content by mixing native human airway mucus samples normalizes sample rheology |
title_short | Normalizing salt content by mixing native human airway mucus samples normalizes sample rheology |
title_sort | normalizing salt content by mixing native human airway mucus samples normalizes sample rheology |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036356/ https://www.ncbi.nlm.nih.gov/pubmed/36969580 http://dx.doi.org/10.3389/fphys.2023.1111647 |
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