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Estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice
Decreased estrogens during menopause are associated with increased risk of anxiety, depression, type 2 diabetes and obesity. Similarly, depleting estrogens in rodents by ovariectomy, combined with a high-fat diet (HFD), increases anxiety and adiposity. How estrogens and diet interact to affect anxie...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036463/ https://www.ncbi.nlm.nih.gov/pubmed/36959275 http://dx.doi.org/10.1038/s41598-023-31783-6 |
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author | Acharya, Kalpana D. Graham, Madeline Raman, Harshini Parakoyi, Abigail E. R. Corcoran, Alexis Belete, Merzu Ramaswamy, Bharath Koul, Shashikant Sachar, Ishneet Derendorf, Kevin Wilmer, Jeremy B. Gottipati, Srikanth Tetel, Marc J. |
author_facet | Acharya, Kalpana D. Graham, Madeline Raman, Harshini Parakoyi, Abigail E. R. Corcoran, Alexis Belete, Merzu Ramaswamy, Bharath Koul, Shashikant Sachar, Ishneet Derendorf, Kevin Wilmer, Jeremy B. Gottipati, Srikanth Tetel, Marc J. |
author_sort | Acharya, Kalpana D. |
collection | PubMed |
description | Decreased estrogens during menopause are associated with increased risk of anxiety, depression, type 2 diabetes and obesity. Similarly, depleting estrogens in rodents by ovariectomy, combined with a high-fat diet (HFD), increases anxiety and adiposity. How estrogens and diet interact to affect anxiety and metabolism is poorly understood. Mounting evidence indicates that gut microbiota influence anxiety and metabolism. Here, we investigated the effects of estradiol (E) and HFD on anxiety, metabolism, and their correlation with changes in gut microbiota in female mice. Adult C57BL/6J mice were ovariectomized, implanted with E or vehicle-containing capsules and fed a standard diet or HFD. Anxiety-like behavior was assessed and neuronal activation was measured by c-fos immunoreactivity throughout the brain using iDISCO. HFD increased anxiety-like behavior, while E reduced this HFD-dependent anxiogenic effect. Interestingly, E decreased neuronal activation in brain regions involved in anxiety and metabolism. E treatment also altered gut microbes, a subset of which were associated with anxiety-like behavior. These findings provide insight into gut microbiota-based therapies for anxiety and metabolic disorders associated with declining estrogens in menopausal women. |
format | Online Article Text |
id | pubmed-10036463 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-100364632023-03-25 Estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice Acharya, Kalpana D. Graham, Madeline Raman, Harshini Parakoyi, Abigail E. R. Corcoran, Alexis Belete, Merzu Ramaswamy, Bharath Koul, Shashikant Sachar, Ishneet Derendorf, Kevin Wilmer, Jeremy B. Gottipati, Srikanth Tetel, Marc J. Sci Rep Article Decreased estrogens during menopause are associated with increased risk of anxiety, depression, type 2 diabetes and obesity. Similarly, depleting estrogens in rodents by ovariectomy, combined with a high-fat diet (HFD), increases anxiety and adiposity. How estrogens and diet interact to affect anxiety and metabolism is poorly understood. Mounting evidence indicates that gut microbiota influence anxiety and metabolism. Here, we investigated the effects of estradiol (E) and HFD on anxiety, metabolism, and their correlation with changes in gut microbiota in female mice. Adult C57BL/6J mice were ovariectomized, implanted with E or vehicle-containing capsules and fed a standard diet or HFD. Anxiety-like behavior was assessed and neuronal activation was measured by c-fos immunoreactivity throughout the brain using iDISCO. HFD increased anxiety-like behavior, while E reduced this HFD-dependent anxiogenic effect. Interestingly, E decreased neuronal activation in brain regions involved in anxiety and metabolism. E treatment also altered gut microbes, a subset of which were associated with anxiety-like behavior. These findings provide insight into gut microbiota-based therapies for anxiety and metabolic disorders associated with declining estrogens in menopausal women. Nature Publishing Group UK 2023-03-23 /pmc/articles/PMC10036463/ /pubmed/36959275 http://dx.doi.org/10.1038/s41598-023-31783-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Acharya, Kalpana D. Graham, Madeline Raman, Harshini Parakoyi, Abigail E. R. Corcoran, Alexis Belete, Merzu Ramaswamy, Bharath Koul, Shashikant Sachar, Ishneet Derendorf, Kevin Wilmer, Jeremy B. Gottipati, Srikanth Tetel, Marc J. Estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice |
title | Estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice |
title_full | Estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice |
title_fullStr | Estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice |
title_full_unstemmed | Estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice |
title_short | Estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice |
title_sort | estradiol-mediated protection against high-fat diet induced anxiety and obesity is associated with changes in the gut microbiota in female mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036463/ https://www.ncbi.nlm.nih.gov/pubmed/36959275 http://dx.doi.org/10.1038/s41598-023-31783-6 |
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