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Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications

Rhodotorula toruloides is a non-conventional yeast with a natural carotenoid pathway. In particular, R. toruloides is an oleaginous yeast that can accumulate lipids in high content, thereby gaining interest as a promising industrial host. In this study, we isolated and taxonomically identified a new...

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Autores principales: Kim, Junyeob, Lee, Eun-Jung, Lee, Kyung-Eun, Nho, Youn-Hwa, Ryu, Jeoungjin, Kim, Su Young, Yoo, Jeong Kyun, Kang, Seunghyun, Seo, Sang Woo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Research Network of Computational and Structural Biotechnology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036517/
https://www.ncbi.nlm.nih.gov/pubmed/36968014
http://dx.doi.org/10.1016/j.csbj.2023.03.018
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author Kim, Junyeob
Lee, Eun-Jung
Lee, Kyung-Eun
Nho, Youn-Hwa
Ryu, Jeoungjin
Kim, Su Young
Yoo, Jeong Kyun
Kang, Seunghyun
Seo, Sang Woo
author_facet Kim, Junyeob
Lee, Eun-Jung
Lee, Kyung-Eun
Nho, Youn-Hwa
Ryu, Jeoungjin
Kim, Su Young
Yoo, Jeong Kyun
Kang, Seunghyun
Seo, Sang Woo
author_sort Kim, Junyeob
collection PubMed
description Rhodotorula toruloides is a non-conventional yeast with a natural carotenoid pathway. In particular, R. toruloides is an oleaginous yeast that can accumulate lipids in high content, thereby gaining interest as a promising industrial host. In this study, we isolated and taxonomically identified a new R. toruloides LAB-07 strain. De novo genome assembly using PacBio and Illumina hybrid platforms yielded 27 contigs with a 20.78 Mb genome size. Subsequent genome annotation analysis based on RNA-seq predicted 5296 protein-coding genes, including the fatty acid production pathway. We compared lipid production under different media; it was highest in the yeast extract salt medium with glycerol as a carbon source. Polyunsaturated α-linolenic acid was detected among the fatty acids, and docking phosphatidylcholine as a substrate to modeled Fad2, which annotated as Δ12-fatty acid desaturase showed bifunctional Δ12, 15-desaturation is structurally possible in that the distances between the diiron center and the carbon-carbon bond in which desaturation occurs were similar to those of structurally identified mouse stearoyl-CoA desaturase. Finally, the applicability of the extracted total lipid fraction of R. toruloides was investigated, demonstrating an increase in filaggrin expression and suppression of heat-induced MMP-1 expression when applied to keratinocytes, along with the additional antioxidant activity. This work presents a new R. toruloides LAB-07 strain with genomic and lipidomic data, which would help understand the physiology of R. toruloides. Also, the various skin-related effect of R. toruloides lipid extract indicates its potential usage as a promising cosmetic ingredient.
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spelling pubmed-100365172023-03-25 Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications Kim, Junyeob Lee, Eun-Jung Lee, Kyung-Eun Nho, Youn-Hwa Ryu, Jeoungjin Kim, Su Young Yoo, Jeong Kyun Kang, Seunghyun Seo, Sang Woo Comput Struct Biotechnol J Research Article Rhodotorula toruloides is a non-conventional yeast with a natural carotenoid pathway. In particular, R. toruloides is an oleaginous yeast that can accumulate lipids in high content, thereby gaining interest as a promising industrial host. In this study, we isolated and taxonomically identified a new R. toruloides LAB-07 strain. De novo genome assembly using PacBio and Illumina hybrid platforms yielded 27 contigs with a 20.78 Mb genome size. Subsequent genome annotation analysis based on RNA-seq predicted 5296 protein-coding genes, including the fatty acid production pathway. We compared lipid production under different media; it was highest in the yeast extract salt medium with glycerol as a carbon source. Polyunsaturated α-linolenic acid was detected among the fatty acids, and docking phosphatidylcholine as a substrate to modeled Fad2, which annotated as Δ12-fatty acid desaturase showed bifunctional Δ12, 15-desaturation is structurally possible in that the distances between the diiron center and the carbon-carbon bond in which desaturation occurs were similar to those of structurally identified mouse stearoyl-CoA desaturase. Finally, the applicability of the extracted total lipid fraction of R. toruloides was investigated, demonstrating an increase in filaggrin expression and suppression of heat-induced MMP-1 expression when applied to keratinocytes, along with the additional antioxidant activity. This work presents a new R. toruloides LAB-07 strain with genomic and lipidomic data, which would help understand the physiology of R. toruloides. Also, the various skin-related effect of R. toruloides lipid extract indicates its potential usage as a promising cosmetic ingredient. Research Network of Computational and Structural Biotechnology 2023-03-13 /pmc/articles/PMC10036517/ /pubmed/36968014 http://dx.doi.org/10.1016/j.csbj.2023.03.018 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Kim, Junyeob
Lee, Eun-Jung
Lee, Kyung-Eun
Nho, Youn-Hwa
Ryu, Jeoungjin
Kim, Su Young
Yoo, Jeong Kyun
Kang, Seunghyun
Seo, Sang Woo
Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications
title Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications
title_full Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications
title_fullStr Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications
title_full_unstemmed Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications
title_short Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications
title_sort lipid extract derived from newly isolated rhodotorula toruloides lab-07 for cosmetic applications
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036517/
https://www.ncbi.nlm.nih.gov/pubmed/36968014
http://dx.doi.org/10.1016/j.csbj.2023.03.018
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