Establishment and Characterization of an Acute Lymphocytic Leukemia Cell Line Expressing CD13 and CD33 with a Complex Philadelphia Translocation

OBJECTIVE: To investigate the pathogenesis of Philadelphia (Ph)-positive acute lymphocytic leukemia (ALL), we established a lymphoblastoid cell line. METHODS: Bone marrow cells from a patient with Ph-positive ALL were enriched by Ficoll-Hypaque centrifugation and cultured in medium with fetal calf s...

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Detalles Bibliográficos
Autores principales: Sameshima, Yuichi, Okada, Michiko, Shiseki, Masayuki, Mori, Naoki, Motoji, Toshiko, Tanaka, Junji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Internal Medicine 2023
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10036999/
https://www.ncbi.nlm.nih.gov/pubmed/36858620
http://dx.doi.org/10.2169/internalmedicine.9359-22
Descripción
Sumario:OBJECTIVE: To investigate the pathogenesis of Philadelphia (Ph)-positive acute lymphocytic leukemia (ALL), we established a lymphoblastoid cell line. METHODS: Bone marrow cells from a patient with Ph-positive ALL were enriched by Ficoll-Hypaque centrifugation and cultured in medium with fetal calf serum. MATERIALS: The mononuclear cells of bone marrow aspirate were obtained from an adult man with ALL after he experienced relapse following induction therapy including imatinib mesylate. RESULTS: The cell line termed TNA-M was established, carrying a three-way Ph translocation involving two chromosome 9s and one chromosome 22 as a sole karyotypic abnormality. Furthermore, the cells were positive for CD13 and CD33 in addition to CD19, CD22 and CD79a antigens. CONCLUSION: This unique cell line is expected to be a valuable tool for understanding the pathogenesis of Ph-positive ALL.

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