A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample

We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h,...

Descripción completa

Detalles Bibliográficos
Autores principales: Okazaki, Yusuke, Nguyen, Tuyen Thi, Nishihara, Arisa, Endo, Hisashi, Ogata, Hiroyuki, Nakano, Shin-ichi, Tamaki, Hideyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2023
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037101/
https://www.ncbi.nlm.nih.gov/pubmed/36928278
http://dx.doi.org/10.1264/jsme2.ME22102
_version_ 1784911806344462336
author Okazaki, Yusuke
Nguyen, Tuyen Thi
Nishihara, Arisa
Endo, Hisashi
Ogata, Hiroyuki
Nakano, Shin-ichi
Tamaki, Hideyuki
author_facet Okazaki, Yusuke
Nguyen, Tuyen Thi
Nishihara, Arisa
Endo, Hisashi
Ogata, Hiroyuki
Nakano, Shin-ichi
Tamaki, Hideyuki
author_sort Okazaki, Yusuke
collection PubMed
description We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10–15‍ ‍kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50‍ ‍ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications.
format Online
Article
Text
id pubmed-10037101
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
record_format MEDLINE/PubMed
spelling pubmed-100371012023-03-25 A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample Okazaki, Yusuke Nguyen, Tuyen Thi Nishihara, Arisa Endo, Hisashi Ogata, Hiroyuki Nakano, Shin-ichi Tamaki, Hideyuki Microbes Environ Short Communication We herein propose a fast and easy DNA and RNA co-extraction method for environmental microbial samples. It combines bead beating and phenol-chloroform phase separation followed by the separation and purification of DNA and RNA using the Qiagen AllPrep DNA/RNA mini kit. With a handling time of ~3 h, our method simultaneously extracted high-quality DNA (peak size >10–15‍ ‍kb) and RNA (RNA integrity number >6) from lake bacterioplankton filtered samples. The method is also applicable to low-biomass samples (expected DNA or RNA yield <50‍ ‍ng) and eukaryotic microbial samples, providing an easy option for more versatile eco-genomic applications. Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles 2023 2023-03-15 /pmc/articles/PMC10037101/ /pubmed/36928278 http://dx.doi.org/10.1264/jsme2.ME22102 Text en 2023 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Okazaki, Yusuke
Nguyen, Tuyen Thi
Nishihara, Arisa
Endo, Hisashi
Ogata, Hiroyuki
Nakano, Shin-ichi
Tamaki, Hideyuki
A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample
title A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample
title_full A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample
title_fullStr A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample
title_full_unstemmed A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample
title_short A Fast and Easy Method to Co-extract DNA and RNA from an Environmental Microbial Sample
title_sort fast and easy method to co-extract dna and rna from an environmental microbial sample
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037101/
https://www.ncbi.nlm.nih.gov/pubmed/36928278
http://dx.doi.org/10.1264/jsme2.ME22102
work_keys_str_mv AT okazakiyusuke afastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT nguyentuyenthi afastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT nishiharaarisa afastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT endohisashi afastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT ogatahiroyuki afastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT nakanoshinichi afastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT tamakihideyuki afastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT okazakiyusuke fastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT nguyentuyenthi fastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT nishiharaarisa fastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT endohisashi fastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT ogatahiroyuki fastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT nakanoshinichi fastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample
AT tamakihideyuki fastandeasymethodtocoextractdnaandrnafromanenvironmentalmicrobialsample

Ejemplares similares