In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells

Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to induce tubu...

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Detalles Bibliográficos
Autores principales: Kraus, Andre, Lüdke, Andrea, Teschemacher, Barbara, Skoczynski, Kathrin, Buchholz, Bjoern
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037192/
https://www.ncbi.nlm.nih.gov/pubmed/36856763
http://dx.doi.org/10.1016/j.xpro.2022.101874
Descripción
Sumario:Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to induce tubule formation. We detail steps for the establishment of the in vitro cyst assay, followed by puncture of the cysts in the collagen matrix. We further describe live imaging and steps to analyze the tubule growth. For complete details on the use and execution of this protocol, please refer to Scholz et al. (2022).(1)

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