In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells

Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to induce tubu...

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Autores principales: Kraus, Andre, Lüdke, Andrea, Teschemacher, Barbara, Skoczynski, Kathrin, Buchholz, Bjoern
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037192/
https://www.ncbi.nlm.nih.gov/pubmed/36856763
http://dx.doi.org/10.1016/j.xpro.2022.101874
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author Kraus, Andre
Lüdke, Andrea
Teschemacher, Barbara
Skoczynski, Kathrin
Buchholz, Bjoern
author_facet Kraus, Andre
Lüdke, Andrea
Teschemacher, Barbara
Skoczynski, Kathrin
Buchholz, Bjoern
author_sort Kraus, Andre
collection PubMed
description Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to induce tubule formation. We detail steps for the establishment of the in vitro cyst assay, followed by puncture of the cysts in the collagen matrix. We further describe live imaging and steps to analyze the tubule growth. For complete details on the use and execution of this protocol, please refer to Scholz et al. (2022).(1)
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spelling pubmed-100371922023-03-25 In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells Kraus, Andre Lüdke, Andrea Teschemacher, Barbara Skoczynski, Kathrin Buchholz, Bjoern STAR Protoc Protocol Collecting-duct-derived renal epithelial cells switch from tubule to cyst formation; however, the cysts still form tubules after injury of the cyst-lining epithelium. Here, we provide a protocol that describes in vitro cyst growth with focus on glass-capillary-induced cyst wall injury to induce tubule formation. We detail steps for the establishment of the in vitro cyst assay, followed by puncture of the cysts in the collagen matrix. We further describe live imaging and steps to analyze the tubule growth. For complete details on the use and execution of this protocol, please refer to Scholz et al. (2022).(1) Elsevier 2023-01-19 /pmc/articles/PMC10037192/ /pubmed/36856763 http://dx.doi.org/10.1016/j.xpro.2022.101874 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kraus, Andre
Lüdke, Andrea
Teschemacher, Barbara
Skoczynski, Kathrin
Buchholz, Bjoern
In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
title In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
title_full In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
title_fullStr In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
title_full_unstemmed In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
title_short In vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
title_sort in vitro cyst puncture and injury-induced tubule formation using renal epithelial cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037192/
https://www.ncbi.nlm.nih.gov/pubmed/36856763
http://dx.doi.org/10.1016/j.xpro.2022.101874
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