Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models

Understanding the glioblastoma (GBM) immune microenvironment and development of clinical treatment drugs rely on suitable preclinical GBM models. Here, we present a protocol to establish syngeneic orthotopic glioma mouse models. We also describe the steps to intracranially deliver immunotherapeutic...

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Autores principales: Chen, Apeng, Chao, Yapeng, Zou, Han, McCarl, Lauren H., Kohanbash, Gary, Hu, Baoli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037209/
https://www.ncbi.nlm.nih.gov/pubmed/36861832
http://dx.doi.org/10.1016/j.xpro.2023.102049
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author Chen, Apeng
Chao, Yapeng
Zou, Han
McCarl, Lauren H.
Kohanbash, Gary
Hu, Baoli
author_facet Chen, Apeng
Chao, Yapeng
Zou, Han
McCarl, Lauren H.
Kohanbash, Gary
Hu, Baoli
author_sort Chen, Apeng
collection PubMed
description Understanding the glioblastoma (GBM) immune microenvironment and development of clinical treatment drugs rely on suitable preclinical GBM models. Here, we present a protocol to establish syngeneic orthotopic glioma mouse models. We also describe the steps to intracranially deliver immunotherapeutic peptides and monitor the treatment response. Finally, we show how to assess the tumor immune microenvironment with treatment outcomes. For complete details on the use and execution of this protocol, please refer to Chen et al. (2021).(1)
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spelling pubmed-100372092023-03-25 Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models Chen, Apeng Chao, Yapeng Zou, Han McCarl, Lauren H. Kohanbash, Gary Hu, Baoli STAR Protoc Protocol Understanding the glioblastoma (GBM) immune microenvironment and development of clinical treatment drugs rely on suitable preclinical GBM models. Here, we present a protocol to establish syngeneic orthotopic glioma mouse models. We also describe the steps to intracranially deliver immunotherapeutic peptides and monitor the treatment response. Finally, we show how to assess the tumor immune microenvironment with treatment outcomes. For complete details on the use and execution of this protocol, please refer to Chen et al. (2021).(1) Elsevier 2023-01-19 /pmc/articles/PMC10037209/ /pubmed/36861832 http://dx.doi.org/10.1016/j.xpro.2023.102049 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Apeng
Chao, Yapeng
Zou, Han
McCarl, Lauren H.
Kohanbash, Gary
Hu, Baoli
Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models
title Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models
title_full Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models
title_fullStr Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models
title_full_unstemmed Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models
title_short Protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models
title_sort protocol to assess the antitumor efficacy of an immunotherapeutic peptide in syngeneic orthotopic glioma mouse models
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037209/
https://www.ncbi.nlm.nih.gov/pubmed/36861832
http://dx.doi.org/10.1016/j.xpro.2023.102049
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