Protocol for in vivo CRISPR screening using selective CRISPR antigen removal lentiviral vectors

Recognition of Cas9 and other proteins encoded in delivery vectors has limited CRISPR technology in vivo. Here, we present a protocol for genome engineering using selective CRISPR antigen removal (SCAR) lentiviral vectors in Renca mouse model. This protocol describes how to conduct an in vivo geneti...

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Detalles Bibliográficos
Autores principales: Lane-Reticker, Sarah Kate, Kessler, Emily A., Muscato, Audrey J., Kim, Sarah Y., Doench, John G., Yates, Kathleen B., Manguso, Robert T., Dubrot, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037210/
https://www.ncbi.nlm.nih.gov/pubmed/36861834
http://dx.doi.org/10.1016/j.xpro.2023.102082
Descripción
Sumario:Recognition of Cas9 and other proteins encoded in delivery vectors has limited CRISPR technology in vivo. Here, we present a protocol for genome engineering using selective CRISPR antigen removal (SCAR) lentiviral vectors in Renca mouse model. This protocol describes how to conduct an in vivo genetic screen with a sgRNA library and SCAR vectors that can be applied to different cell lines and contexts. For complete details on the use and execution of this protocol, please refer to Dubrot et al. (2021).(1)

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