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Endocytosis-Like Vesicle Fission Mediated by a Membrane-Expanding Molecular Machine Enables Virus Encapsulation for In Vivo Delivery
[Image: see text] Biological membranes are functionalized by membrane-associated protein machinery. Membrane-associated transport processes, such as endocytosis, represent a fundamental and universal function mediated by membrane-deforming protein machines, by which small biomolecules and even micro...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037323/ https://www.ncbi.nlm.nih.gov/pubmed/36853954 http://dx.doi.org/10.1021/jacs.2c12348 |
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author | Uchida, Noriyuki Ryu, Yunosuke Takagi, Yuichiro Yoshizawa, Ken Suzuki, Kotono Anraku, Yasutaka Ajioka, Itsuki Shimokawa, Naofumi Takagi, Masahiro Hoshino, Norihisa Akutagawa, Tomoyuki Matsubara, Teruhiko Sato, Toshinori Higuchi, Yuji Ito, Hiroaki Morita, Masamune Muraoka, Takahiro |
author_facet | Uchida, Noriyuki Ryu, Yunosuke Takagi, Yuichiro Yoshizawa, Ken Suzuki, Kotono Anraku, Yasutaka Ajioka, Itsuki Shimokawa, Naofumi Takagi, Masahiro Hoshino, Norihisa Akutagawa, Tomoyuki Matsubara, Teruhiko Sato, Toshinori Higuchi, Yuji Ito, Hiroaki Morita, Masamune Muraoka, Takahiro |
author_sort | Uchida, Noriyuki |
collection | PubMed |
description | [Image: see text] Biological membranes are functionalized by membrane-associated protein machinery. Membrane-associated transport processes, such as endocytosis, represent a fundamental and universal function mediated by membrane-deforming protein machines, by which small biomolecules and even micrometer-size substances can be transported via encapsulation into membrane vesicles. Although synthetic molecules that induce dynamic membrane deformation have been reported, a molecular approach enabling membrane transport in which membrane deformation is coupled with substance binding and transport remains critically lacking. Here, we developed an amphiphilic molecular machine containing a photoresponsive diazocine core (AzoMEx) that localizes in a phospholipid membrane. Upon photoirradiation, AzoMEx expands the liposomal membrane to bias vesicles toward outside-in fission in the membrane deformation process. Cargo components, including micrometer-size M13 bacteriophages that interact with AzoMEx, are efficiently incorporated into the vesicles through the outside-in fission. Encapsulated M13 bacteriophages are transiently protected from the external environment and therefore retain biological activity during distribution throughout the body via the blood following administration. This research developed a molecular approach using synthetic molecular machinery for membrane functionalization to transport micrometer-size substances and objects via vesicle encapsulation. The molecular design demonstrated in this study to expand the membrane for deformation and binding to a cargo component can lead to the development of drug delivery materials and chemical tools for controlling cellular activities. |
format | Online Article Text |
id | pubmed-10037323 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-100373232023-03-25 Endocytosis-Like Vesicle Fission Mediated by a Membrane-Expanding Molecular Machine Enables Virus Encapsulation for In Vivo Delivery Uchida, Noriyuki Ryu, Yunosuke Takagi, Yuichiro Yoshizawa, Ken Suzuki, Kotono Anraku, Yasutaka Ajioka, Itsuki Shimokawa, Naofumi Takagi, Masahiro Hoshino, Norihisa Akutagawa, Tomoyuki Matsubara, Teruhiko Sato, Toshinori Higuchi, Yuji Ito, Hiroaki Morita, Masamune Muraoka, Takahiro J Am Chem Soc [Image: see text] Biological membranes are functionalized by membrane-associated protein machinery. Membrane-associated transport processes, such as endocytosis, represent a fundamental and universal function mediated by membrane-deforming protein machines, by which small biomolecules and even micrometer-size substances can be transported via encapsulation into membrane vesicles. Although synthetic molecules that induce dynamic membrane deformation have been reported, a molecular approach enabling membrane transport in which membrane deformation is coupled with substance binding and transport remains critically lacking. Here, we developed an amphiphilic molecular machine containing a photoresponsive diazocine core (AzoMEx) that localizes in a phospholipid membrane. Upon photoirradiation, AzoMEx expands the liposomal membrane to bias vesicles toward outside-in fission in the membrane deformation process. Cargo components, including micrometer-size M13 bacteriophages that interact with AzoMEx, are efficiently incorporated into the vesicles through the outside-in fission. Encapsulated M13 bacteriophages are transiently protected from the external environment and therefore retain biological activity during distribution throughout the body via the blood following administration. This research developed a molecular approach using synthetic molecular machinery for membrane functionalization to transport micrometer-size substances and objects via vesicle encapsulation. The molecular design demonstrated in this study to expand the membrane for deformation and binding to a cargo component can lead to the development of drug delivery materials and chemical tools for controlling cellular activities. American Chemical Society 2023-02-28 /pmc/articles/PMC10037323/ /pubmed/36853954 http://dx.doi.org/10.1021/jacs.2c12348 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Uchida, Noriyuki Ryu, Yunosuke Takagi, Yuichiro Yoshizawa, Ken Suzuki, Kotono Anraku, Yasutaka Ajioka, Itsuki Shimokawa, Naofumi Takagi, Masahiro Hoshino, Norihisa Akutagawa, Tomoyuki Matsubara, Teruhiko Sato, Toshinori Higuchi, Yuji Ito, Hiroaki Morita, Masamune Muraoka, Takahiro Endocytosis-Like Vesicle Fission Mediated by a Membrane-Expanding Molecular Machine Enables Virus Encapsulation for In Vivo Delivery |
title | Endocytosis-Like Vesicle
Fission Mediated by a Membrane-Expanding
Molecular Machine Enables Virus Encapsulation for In Vivo Delivery |
title_full | Endocytosis-Like Vesicle
Fission Mediated by a Membrane-Expanding
Molecular Machine Enables Virus Encapsulation for In Vivo Delivery |
title_fullStr | Endocytosis-Like Vesicle
Fission Mediated by a Membrane-Expanding
Molecular Machine Enables Virus Encapsulation for In Vivo Delivery |
title_full_unstemmed | Endocytosis-Like Vesicle
Fission Mediated by a Membrane-Expanding
Molecular Machine Enables Virus Encapsulation for In Vivo Delivery |
title_short | Endocytosis-Like Vesicle
Fission Mediated by a Membrane-Expanding
Molecular Machine Enables Virus Encapsulation for In Vivo Delivery |
title_sort | endocytosis-like vesicle
fission mediated by a membrane-expanding
molecular machine enables virus encapsulation for in vivo delivery |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037323/ https://www.ncbi.nlm.nih.gov/pubmed/36853954 http://dx.doi.org/10.1021/jacs.2c12348 |
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