Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids

We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased whe...

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Autores principales: Asad, Nadeem, Smith, Emily, Shakya, Sudeep, Stegman, Sutton, Timmons, Lisa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037626/
https://www.ncbi.nlm.nih.gov/pubmed/36961045
http://dx.doi.org/10.3390/mps6020025
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author Asad, Nadeem
Smith, Emily
Shakya, Sudeep
Stegman, Sutton
Timmons, Lisa
author_facet Asad, Nadeem
Smith, Emily
Shakya, Sudeep
Stegman, Sutton
Timmons, Lisa
author_sort Asad, Nadeem
collection PubMed
description We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness.
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spelling pubmed-100376262023-03-25 Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids Asad, Nadeem Smith, Emily Shakya, Sudeep Stegman, Sutton Timmons, Lisa Methods Protoc Technical Note We describe a workflow for efficient, environmentally attentive, and sustainable practices related to routine agarose gel electrophoresis. The methods reduce plastic waste and improve efficiency, especially for the exhaustive screening of difficult-to-obtain plasmids. Sustainability is increased when agarose is used ten times over by virtue of a thorough recycling regimen. The workflow optimizes workspaces and standardizes lab practices for handling potentially hazardous waste, minimizing environmental harm. Safety, efficiency, and sustainability improve laboratory productivity, help minimize environmental contamination, and increase cost-effectiveness. MDPI 2023-03-06 /pmc/articles/PMC10037626/ /pubmed/36961045 http://dx.doi.org/10.3390/mps6020025 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Asad, Nadeem
Smith, Emily
Shakya, Sudeep
Stegman, Sutton
Timmons, Lisa
Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids
title Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids
title_full Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids
title_fullStr Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids
title_full_unstemmed Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids
title_short Sustainable Methodologies for Efficient Gel Electrophoresis and Streamlined Screening of Difficult Plasmids
title_sort sustainable methodologies for efficient gel electrophoresis and streamlined screening of difficult plasmids
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037626/
https://www.ncbi.nlm.nih.gov/pubmed/36961045
http://dx.doi.org/10.3390/mps6020025
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