High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in

BACKGROUND: Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition. Spike-in of PhiX library ensures base diver...

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Detalles Bibliográficos
Autores principales: Naik, Tejali, Sharda, Mohak, C P, Lakshminarayanan, Virbhadra, Kumar, Pandit, Awadhesh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10037784/
https://www.ncbi.nlm.nih.gov/pubmed/36959538
http://dx.doi.org/10.1186/s12864-023-09233-4

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