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Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice

In this work, cellulose nanofibers and graphene oxide are used to fabricate a simple and reliable electrochemical biosensor, based on acetylcholinesterase (AChE) for the detection of highly dangerous organophosphates (OPs), utilizing chlorpyrifos as a representative sample. AChE is an enzyme that is...

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Autores principales: Thet Tun, Wonn Shweyi, Saenchoopa, Apichart, Daduang, Sakda, Daduang, Jureerat, Kulchat, Sirinan, Patramanon, Rina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038066/
https://www.ncbi.nlm.nih.gov/pubmed/36968027
http://dx.doi.org/10.1039/d3ra00512g
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author Thet Tun, Wonn Shweyi
Saenchoopa, Apichart
Daduang, Sakda
Daduang, Jureerat
Kulchat, Sirinan
Patramanon, Rina
author_facet Thet Tun, Wonn Shweyi
Saenchoopa, Apichart
Daduang, Sakda
Daduang, Jureerat
Kulchat, Sirinan
Patramanon, Rina
author_sort Thet Tun, Wonn Shweyi
collection PubMed
description In this work, cellulose nanofibers and graphene oxide are used to fabricate a simple and reliable electrochemical biosensor, based on acetylcholinesterase (AChE) for the detection of highly dangerous organophosphates (OPs), utilizing chlorpyrifos as a representative sample. AChE is an enzyme that is essential for neurotransmission and catalyzes the conversion of acetylcholine (ATCh) into thiocholine and acetic acid. The pesticide used in this work, chlorpyrifos, inhibits the catalytic activity of AChE on ATCh, and this inhibition can be measured using square wave voltammetry (SWV). Utilizing a process of surface modification, layers of cellulose nanofibers, graphene oxide, a chitosan-graphene oxide composite, and acetylcholinesterase (AChE/CS-GO/GO/CNFs) were immobilized on a screen-printed carbon electrode (SPCE). The modified SPCE working electrode was characterized using scanning electron microscopy and graphene oxide trapped in the cellulose nanofibers was found to increase the sensitivity of the biosensor. The modified biosensor demonstrated good performance for detection of chlorpyrifos over a linear range of 25–1000 nM under optimum conditions, and the limits of detection and quantification were 2.2 nM and 73 nM, respectively. Our sophisticated technique might offer a more precise, straightforward, quick, and environmentally friendly way to assess chlorpyrifos contamination in water and juice samples.
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spelling pubmed-100380662023-03-25 Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice Thet Tun, Wonn Shweyi Saenchoopa, Apichart Daduang, Sakda Daduang, Jureerat Kulchat, Sirinan Patramanon, Rina RSC Adv Chemistry In this work, cellulose nanofibers and graphene oxide are used to fabricate a simple and reliable electrochemical biosensor, based on acetylcholinesterase (AChE) for the detection of highly dangerous organophosphates (OPs), utilizing chlorpyrifos as a representative sample. AChE is an enzyme that is essential for neurotransmission and catalyzes the conversion of acetylcholine (ATCh) into thiocholine and acetic acid. The pesticide used in this work, chlorpyrifos, inhibits the catalytic activity of AChE on ATCh, and this inhibition can be measured using square wave voltammetry (SWV). Utilizing a process of surface modification, layers of cellulose nanofibers, graphene oxide, a chitosan-graphene oxide composite, and acetylcholinesterase (AChE/CS-GO/GO/CNFs) were immobilized on a screen-printed carbon electrode (SPCE). The modified SPCE working electrode was characterized using scanning electron microscopy and graphene oxide trapped in the cellulose nanofibers was found to increase the sensitivity of the biosensor. The modified biosensor demonstrated good performance for detection of chlorpyrifos over a linear range of 25–1000 nM under optimum conditions, and the limits of detection and quantification were 2.2 nM and 73 nM, respectively. Our sophisticated technique might offer a more precise, straightforward, quick, and environmentally friendly way to assess chlorpyrifos contamination in water and juice samples. The Royal Society of Chemistry 2023-03-24 /pmc/articles/PMC10038066/ /pubmed/36968027 http://dx.doi.org/10.1039/d3ra00512g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Thet Tun, Wonn Shweyi
Saenchoopa, Apichart
Daduang, Sakda
Daduang, Jureerat
Kulchat, Sirinan
Patramanon, Rina
Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice
title Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice
title_full Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice
title_fullStr Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice
title_full_unstemmed Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice
title_short Electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice
title_sort electrochemical biosensor based on cellulose nanofibers/graphene oxide and acetylcholinesterase for the detection of chlorpyrifos pesticide in water and fruit juice
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038066/
https://www.ncbi.nlm.nih.gov/pubmed/36968027
http://dx.doi.org/10.1039/d3ra00512g
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