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Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli
Zinc is an essential metal for cells, but excess amounts are toxic. Other than by regulating the intracellular zinc concentration by zinc uptake or efflux, the mechanisms underlying bacterial resistance to excess zinc are unknown. In the present study, we searched for zinc-resistant mutant strains f...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038286/ https://www.ncbi.nlm.nih.gov/pubmed/36961858 http://dx.doi.org/10.1371/journal.pone.0277162 |
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author | Shirakawa, Riko Ishikawa, Kazuya Furuta, Kazuyuki Kaito, Chikara |
author_facet | Shirakawa, Riko Ishikawa, Kazuya Furuta, Kazuyuki Kaito, Chikara |
author_sort | Shirakawa, Riko |
collection | PubMed |
description | Zinc is an essential metal for cells, but excess amounts are toxic. Other than by regulating the intracellular zinc concentration by zinc uptake or efflux, the mechanisms underlying bacterial resistance to excess zinc are unknown. In the present study, we searched for zinc-resistant mutant strains from the Keio collection, a gene knockout library of Escherichia coli, a model gram-negative bacteria. We found that knockout mutant of RpmJ (L36), a 50S ribosomal protein, exhibited zinc resistance. The rpmJ mutant was sensitive to protein synthesis inhibitors and had altered translation fidelity, indicating ribosomal dysfunction. In the rpmJ mutant, the intracellular zinc concentration was decreased under excess zinc conditions. Knockout of ZntA, a zinc efflux pump, abolished the zinc-resistant phenotype of the rpmJ mutant. RNA sequence analysis revealed that the rpmJ mutant exhibited altered gene expression of diverse functional categories, including translation, energy metabolism, and stress response. These findings suggest that knocking out RpmJ alters gene expression patterns and causes zinc resistance by lowering the intracellular zinc concentration. Knockouts of other ribosomal proteins, including RplA, RpmE, RpmI, and RpsT, also led to a zinc-resistant phenotype, suggesting that deletion of ribosomal proteins is closely related to zinc resistance. |
format | Online Article Text |
id | pubmed-10038286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-100382862023-03-25 Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli Shirakawa, Riko Ishikawa, Kazuya Furuta, Kazuyuki Kaito, Chikara PLoS One Research Article Zinc is an essential metal for cells, but excess amounts are toxic. Other than by regulating the intracellular zinc concentration by zinc uptake or efflux, the mechanisms underlying bacterial resistance to excess zinc are unknown. In the present study, we searched for zinc-resistant mutant strains from the Keio collection, a gene knockout library of Escherichia coli, a model gram-negative bacteria. We found that knockout mutant of RpmJ (L36), a 50S ribosomal protein, exhibited zinc resistance. The rpmJ mutant was sensitive to protein synthesis inhibitors and had altered translation fidelity, indicating ribosomal dysfunction. In the rpmJ mutant, the intracellular zinc concentration was decreased under excess zinc conditions. Knockout of ZntA, a zinc efflux pump, abolished the zinc-resistant phenotype of the rpmJ mutant. RNA sequence analysis revealed that the rpmJ mutant exhibited altered gene expression of diverse functional categories, including translation, energy metabolism, and stress response. These findings suggest that knocking out RpmJ alters gene expression patterns and causes zinc resistance by lowering the intracellular zinc concentration. Knockouts of other ribosomal proteins, including RplA, RpmE, RpmI, and RpsT, also led to a zinc-resistant phenotype, suggesting that deletion of ribosomal proteins is closely related to zinc resistance. Public Library of Science 2023-03-24 /pmc/articles/PMC10038286/ /pubmed/36961858 http://dx.doi.org/10.1371/journal.pone.0277162 Text en © 2023 Shirakawa et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Shirakawa, Riko Ishikawa, Kazuya Furuta, Kazuyuki Kaito, Chikara Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli |
title | Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli |
title_full | Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli |
title_fullStr | Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli |
title_full_unstemmed | Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli |
title_short | Knockout of ribosomal protein RpmJ leads to zinc resistance in Escherichia coli |
title_sort | knockout of ribosomal protein rpmj leads to zinc resistance in escherichia coli |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10038286/ https://www.ncbi.nlm.nih.gov/pubmed/36961858 http://dx.doi.org/10.1371/journal.pone.0277162 |
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